Juglans mandshurica bark extract and application thereof in preparing anticancer drugs
A technology of walnut catalpa and its extract is applied to the bark extract of walnut catalpa and its application field in the preparation of anticancer drugs, which can solve the problem of complex composition of walnut catalpa bark, lack of systematic and in-depth exploration, and activity detection model only Limited to problems such as animals and tissues, the extraction and separation method is simple and easy to implement, enhances social productivity, and promotes the health of the social population.
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Embodiment 1
[0029] Take 500g of walnut catalpa bark (dry product), put it in a round bottom flask after crushing, add 4000ml of 70% ethanol, put it in an electric heating mantle and add a condenser tube to form a reflux system, repeat the reflux extraction for 3 times, the first 1000ml reflux for 1h, and then 3000ml was refluxed twice for 2.5h, and a total of 4000ml of ethanol solution with a mass fraction of 70% was used for reflux extraction for 3.5h. Use a rotary evaporator to recover ethanol under reduced pressure and concentrate the extract to a thick paste, add 5 times the amount of water to dissolve and disperse. After dissolving in water, use petroleum ether, ethyl acetate, and n-butanol for fractional extraction, and extract each phase 10 times, 300ml each time, for a total of 3000ml extract. The extract was distilled to dryness under reduced pressure to obtain petroleum ether components, ethyl acetate components, n-butanol components and water phase components respectively. Take ...
Embodiment 2
[0031] Take 500g of dried walnut catalpa bark, put it in a round-bottomed flask and add 5000ml of 70% ethanol to fully soak it, put it in an electric heating mantle and add a condenser tube to form a reflux system, and repeat the reflux extraction for 3 times, about 1700ml each time. About 1.5h, a total of reflux extraction 4.5h. Use a rotary evaporator to recover ethanol under reduced pressure and concentrate the extract to a thick paste, add 5 times the amount of water to dissolve and disperse. The solution was sequentially extracted with petroleum ether, ethyl acetate and n-butanol, 10 times each, and the extract was distilled to dryness under reduced pressure. The petroleum ether component, the ethyl acetate component, the n-butanol component and the water phase were respectively obtained. The anti-tumor cell activity test of above-mentioned four extraction phases, the results are shown in Table 1, the results show that the petroleum ether component, ethyl acetate compone...
Embodiment 3
[0036] Grind the bark of Catalpa walnut, take 500g of bark powder and extract 3 times with 70% ethanol under reflux, reflux with 4000ml for 3 hours for the first time, and reflux with 3000ml for 2.5h twice, use 7000ml of ethanol solution with a mass fraction of 70% for 5.5h in total . The extracts were combined, concentrated under reduced pressure, and the extract was collected. Dissolve with 5 times water, filter with suction, and save the filter residue. The filtrate was extracted with diethyl ether, ethyl acetate, and saturated n-butanol respectively, and each phase was extracted 10 times with 300 ml each time. The extracts were combined and dried by rotary evaporation. After the MTT cell screening test, it is calculated that the IC50 value of the ethyl acetate extraction phase in the ether phase, ethyl acetate phase, n-butanol phase, and water phase is the smallest, and the anti-tumor activity is high, so the ethyl acetate phase is selected for further subdivision .
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