Confocal laser optical tweezers Raman spectroscopy test device capable of being used in upright and inverted manners
An optical tweezers Raman and spectral testing technology, which is applied in the field of cell or organelle research, can solve the problems of high laser power, inability to make full use of the numerical aperture of the oil lens lens, and the inability of cells to be captured by optical tweezers.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0017] First, the single-mode semiconductor Raman laser outputs 785nm laser light, which is reflected by mirror A 1 and mirror B 23 and then passes through convex lens A 22, pinhole 21, convex lens B 20, band-pass color filter 19, mirror C 18 and reflector After the mirror D 3, the laser light is incident on the 785nm short-wave pass dichroic beam splitter 4 with an incident angle of 45° and is transmitted through the beam splitter, and the laser light is introduced into a reflective mirror E 5 equipped with a 45° reflection angle to reflect and enter Upright the microscope 11 until the 63 times Leica water mirror lens in the upright microscope 11, the emitted laser captures the active cells in the cell culture dish A 12 under the water mirror, and the laser power is about 10mw at this time. Use the built-in CCD in the microscope to observe whether the cells are stably trapped in the potential well formed by the laser focus. When the cell is trapped in the optical tweezers wel...
Embodiment 2
[0020] Turn the upper reflector E5 in the upright microscope 11 to move out of the optical path, so that the laser light is directly emitted to the reflector F6, and then reflected by the reflector G10 and the reflector H9, the laser light is introduced into the inverted microscope 8. The 100x oil immersion lens with a high numerical aperture configured in the microscope 8 can more easily realize the optical tweezers capture and Raman spectrum testing of cells.
[0021] The above-mentioned laser is a 785nm single-mode semiconductor Raman laser (manufacturer: Shanghai Xilong Optoelectronics Technology Co., Ltd., model: SL-785-XXX), and the maximum power of the emitted laser is 100mW; the upright microscope is Leica brand; Kaiser spectrometer (model: HoloSpec-f_2.2-NIR); mirror model: PF10-03-P01-10 spatial filter (KT310), convex lens (model: LA1608-B-ML), bandpass filter (model: FL780-10) , dichroic beam splitter (LPD01-785RU-25), notch filter (NF01-785U-25), purchased from Tho...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com