Droplet microfluidic device and methods of sensing the result of assay therein

一种微流控、液滴的技术，应用在生物化学设备和方法、微生物的测定/检验、流体控制器等方向，能够解决未公开TFT背板电路实施例等问题，达到低成本的效果

Active Publication Date: 2018-04-17
SHARP LIFE SCI EU LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The disadvantage of US7163612 is that it does not disclose any circuit embodiment of TFT backplane for realizing AM-EWOD

Method used

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  • Droplet microfluidic device and methods of sensing the result of assay therein
  • Droplet microfluidic device and methods of sensing the result of assay therein
  • Droplet microfluidic device and methods of sensing the result of assay therein

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0064] In the drawings, like reference numerals indicate like components or features.

[0065] figure 2 An exemplary assay measurement system according to a first embodiment of the present invention is shown. The measurement system includes two components, such as a reader 40 and a cartridge 44 . Cassette 44 may contain a microfluidic device (eg, AM-EWOD device 41 ) as well as (not shown) fluidic input ports and electrical connections into the device. The fluid input port may perform the function of inputting fluid into the AM-EWOD device 41 and dispensing from an input reservoir, for example by electrowetting control, to generate droplets 4 within the device. 15. As further detailed below Yes, the microfluidic device includes an electrode array configured to receive an input fluid droplet.

[0066] The assay measurement system may further include a controller configured to control an actuation voltage applied to the electrode array of the microfluidic device to perform a m...

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Abstract

A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagentdroplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.

Description

technical field [0001] The present invention relates to droplet microfluidic devices, and in particular to electrowetting on dielectric (EWOD) devices, and more particularly to active matrix electrowetting on dielectric (AM-EWOD), and to sensing in A method of determining the dynamic properties of one or more droplets on such a device as a result of chemical or biochemical testing. Background technique [0002] Electrowetting on dielectric (EWOD) is a well known technique for manipulating droplets of a fluid by applying an electric field. Active-matrix EWOD (AM-EWOD) refers to realizing EWOD in an active-matrix array incorporating transistors, for example by using thin film transistors (TFTs). Therefore, it is a candidate technology for digital microfluidics for lab-on-a-chip technology. An introduction to the rationale for this technology can be found in: "Digital microfluidics: is a true lab-on-a-chippossible?", R.B. Fair, Microfluid Nanofluid (2007) 3:245-281. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/08G01N1/00G01N33/86G01N37/00
CPCB01L3/502792B01L2200/148B01L2300/0819B01L2300/1822B01L2400/0677G01N30/6095G01N2030/8813G01N33/579C12Q1/6844G01N33/4905B01L2300/0645B01L2400/0427B01L2200/14C12Q2563/116C12Q2563/159C12Q2565/607C12Q2565/629C12Q1/6816
Inventor 本杰明·詹姆斯·哈德文艾德里安·马克·西蒙·雅各布詹森·罗德里克·赫克托迈克尔·詹姆斯·布朗洛足立昌浩艾莉森·玛丽·斯金纳马克·蔡尔兹
Owner SHARP LIFE SCI EU LTD
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