A high-throughput micro-droplet immobilization method and its special microfluidic chip

A microfluidic chip and immobilization method technology, which is applied in the direction of chemical instruments and methods, laboratory containers, instruments, etc., can solve the problems of complex chip design requirements, harsh chip requirements, and easy residual liquid droplets, etc., to achieve easy and flexible Combination and scale integration, simple manufacturing, fixed effect stable effect

Inactive Publication Date: 2011-11-30
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, dielectric force immobilization is easy to cause damage to the organisms in the droplet; immobilization using hydrophilic and hydrophobic properties is more demanding on chip manufacturing, and it is easy to remain and cause contamination between droplets; When fixed, the chip design requirements are complex, and the fixed system is unstable and easily disturbed
All three have certain limitations

Method used

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  • A high-throughput micro-droplet immobilization method and its special microfluidic chip
  • A high-throughput micro-droplet immobilization method and its special microfluidic chip
  • A high-throughput micro-droplet immobilization method and its special microfluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A high-throughput microdroplet immobilization method on a microfluidic chip based on the principles of buoyancy and surface tension and a dedicated chip. The specific process of the method is:

[0030] The continuous phase and dispersed phase inlets on the chip are respectively connected to two syringe pumps through Teflon tubes. Driven by the syringe pump, the continuous phase and the dispersed phase are injected into the chip respectively, and then the T-shaped droplets are formed in the generation area. Continuous monodisperse droplets;

[0031] The continuously generated droplets continue to flow under the drive of the syringe pump and enter the droplet catcher array. When the droplets flow through the lower channel of the catcher, they enter the cylindrical catcher upwards due to the combined action of buoyancy and surface tension. It flows through the lower channel and is sequentially captured in the droplet catcher of the cylinder;

[0032] After the droplets w...

Embodiment 2

[0037] The high-throughput micro-droplet immobilization method on a microfluidic chip based on the principles of buoyancy and surface tension uses a dedicated microfluidic chip. The lower layer is the T-shaped droplet generation area and the droplet evacuation channel.

[0038] In the specific operation, we connect the continuous phase and dispersed phase inlets on the chip to two syringe pumps respectively through Teflon tubes (the density of the continuous phase is greater than that of the dispersed phase), and driven by the syringe pump, the continuous phase and dispersed phase The phases are injected into the chip separately, and continuous monodisperse microdroplets are formed in the lower T-shaped microdroplet generation area; the generated microdroplets continue to flow into the droplet catcher array, and are sequentially one by one under the combined action of buoyancy and surface tension. Captured in the upper cylindrical droplet catcher.

[0039] The dedicated micro...

Embodiment 3

[0041] A high-throughput micro-droplet fixing method, based on the principles of buoyancy and surface tension, is manipulated on a microfluidic chip; the micro-droplet fixing method is specifically:

[0042] The continuous phase and dispersed phase inlets on the chip are respectively connected to two syringe pumps through Teflon tubes. Driven by the syringe pump, the continuous phase and the dispersed phase are injected into the chip respectively, and then the T-shaped droplets are formed in the generation area. Continuous monodisperse droplets (the density of the continuous phase is greater than the density of the dispersed phase);

[0043]The continuously generated microdroplets continue to flow under the drive of the syringe pump and enter the droplet catcher array. When the droplets flow through the lower channel of the catcher, they enter the cylindrical catcher upwards due to the combined action of buoyancy and surface tension; The lower channel of the droplet catcher th...

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Abstract

A high-throughput micro-droplet immobilization method, based on the principle of buoyancy and surface tension, is controlled on a microfluidic chip. The micro-droplet immobilization method specifically includes: injecting the continuous phase and the dispersed phase into the chip using a syringe pump, As a result, continuous monodisperse microdroplets are formed in the T-shaped microdroplet generation zone; the microdroplets continue to flow and enter the droplet catcher array driven by the syringe pump, and the droplets flow through the channel below the catcher due to buoyancy and The combined effect of surface tension enters the cylindrical catcher upwards; subsequent droplets flow through the lower channel of the droplet catcher that has successfully immobilized the droplet, and are sequentially captured in subsequent droplet catchers. The invention simplifies the principle and process of liquid droplet immobilization, the microfluidic chip involved is simple to manufacture, convenient to operate in experiments, stable in immobilization effect, does not cause damage to organisms in the liquid droplet, and is easy to carry out with other functional units on the chip. Flexible combination and scale integration.

Description

Technical field: [0001] The invention relates to a droplet positioning and fixing technology in a microfluidic chip, and particularly provides a high-throughput microdroplet fixing method and a dedicated microfluidic chip. Background technique: [0002] In the prior art, a microfluidic chip, also known as a lab on achip, refers to the integration of basic operating units such as sample preparation, reaction, separation, and detection involved in the fields of biology and chemistry. Or basically integrated on a chip of a few square centimeters (or even smaller), a network of microchannels is formed to run through the entire system with controllable fluid, a technology used to replace various functions of conventional biological or chemical laboratories. Its basic feature and greatest advantage is the flexible combination and scale integration of multiple unit technologies on tiny platforms. Up to now, microfluidic chip technology has been applied in different fields of life ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00B01J19/00G01N35/00
Inventor 秦建华施维维温慧陆瑶林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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