Multimode coupling in-situ microscopic spectral imaging system

Abstract

The invention discloses a multimode coupling in-situ microscopic spectral imaging system. The system is composed of an inverted microscope, an exciting light source module, a light path switching module, a spectral imaging and spectrum test module, and refers to a plurality of spectrum and imaging measurement functions in the same micron-order area of a sample, comprising measuring microcell Ramanspectrum and microcell fluorescence spectrum, fluorescence microimaging, microcell transmittance spectrum, transmittance microimaging, microcell reflection spectrum and reflection microimaging. The system has the characteristics that multimode and multidimensional in-situ detection and analysis can be performed on the same microcell of the sample with no need to moving the sample to various kindsof detection equipment; the acquired sample microcell information can be represented in situ, namely that various kinds of information such as the Raman spectrum, fluorescence spectrum and imaging, transmittance spectrum and imaging, reflection spectrum and imaging of the same microcell of the sample can be acquired in real time. Measurement errors caused by transferring of the sample are eliminated, and the reliability of the test result is greatly improved.

Description

technical field [0001] The invention belongs to the field of optical analysis and detection, and in particular relates to a multi-mode coupling in-situ microspectral imaging system. Background technique [0002] Microspectroscopy and imaging technology mainly includes analysis and testing technologies such as Raman spectroscopy, fluorescence spectroscopy and imaging, transmission spectroscopy and imaging, and reflectance spectroscopy and imaging of sample micro-regions. The Raman spectrum of a substance can reflect the molecular structure information of the substance; the fluorescence spectrum and imaging can reflect the energy level information of the substance; the transmission spectrum and imaging and reflection spectrum and imaging can intuitively and accurately reflect the color and color change of the substance. Microspectroscopy and imaging technology not only has the ability of spatial resolution, but also has the ability of spectral resolution, and can know the comp...

AI Technical Summary

Problems solved by technology

[0003] However, in practical applications, the various spectral analysis and testing technologies in microspectroscopy and imaging technology are independent of each other, and it is necessary to move samples to different instruments for analysis and testing, resulting in inevitable measurement errors

Existing instruments and equipment systems cannot meet the needs of multiple in situ microspectroscopy and imaging measurements on the same micro-region of the sample

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