Disclosed is a method of adjusting an image reading apparatus which reads image information of an original by moving relative to the original a carriage retaining a light source, a slit portion, a plurality of mirrors, an imaging device including an optical element with an optical surface rotationally asymmetrical with respect to the optical axis, and a reading device, the method including placing an adjustment chart allowing resolving power evaluation in the sub scanning direction on an original table glass, illuminating the adjustment chart by the light source, and adjusting, by using an image of the adjustment chart thus illuminated, the relative positional relationship in the sub scanning direction among the slit portion, the imaging device, and the reading device by a positioning adjustment device such that imaging of the light beam passing through a slit center of the slit portion is effected on the surface of the reading device. By performing information through adjustment using the adjustment chart, there are provided an adjusting method of image reading apparatus and an image reading apparatus which provide high quality image.