Osteoblast electrical stimulation system based on microfluidic technology and operation method thereof

A microfluidic technology and osteoblast technology, applied in the field of cell microenvironment reconstruction in vitro, can solve the problems of inability to analyze a single osteoblast, and cannot accurately evaluate the effect of a single osteoblast, so as to eliminate adverse effects and reduce fluid loss. Surface fluctuations, cost reduction effect

Active Publication Date: 2014-05-14
INST OF ELECTRONICS CHINESE ACAD OF SCI
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Problems solved by technology

[0008] Although many scholars have used microfluidic technology to stimulate osteoblasts and observe cell responses, none of these experiments and metho

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  • Osteoblast electrical stimulation system based on microfluidic technology and operation method thereof
  • Osteoblast electrical stimulation system based on microfluidic technology and operation method thereof
  • Osteoblast electrical stimulation system based on microfluidic technology and operation method thereof

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preparation example Construction

[0045] In another exemplary embodiment of the present invention, a method for preparing a microfluidic chip based on microfluidic technology is also provided. figure 2 for figure 1 Flowchart of the preparation method of the microfluidic chip in the osteoblast electrical stimulation system shown. Please refer to figure 2 , the preparation method comprises:

[0046] Step A, making the master mold of the microfluidic chip;

[0047] The step A of making the master model of the microfluidic chip can further include:

[0048] In sub-step A1, the slides were ultrasonically cleaned in acetone (40W, 3 minutes), ultrasonically cleaned in ethanol (40W, 3 minutes) and deionized water (40W, 3 minutes), and then placed on the Dry on a hot plate (150°C, 30 minutes), as Figure 3A As shown in neutron diagram A-1;

[0049] Sub-step A2, evenly spin-coat a layer of SU-85 photoresist on the surface of the glass slide (1500RPM, 35 seconds), then perform prebaking (65°C, 2 minutes; 95°C, 5 ...

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Abstract

The invention provides an operation method of an osteoblast electrical stimulation system based on a microfluidic technology. The osteoblast electrical stimulation system comprises a microfluidic chip, an inverted microscope, two elastic sealing plugs and an electrical stimulation signal source, wherein the microfluidic chip is prepared from a transparent material and is provided with a micro-channel which is used for bearing a cell culture medium and providing space environments for the culture of osteoblast, and an inlet and an outlet are respectively formed in the two ends of the micro-channel; a camera lens of the inverted microscope is aligned at the middle part of the micro-channel from the bottom of the microfluidic chip; the two elastic sealing plugs are used for respectively plugging the inlet and the outlet of the micro-channel; the electrical stimulation signal source is used for providing electrical stimulation signals, and the two electrodes of the electrical stimulation signal source respectively penetrate through the two elastic sealing plugs and then extend below the liquid level of the cell culture medium in the micro-channel. The osteoblast electrical stimulation system provided by the invention can be used for realizing the analysis of single osteoblast and assessing the influencing effect of different potential differences on the single osteoblast.

Description

technical field [0001] The invention relates to the technical field of cell microenvironment reconstruction in vitro, in particular to an osteoblast electrical stimulation system based on microfluidic technology and an operating method thereof. Background technique [0002] Bone nonunion or delayed union is a serious complication after fracture, and bone formation disorder is one of the main reasons. Bone formation is a complex phenomenon, which refers to the recruitment and replication of mesenchymal osteoblast progenitor cells, further differentiation into pre-osteoblasts and mature osteoblasts, and finally leads to the accumulation and mineralization of extracellular matrix. Osteoblasts play a key role in this process, so it is very important to study how to promote the proliferation of osteoblasts. [0003] In recent years, many scholars have confirmed that a variety of stimulation methods can promote the proliferation of osteoblasts, such as electricity, microwave vibr...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12N13/00
CPCC12M23/16C12M25/08C12M35/02C12N5/0654C12N2529/00
Inventor 陈健祁宝昌卫超赵阳卫元晨陈德勇王军波
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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