The invention relates to a novel measurement method and the device thereof, which combines the time-resolved self-
fluorescence lifetime
imaging technology and a
laser scanning
confocal ophthalmoscopeand realizes the early diagnosis of the ocular fundus diseases by obtaining and analyzing the self-
fluorescence intensity and the service life information of the ocular fundus. The light which is emitted by a pulse
laser is sent to the scanning head of the
laser scanning
confocal ophthalmoscope to excite the ocular fundus inner-source flgorgen, and the
fluorescence which is emitted from the ocularfundus is collected by a time-correlated single
photon counter or a high-repetition-rate synchronous scanning camera, so as to realize the service life measurement and the imaging of the self-fluorescence. As the service life of the self-fluorescence of the ocular fundus is very sensitive to the micro-environment and the
cell metabolism state of flgorgen molecule and is not affected by the difference of the intensity of the flgorgen of the ocular fundus, the measurement of the fluorescence service life provides a brand new
route for the detection of ocular fundus diseases and the obtainment of the function information of ocular fundus. The invention can become the solution and the basis of the early diagnosis of various ocular fundus diseases, which plays an important role in pathogeny research,
clinical diagnosis, staging and
typing, prognosis judgement, real-time observation and the analysis of the development of ocular fundus diseases, therefore the invention can be widely appliedin the field of
medical research and clinical
medical diagnosis.