Tetrahydroxanthone dimer compound as well as preparation method and application thereof

A xanthone dimer and compound technology, which is applied in the field of tetrahydroxanthone dimer compounds, can solve problems such as no anti-Helicobacter pylori activity, and is convenient for pharmacological and clinical research, extraction and separation Simple method, good effect

Active Publication Date: 2022-04-08
ZHEJIANG UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention obtained a new bacterial strain-the basket-shaped fungus WHUF0362 of marine origin. The tetrahydroxanthone dimer compound obtained through fermentation and cultivation has not been found at home and abroad so far related patents or anti-helicobacter pylori activity Literature report

Method used

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  • Tetrahydroxanthone dimer compound as well as preparation method and application thereof
  • Tetrahydroxanthone dimer compound as well as preparation method and application thereof
  • Tetrahydroxanthone dimer compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Screening and identification of bacterial strain WHUF0362

[0031] 1. Strain screening

[0032] (1) The mangrove candela rhizosphere soil samples collected from Yalong Bay, Sanya City, Hainan, China in December 2018 were diluted step by step with sterile water to 10-1 , 10 -2 , 10 -3 For different gradients, each dilution is vortexed for 3-5 minutes before the next dilution; the samples of different dilution gradients are evenly coated on the MEA medium with a coating rod, and placed in a 30°C constant temperature incubator for upside-down culture . Three parallel experiments were set up for each sample.

[0033] (2) In the following 1 to 7 days, observe the culture of step (1) and pick different types of single colonies and repeat step (1) to streak and separate until it is determined to be a pure single colony.

[0034] (3) Inoculate the different single colonies of step (2) on PDA solid medium respectively, and after culturing at 28°C for 24 hours, cut...

Embodiment 2

[0050] Embodiment 2, the preparation of formula (I) compound

[0051] 1. Fermentation culture

[0052] Pick the basket-shaped fungus WHUF0362 strain stored in the cryopreservation tube, inoculate it into a plate medium, and cultivate it in an incubator at 28°C for 3 days until the colony grows to maturity. Inoculate the ISP with spores 4 In the liquid medium, shake culture at 28° C. and 200 rpm for 4 days, and serve as seed solution. The seed solution was respectively inserted into sterilized 1 L Erlenmeyer flasks containing 200 g of rice culture medium with an inoculation amount of 5% volume concentration, a total of 60 flasks. The fermented product was obtained by statically culturing at room temperature for 15 days.

[0053] The final concentration of the plate medium is composed of: potato 200g / L, glucose 20g / L, agar 18g / L, solvent is water, and natural pH.

[0054] ISP 4 The final concentration of the liquid medium consists of: soluble starch 15g / L, glucose 5g / L, pep...

Embodiment 3

[0074] Embodiment 3: Formula (I) compound antibacterial activity in vitro

[0075] (1) Preparation of the test bacteria solution: select the monoclonal colony of Helicobacter pylori 26695 (H. pylori26695) grown on the solid medium, inoculate it on the Columbia plate medium, culture it at 37°C for 2 days and grow to the stationary phase, then Inoculate into fresh BHI medium containing 10% calf serum in a volume ratio of 1:100, and culture at 37°C for 1d to mid-late logarithmic (OD 600nm =0.8-1.0), the obtained concentration is 10 6 CFU / mL of bacterial liquid.

[0076] Preparation of Helicobacter pylori G27 (H.pylori G27), Helicobacter pylori 159 (H.pylori 159) and Helicobacter pylori 129 (H.pylori 129) bacteria liquid: Helicobacter pylori 26695 were replaced with Helicobacter pylori G27 (H.pylori G27, purchased from American Type Culture Collection ACTT), Helicobacter pylori 159 (H.pylori 159, purchased from American Type Culture Collection ACTT) and Helicobacter pylori 129 (...

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Abstract

The invention discloses a tetrahydro-oxanthone dimer compound as shown in a formula (I), a preparation method of the tetrahydro-oxanthone dimer compound and application of the tetrahydro-oxanthone dimer compound to preparation of antibacterial agents, talaromyces sp. WHUF0362 screened by the invention is convenient to culture and easy to survive, the compound (I) extracted by fermentation of the talaromyces sp. WHUF0362 has a certain inhibition effect on helicobacter pylori, the MIC (minimal inhibitory concentration) value reaches 0.125 mu g / mL, and the compound can be used for preparing antibacterial agents. The extraction and separation method is simple and easy, further pharmacological and clinical research is facilitated, and conditions are created for developing novel antibacterial drugs with good curative effects and small toxic and side effects.

Description

(1) Technical field [0001] The invention relates to a tetrahydroxanthone dimer compound and its preparation method and application. (2) Background technology [0002] Marine fungi are a class of microorganisms that can form spores and have eukaryotic structures that live in the ocean. Most fungi live on some kind of substrate. The distribution of fungi in the ocean mainly depends on the distribution of the host. They are distributed in intertidal high tide lines, estuaries, shallow sea beaches, deep sea sediments and many marine organisms. Marine fungal secondary metabolites are characterized by rich and diverse structures, broad spectrum of biological activity and high intensity, and are an important source of marine active natural products. Biological activities mainly include antibacterial, antitumor, antioxidative, and enzyme inhibitory activities. For example, the antibiotic cephalosporin C, which is widely used in clinic, is isolated from marine fungi. [0003] Wu e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/86A61P31/04C12P17/16C12N1/14C12R1/645
CPCY02A50/30
Inventor 吕华伟洪葵苏海波薛雅馨李行诺
Owner ZHEJIANG UNIV OF TECH
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