Optical super-resolution imaging system based on inverted microscope and micro-sphere lens and dynamic imaging method using same

Abstract

The invention discloses an optical super-resolution imaging system based on an inverted microscope and a micro-sphere lens which can realize optical super-resolution imaging under an inverted microscope. The optical super-resolution imaging system includes an optical mirror, a micro-sphere lens, an atomic force microscope scanning probe, a sample stage, an optical inverted microscope, and a displacement stage. The atomic force microscope scanning probe is pasted through a substrate thereof onto the optical mirror. The micro-sphere lens is fixed to a cantilever beam and a tip of the atomic force microscope scanning probe, and is positioned below the optical mirror. The optical mirror is fixed to the displacement stage above the optical mirror. The sample stage is positioned below the micro-sphere lens. The optical inverted microscope is positioned below the sample stage, and the objective lens of the optical inverted microscope is positioned right below the micro-sphere lens. The invention further provides a dynamic imaging method using the system. The system of the invention is especially suitable for observing the internal structure of living cells and other occasions.

Description

technical field [0001] The invention relates to an optical super-resolution imaging system and method based on an inverted microscope and a microsphere lens. Specifically, the optical super-resolution microscopic observation and imaging under an inverted microscope is realized by using the control mechanism of a displacement stage, and macro, micro, and nano imaging can be realized. Seamless observation, and nanomanipulation with real-time visual feedback. It is mainly used in fields that require nanoscale super-resolution real-time dynamic observation and operation, including materials, life sciences and other fields. Background technique [0002] At the end of the 19th century, the German scientist Ernst Abbe defined the resolution of the optical microscope, which was considered to be half the wavelength of light, that is, about 0.2 microns, which is the famous optical diffraction limit. Thus, for most of the 20th century, scientists believed that optical microscopy would...

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Problems solved by technology

Under an upright microscope, these transformed propagating waves are captured and imaged by the objective lens; while under an inverted microscope, these transformed propagating waves are far away from the objective lens of the microscope, so they cannot be collected and imaged by the objective lens

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