Cell cultivation device applied to microscopic observation

A cell culture and microscopic observation technology, which is applied in tissue cell/virus culture devices, enzymology/microbiology devices, biochemical cleaning devices, etc. effect, no cell reaction method, etc., to achieve the effect of excellent conductivity, reduced interference factors, and suitable thickness

Inactive Publication Date: 2016-02-17
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the cell experiment method of electric field effect is mainly based on the inter-group comparison of the observation results after electric field exposure, but it is still difficult to confirm the cell effect caused by electric field, especially it is difficult to rule out other exogenous interference factors other than e...

Method used

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  • Cell cultivation device applied to microscopic observation
  • Cell cultivation device applied to microscopic observation
  • Cell cultivation device applied to microscopic observation

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with accompanying drawing. However, the following embodiments are only preferred embodiments of the present invention, not all of them.

[0035] Such as figure 1 , figure 2Shown, a kind of cell culture device that is used for microscopic observation of the present invention comprises incubator 4, and incubator 4 is cuboid structure, comprises the box body and cover body 41 that four sides and bottom plate 42 are formed, and the incubator 4 The box body and the cover body 41 form a sealed chamber through the sealing ring 7 . The bottom plate 42 and the cover 41 of the incubator are transparent PDMS. Both the first conductive layer 31 and the second conductive layer 32 are provided on the inner surfaces of the cover body 41 and the bottom plate 42. The first conductive layer 31 and the second conductive layer 32 are made of graphene material, and are respectively connected to the UHV DC power supply ...

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Abstract

The invention discloses a cell cultivation device applied to microscopic observation. The cell cultivation device comprises a cultivation tank, wherein a cover body is arranged at the top of the cultivation tank, the cover body and a base plate of the cultivation tank are made of transparent materials, a first conducting layer and a second conducting layer are respectively arranged on the inner side faces of the cover body and the base plate and are connected with electrodes of a power source, and a space between the first conducting layer and the second conducting layer after being powered on forms an electric field; a culture solution inlet and outlet are formed in the tank body of the cultivation tank. According to the cell cultivation device applied to the microscopic observation, the two conducting layers are arranged in parallel to generate a static electric field; an inverted microscope is utilized to in-situ observe a cell biological process exposed in a strong static field in real time, so that the interference factor is reduced largely, and objective evaluation of the biological effect of the static field on a cell level is facilitated.

Description

technical field [0001] The invention relates to the field of electric field biological effect research, in particular to a cell culture device for microscopic observation. Background technique [0002] With the construction of UHV DC transmission projects and the continuous improvement of residents' requirements for their own environment, the electromagnetic field generated by UHV DC transmission lines and its health effects have attracted public attention, and the formulation of relevant standards for UHV DC transmission technology is also increasing. urgent. Different from the time-varying electromagnetic field generated by the alternating current transmitted by the AC transmission line, the constant current transmitted by the DC transmission line produces a 0Hz time-invariant electric field (electrostatic field). [0003] At present, there have been a lot of researches at home and abroad on the time-varying electromagnetic fields generated by AC power transmission and tr...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/42C12M1/38C12M1/36C12M1/34
Inventor 翟国庆刘广祥江建华辛亮
Owner ZHEJIANG UNIV
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