Juglans mandshurica bark extract and application thereof in preparing anticancer drugs

A technology of walnut catalpa and its extract is applied to the bark extract of walnut catalpa and its application field in the preparation of anticancer drugs, which can solve the problem of complex composition of walnut catalpa bark, lack of systematic and in-depth exploration, and activity detection model only Limited to problems such as animals and tissues, the extraction and separation method is simple and easy to implement, enhances social productivity, and promotes the health of the social population.

Active Publication Date: 2013-09-04
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the components in the bark of Juglans catalpa are very complex, which ones play a key role in the obtained extract, which components have the main anticancer activity, and what are their exact anticancer effects? These work are lack of system and in-depth research
In addition, the anti-tumor research on Juglans catalpa mainly focuses on natural chemistry, and the applied activity detection models are limited to animals, tissues, etc.

Method used

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  • Juglans mandshurica bark extract and application thereof in preparing anticancer drugs
  • Juglans mandshurica bark extract and application thereof in preparing anticancer drugs
  • Juglans mandshurica bark extract and application thereof in preparing anticancer drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Take 500g of walnut catalpa bark (dry product), put it in a round bottom flask after crushing, add 4000ml of 70% ethanol, put it in an electric heating mantle and add a condenser tube to form a reflux system, repeat the reflux extraction for 3 times, the first 1000ml reflux for 1h, and then 3000ml was refluxed twice for 2.5h, and a total of 4000ml of ethanol solution with a mass fraction of 70% was used for reflux extraction for 3.5h. Use a rotary evaporator to recover ethanol under reduced pressure and concentrate the extract to a thick paste, add 5 times the amount of water to dissolve and disperse. After dissolving in water, use petroleum ether, ethyl acetate, and n-butanol for fractional extraction, and extract each phase 10 times, 300ml each time, for a total of 3000ml extract. The extract was distilled to dryness under reduced pressure to obtain petroleum ether components, ethyl acetate components, n-butanol components and water phase components respectively. Take ...

Embodiment 2

[0031] Take 500g of dried walnut catalpa bark, put it in a round-bottomed flask and add 5000ml of 70% ethanol to fully soak it, put it in an electric heating mantle and add a condenser tube to form a reflux system, and repeat the reflux extraction for 3 times, about 1700ml each time. About 1.5h, a total of reflux extraction 4.5h. Use a rotary evaporator to recover ethanol under reduced pressure and concentrate the extract to a thick paste, add 5 times the amount of water to dissolve and disperse. The solution was sequentially extracted with petroleum ether, ethyl acetate and n-butanol, 10 times each, and the extract was distilled to dryness under reduced pressure. The petroleum ether component, the ethyl acetate component, the n-butanol component and the water phase were respectively obtained. The anti-tumor cell activity test of above-mentioned four extraction phases, the results are shown in Table 1, the results show that the petroleum ether component, ethyl acetate compone...

Embodiment 3

[0036] Grind the bark of Catalpa walnut, take 500g of bark powder and extract 3 times with 70% ethanol under reflux, reflux with 4000ml for 3 hours for the first time, and reflux with 3000ml for 2.5h twice, use 7000ml of ethanol solution with a mass fraction of 70% for 5.5h in total . The extracts were combined, concentrated under reduced pressure, and the extract was collected. Dissolve with 5 times water, filter with suction, and save the filter residue. The filtrate was extracted with diethyl ether, ethyl acetate, and saturated n-butanol respectively, and each phase was extracted 10 times with 300 ml each time. The extracts were combined and dried by rotary evaporation. After the MTT cell screening test, it is calculated that the IC50 value of the ethyl acetate extraction phase in the ether phase, ethyl acetate phase, n-butanol phase, and water phase is the smallest, and the anti-tumor activity is high, so the ethyl acetate phase is selected for further subdivision .

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Abstract

The invention relates to a Juglans mandshurica bark extract with good anticancer effect, which is prepared by the following steps: (1) extracting the Juglans mandshurica bark with 70-75 wt% alcohol solution under reflux for 3.5-5.5 hours, evaporating to recycle alcohol so as to obtain a concentrated solution, dissolving by adding water into the concentrated solution, and extracting with ethyl acetate to obtain an liquid extract; and (2) after drying the liquid extract, separating by silicagel column chromatography, and eluting with an eluting agent to obtain an eluate, wherein the volume ratio of chloroform to methanol solution in the eluting agent is 3:2-0:1. The Juglans mandshurica bark extract which is extracted and separated in the invention has better effect than other existing Juglans mandshurica bark extracts in the aspect of cancer resistance, especially liver cancer resistance. The extraction and separation method of the Juglans mandshurica bark extract provided by the invention is simple and easy to implement.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to an extract from the bark of catalpa chinensis and its application in the preparation of anticancer drugs. Background technique [0002] The research and development of natural medicines play an important role in the process of finding new medicines and fighting diseases. Statistics show that among the 175 anticancer drugs used worldwide, about 57% are directly or indirectly derived from natural products. Therefore, the prospect of finding and developing new drugs from natural plants is very broad. my country has a vast territory and abundant natural medicine resources. How to make more effective use of these natural medicine resources and develop new medicines that can benefit mankind is an important task for drug researchers. The traditional natural medicine research is generally by first extracting, separating and identifying the chemical components in plants, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/52A61P35/00A61K129/00
Inventor 张咏莉陆家政李红枝黄清松崔玉强汪向升
Owner GUANGDONG PHARMA UNIV
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