Alkynidine glycosides and their extraction and separation methods and applications

A separation method and compound technology, applied in chemical instruments and methods, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of large solvent consumption, time-consuming, limited types, etc., and achieve the effect of rapid purification

Active Publication Date: 2019-11-08
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The types of existing alkyne glycosides are limited and the content is very low; and the existing extraction methods are time-consuming, consume a lot of solvents, and the recovery of solvents is cumbersome

Method used

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  • Alkynidine glycosides and their extraction and separation methods and applications
  • Alkynidine glycosides and their extraction and separation methods and applications
  • Alkynidine glycosides and their extraction and separation methods and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Take the platycodon powder that has passed a 200-mesh sieve, and then use 10, 8, and 6 times the amount of methanol to ultrasonically extract each time. The extraction time is 0.5h. After each extraction liquid is filtered, the extract is combined and concentrated under reduced pressure. The first extract; dissolve the first extract with distilled water, and then adsorb the obtained solution with AB-8 weakly polar macroporous resin, and use 8 column volumes of water and 30% ethanol for the first time Part of the sugar and inorganic salts in the solution are removed by elution, and then 50% ethanol is used for the second elution to enrich the polyacetylene components, and the eluate after the second elution is concentrated for the second time , Get the second extract; use countercurrent chromatography to use n-hexane: ethyl acetate: methanol: water (1:31:1:31, v / v / v / v) as the solvent system, flow rate 5mL / min, speed 800r / s, the second extract is separated and purified, a...

Embodiment 2

[0059] Take the powder of Platycodon grandiflorum that has passed a 300 mesh sieve and extract them with 9 and 7 times the amount of methanol ultrasonically. The extraction time is 0.7h each time. The extracted liquid is filtered and combined to obtain the extract, which is concentrated under reduced pressure. Extract: Dissolve the first extract with distilled water, and absorb the resulting solution with AB-8 weakly polar macroporous resin. First elution is carried out with a volume fraction of 40% ethanol to remove part of the sugar and part of the solution. Inorganic salts and other substances are eluted with 60% ethanol for a second time to enrich polyacetylene components, and the eluate after the second elution is concentrated for the second time to obtain the second extract; Chromatography uses n-hexane: ethyl acetate: methanol: water (1:28:1:32, v / v / v / v) as the solvent system, the flow rate is 4 mL / min, the rotation speed is 800 r / s, and the second extract is separated an...

Embodiment 3

[0061] Take the platycodon grandiflorum powder that has passed through a 250 mesh sieve, and then use 10, 8 times the amount of methanol to ultrasonically extract, each extraction time is 0.3h, after each extraction liquid is filtered, combined to obtain the extract, and concentrated under reduced pressure to obtain the first Extract: Dissolve the first extract with distilled water, and absorb the obtained solution with AB-8 weakly polar macroporous resin. First, use 20% ethanol for the first elution to remove part of the sugar and the solution. Inorganic salts and other substances are eluted with 55% ethanol for the second time to enrich polyacetylene components, and the eluate after the second elution is concentrated for the second time to obtain the second extract; Chromatography uses n-hexane: ethyl acetate: methanol: water (1:32:1:28, v / v / v / v) as the solvent system, the flow rate is 6 mL / min, the rotation speed is 800 r / s, and the second extract is separated and purified. ...

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Abstract

The invention relates to alkyne glycoside compounds and their extraction and separation method and application, belonging to the field of extraction and separation of natural compounds. The extraction and separation method is to ultrasonically extract the powder of Platycodon grandiflorum with methanol, and concentrate the extract to obtain the first extract; the first extract Add water to dissolve and add to the adsorption resin to carry out gradient elution with ethanol, and concentrate the eluent of gradient elution for the second time to obtain the second extract; the volume ratio of the second extract is 1:28-32:1:28 The solvent system of n-hexane, ethyl acetate, methanol and water of ‑32 is subjected to countercurrent chromatographic separation and purification, and the polyalkyne component effluent is collected; after the polyacetylene component effluent is concentrated for the third time, use high-performance liquid chromatography to obtain Acetonitrile-water is used as the mobile phase for preparation; the method is efficient and fast, and the purified alkyne glycoside compounds have antimicrobial, antitumor and other activities, and can be applied to the preparation of antibiotoxic drugs.

Description

Technical field [0001] The invention relates to the field of extraction and separation of natural compounds, and particularly relates to alkynyl glycoside compounds and methods and applications for extraction and separation thereof. Background technique [0002] Polyacetylene is a relatively special natural compound, mainly distributed in the plant tissues of Compositae, Campanulaceae, Araliaceae, Umbelliferae and Pittosporum spp. It has antimicrobial, cytotoxic and antitumor activities. And other aspects have good biological activity. The types of existing alkynyl glycoside compounds are limited and the content is very low; and the existing extraction method is time-consuming, solvent consumption is large, and solvent recovery is complicated. Summary of the invention [0003] The purpose of the present invention is to provide an alkynyl glycoside compound, which has good biological activity in terms of antimicrobial, cytotoxicity and antitumor activity, and can be applied to the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/10C07H1/08A61K31/7032A61P31/00A61P39/02A61P35/00
CPCC07H1/08C07H15/10
Inventor 刘政波陈宝
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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