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Three novel compounds in paeonia anomala and healthcare and medical applications of compounds

A compound, phenolic compound technology, applied in the field of neuroprotection and treatment of neurodegenerative diseases, can solve the problems of urgency in development and research on pharmacological activity

Inactive Publication Date: 2018-06-22
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are relatively few research reports on the chemical constituents and pharmacological activities of Paeoniae xinjiang. Therefore, it is urgent to study the chemical constituents, especially the development and pharmacological activities of new compounds in Paeoniae xinjiang.

Method used

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  • Three novel compounds in paeonia anomala and healthcare and medical applications of compounds
  • Three novel compounds in paeonia anomala and healthcare and medical applications of compounds
  • Three novel compounds in paeonia anomala and healthcare and medical applications of compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Extraction and separation of compounds

[0050] Get 10Kg of dried Radix Paeoniae Alba root, pulverize it and use 8 times the weight concentration of 80% ethanol to heat and reflux to extract 3 times, each time for 4 hours, after decompression and concentration to no alcohol smell, 2.5Kg of total extract is obtained, and the obtained The total extract is dispersed by adding water at a volume ratio of 1:9; the water dispersion is sequentially extracted 4 times with equal volumes of sherwood oil, ethyl acetate water-saturated n-butanol, and 450g of n-butanol part extract and Extraction of other parts; completely dissolve the extract of n-butanol part with 600ml of water, then use D101 macroporous resin as a carrier, load the sample by wet method, and elute with a water-ethanol solvent gradient of 0:100→100:0 to obtain Several eluted parts were detected by thin-layer chromatography, and the color was developed, and the eluted parts of each color were concentrated ...

Embodiment 2

[0051] Example 2 Extraction and separation of compounds

[0052]Get 10Kg of dried Radix Paeoniae Alba root, pulverize it and use 10 times the weight concentration of 80% ethanol to heat and reflux to extract 2 times, each time for 4 hours, and concentrate under reduced pressure until there is no alcohol smell to get 3.0Kg of total extract. The total extract is dispersed by adding water at a volume ratio of 1:12; the water dispersion is sequentially extracted 3 times with equal volumes of petroleum ether, ethyl acetate water-saturated n-butanol, and 500g of n-butanol part extract and Extraction of other parts: Dissolve the extract of n-butanol part completely with 650ml of water, then use D101 macroporous resin as a carrier, apply the sample by wet method, and elute with a water-ethanol solvent gradient of 0:100→100:0 to obtain Several eluted parts were detected by thin-layer chromatography, and the color was developed, and the eluted parts of each color were concentrated to dr...

Embodiment 3

[0053] Example 3 Extraction and separation of compounds

[0054] Take 10Kg of dry Xinjiang peony tuber root, pulverize it and use 10 times the weight concentration of 80% ethanol to heat and reflux extract 4 times, each time for 4 hours, after decompression and concentration to no alcohol smell, 2.8Kg of total extract is obtained, and the obtained The total extract is dispersed by adding water at a volume ratio of 1:6; the water dispersion is sequentially extracted with equal volumes of petroleum ether, ethyl acetate water-saturated n-butanol for 5 times, and the solvent is recovered to obtain 470g of n-butanol part extract and Other parts of the extract; the n-butanol part of the extract was completely dissolved in 630ml of water, then D101 macroporous resin was used as a carrier, and the sample was loaded by a wet method, and eluted with a water-ethanol solvent gradient of 0:100→100:0 to obtain Several eluted parts were detected by thin-layer chromatography, and the color wa...

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Abstract

The invention relates to the technical field of food and traditional Chinese medicines and provides a preparation method and applications of three novel compounds extracted and separated from paeoniaanomala. Chemical structural formulas of the compounds are shown in formulas (I), (II) and (III). Dried root tubers of paeonia anomala are crushed and then subjected to heating reflux extraction with80% ethanol, a total extract obtained through reduced pressure concentration is dispersed with water, extraction is performed with petroleum ether, ethyl acetate and water-saturated n-butyl alcohol with the same volume respectively, and parts are obtained. The n-butyl alcohol part is dissolved with water, macroporous resin column chromatography is performed, and fractions are obtained; the 30% ethanol part is separated and purified through silica gel column chromatography, a target chromatographic liquid is collected, separation with a C18 reversed phase chromatographic column and purificationwith Sephadex LH-20 (hydroxypropyl sephadex) are performed, and the compounds I, II and III are obtained. The invention also provides applications of the three compounds in neuroprotection and neurodegenerative disease treatment.

Description

technical field [0001] The invention relates to the technical fields of food and traditional Chinese medicine, in particular to three new compounds extracted, separated and identified from Xinjiang peony and their application in nerve protection and medicine for treating neurodegenerative diseases. Background technique [0002] Xinjiang peony Paeonia anomala subsp.anomala is a medicinal plant of Paeoniaceae Paeoniae genus Paeonia, which is distributed in Tacheng area of ​​Xinjiang. Because Xinjiang peony is rich in resources and wild, its roots are purchased in large quantities in Xinjiang, produced and sold by itself, and used as red peony in Uighur medicine and traditional Chinese medicine in Xinjiang. In the 2015 edition of "Pharmacopoeia of the People's Republic of China", Radix Paeoniae Rubra is the dried root of Paeonia lactiflora Pall. or Paeonia veitchii Lynch, a plant of the same genus. Radix Paeoniae Rubra is bitter in taste, slightly cold in nature, and returns t...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H17/04C07H1/08A61P25/00A61P25/28
CPCC07H1/08C07H15/203C07H17/04
Inventor 蒋建勤何文俊吕昊宇
Owner CHINA PHARM UNIV
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