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Novel triterpenoid compound and preparation method thereof

A technology of triterpene compounds and compounds, applied in the field of medicine, can solve problems such as no patents or literature reports, and achieve the effects of facilitating pharmacological and clinical research, inhibiting tumor cell growth, and novel structure

Inactive Publication Date: 2011-12-07
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Novel triterpene compounds involved in the present invention and their activity have not been patented or reported in the literature so far

Method used

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  • Novel triterpenoid compound and preparation method thereof
  • Novel triterpenoid compound and preparation method thereof
  • Novel triterpenoid compound and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the preparation of formula (I) compound:

[0035] Using 10 kg of dried medicinal material from the root of Schisandra chinensis as raw material, it was heated and refluxed with 90% ethanol for 3 times, each time for 2 hours, and concentrated to obtain 1500 g of extract-like ethanol extract. The ethanol extract was suspended in water, and extracted 4 times with petroleum ether, 1000 mL each time. The extract from the petroleum ether extraction part was separated by silica gel (200-300 mesh) column chromatography, and gradient eluted with petroleum ether-ethyl acetate (the volume ratio of the eluent was 100:1-1:1), and the petroleum The eluted part with a volume ratio of ether-ethyl acetate of 100:3 was separated by silica gel (200-300 mesh) column chromatography, and petroleum ether-acetone (the volume ratio of the eluent was 100:1-1:1) Gradient elution, the eluted part with a volume ratio of petroleum ether-acetone of 100:3 was purified by Sephadex LH-20 ...

Embodiment 2

[0036] Example 2: In vitro growth inhibition test of the compound of formula (I) on human acute promyelocytic leukemia cell line HL-60:

[0037] HL-60 cells were cultured in 10% heat-inactivated fetal bovine serum, 100 IU / mL penicillin, 100 m g / mL streptomycin and 1 mmol / L L -Glutamine in RPMI1640 medium, 37 °C, 5% CO 2 Incubate in a saturated humidity incubator. Weigh trypan blue, add a small amount of distilled water to grind, add double distilled water to dilute to 4% storage concentration, filter with filter paper, and store at 4 °C. When used, this stock solution was diluted to a working concentration of 0.4% with PBS. Take the above cells (1'10 5 / mL) were inoculated in a 12-well plate, 2 mL per well. Add different concentrations of drugs to prepare a single cell suspension after incubation, take 50 mu L cell suspension added 50 mu 0.4% trypan blue solution in L, mix well, and observe under the microscope within 3 minutes, the dead cells are stained blue, while ...

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Abstract

The invention relates to a novel triterpenoid compound extracted and separated from Kadsura coccinea roots and application thereof in preparation of human acute promyelocytic leukemia cell line HL-60 inhibitors. The compound has a chemical structure formula shown in a formula I. The experimental study shows that the compound has growth inhibition action on human acute promyelocytic leukemia cell line HL-60, and the extraction and separation method of the compound is simple, thereby facilitating the further pharmacological and clinical research of the compound and the development of the application of the compound in preparation of antitumor drugs. The formula I is shown in the specification.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a new triterpene compound and a preparation method thereof, in particular to a new triterpene compound extracted and separated from the root of the medicinal plant Schisandra schisandra and its use in the preparation of human acute promyelocytic leukemia cells The use of strain HL-60 inhibitors. Background technique [0002] Tumor is one of the most serious diseases that seriously threaten human life. For years, scientists have been working to unravel the mysteries of how tumors occur. Therefore, studying tumors and overcoming cancer has become an important research topic in the medical field. The diversity of active substance structures in plants and the wide range of activities make screening anti-tumor active substances from plants a research hotspot. Scholars at home and abroad have found many anti-tumor substances by screening tens of thousands of herbal medicines. Studies ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C57/26C07C51/47A61K31/19A61P35/02
Inventor 华会明李占林王楠裴月湖白皎景永奎
Owner SHENYANG PHARMA UNIVERSITY
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