Single cell microoperation apparatus for microscopic injection

Abstract

The invention discloses a unicellular micro-operation device used for microinjection. An air supply and a pressure control valve of the device and a left and a right end effectors form a sealed air course through a passage in a holder; the left and the right end effectors are respectively connected with a left and a right three dimensional micromotion stages through the holders; the left and the right three dimensional micromotion stages are symmetrically arranged on the left side and the right side of an inverted microscope worktable; the left and the right end effectors form a unicellular micro-operation flow field with controllable flow rate distribution on the inverted microscope worktable; and a computer collects signals of an image detecting and processing unit to respectively control the pressure control valve and the left and the right three dimensional micromotion stages. The micro-operation device effectively controls the operation environmental flow field of cells, and forms a stable unicellular micro-operation flow field with controllable flow rate distribution to drive the cells to move. Through the invention, the cellular angle in the three dimensional orthogonalized plane can be randomly adjusted, so that the three degrees of freedom (DOF) attitude of the cells can be accurately controlled; and the three dimensional spatial location of the cells is adjusted to realize the accurate positioning of the location and attitude of the cells.

Description

technical field [0001] The invention belongs to microsystem technology, and relates to a single-cell micromanipulation device based on the coupling effect of mechanical motion and flow field, in particular to a single-cell micromanipulation device for microinjection. Background technique [0002] In different cell engineering applications, the operation targets of microinjection on cells are different, such as female pronucleus for pronuclear injection of fertilized egg, polar body for oocyte polar body reorganization, and spindle for single sperm microinjection, etc. Precisely locate the position and posture of the manipulated cells. The existing single-cell micromanipulation methods are divided into two types: contact type and non-contact type, namely: [0003] 1. The contact type is mainly based on the mechanical operation mode of the existing manual adjustment of the microinjection system, such as the NT-88NE microinjection system of the Japanese Narishige company. The...

AI Technical Summary

Problems solved by technology

The existing contact mechanical operation method has the following disadvantages: it is difficult to adjust the position and posture of cells, it is seriously affected by the operator's subjective factors, the training period for operators is long, the cost is high, the automation is poor, and the cell posture cannot be adjusted accurately. The efficiency of microscopic experiments is low, it is easy to damage the manipulated cells, and the success of experiments is low

Therefore, the non-contact cell micromanipulation method cannot meet the application requirements of cell microinjection

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