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Method for extracting euscaphis konishii hayata high-efficiency anti-inflammatory action compound and product

A technology with anti-inflammatory effects of C. serrata, applied in the field of extraction of high-efficiency anti-inflammatory compounds of C. serrata, can solve the problem of water extract activity, extraction method and application field. No one has conducted research and no active substances. Or compound method reports, the wild crow tree has no problems such as artificial breeding and cultivation, and achieves the effect of low cost, small side effects and good effect.

Active Publication Date: 2015-01-14
福建药一制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the 8th issue of "Shizhen National Medicine and Chinese Medicine" in 2009, Li Xianhui et al. "Study on the Anti-inflammatory and Analgesic Effects of Wild Crow Extract" was published. In the 15th issue of 2013, "Contemporary Medicine" published Liu Didong et al. Effects of Chronic Liver Fibrosis in Rats”; According to related reports, the water extract of the leaves of S. japonica has a significant inhibitory effect on acute non-specific inflammation, and presents a dose-dependent effect, while for immune inflammation, the water extract of S. It has obvious inhibitory effect and has important practical application significance, but there is no report on the method of extracting active substances or compounds; at the same time, there is no large-scale artificial breeding and cultivation of Arvenia japonica, lack of resources, and lack of resource support for medicinal development
But no one has separated and studied its water extract, and no one has done research on the activity, extraction method and application field of the water extract, especially in terms of the anti-inflammatory effect of the water extract, there are no relevant reports in patents and literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take the pericarp of Coryda sinensis, dry, crush; condense and reflux extraction with distilled water for 3 times, filter for 3 hours each time, combine the filtrate, then extract with 3 times of n-butanol, extract 3 times, and combine the n-butanol extract , concentrated by rotary evaporation to obtain n-butanol extract S, which is a high-efficiency anti-inflammatory compound provided by the present invention. S yield is 36-40%.

[0017] Take S as the medicine, and convert according to the amount of folk medicine, 10-20g / adult, calculate and prepare the test medicine respectively.

[0018] The mouse ear swelling method was used to measure the inhibitory effect of the effector on inflammation; 340 male mice with a body weight of 18-22 g were selected and randomly divided into 34 groups with 10 mice in each group; each test drug was divided into large doses and small doses for testing. An equal volume of normal saline was used as the blank control, and dexamethasone was...

Embodiment 2

[0032] As in Example 1, the extract S was obtained from the stem of S. sylvatica as the material, and the anti-inflammatory effect was as in Example 1.

Embodiment 3

[0034] According to the method of Example 1, extract S was obtained from the root of Coryda sinensis as a material, and the anti-inflammatory effect was as in Example 1.

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PUM

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Abstract

The invention relates to a method for extracting a euscaphis konishii hayata high-efficiency anti-inflammatory action compound and a product. The method mainly comprises the following steps: taking a euscaphis konishii hayata plant, chopping, drying at the temperature of 50 DEG C and grinding; taking the plant powder, condensing and performing reflux extraction for three times by using distilled water, wherein the extraction time is 3 hours each time; filtering and merging so as to obtain the filtrate, extracting the filtrate by using n-butyl alcohol, extracting for three times, merging the n-butyl alcohol extracting solution, performing rotary evaporation and concentration, thereby obtaining the extract S, namely, the product provided by the invention. The extraction and separation method is simple and rich in resources, the extract is obvious in effects of diminishing inflammation and inhibiting capillary permeability, and the effects of the extract are obviously higher than those of dexamethasone injection.

Description

technical field [0001] The patent of the present invention belongs to the field of extraction method, product and application of plant active substances, and in particular relates to an extraction method and product of a high-efficiency anti-inflammatory compound of Coryda sinensis. Background technique [0002] Euscaphis is a plant of the genus Euscaphis in the oil family of the province. As a traditional Chinese herbal medicine in my country, it has a good medicinal foundation and a long history. It mainly treats stomach pain, bruises, rheumatic pain, and dizziness. , diarrhea, dysentery. In the 8th issue of "Shizhen National Medicine and Chinese Medicine" in 2009, Li Xianhui et al. "Study on the Anti-inflammatory and Analgesic Effects of Wild Crow Extract" was published. In the 15th issue of 2013, "Contemporary Medicine" published Liu Didong et al. Effects of Chronic Liver Fibrosis in Rats”; According to related reports, the water extract of the leaves of S. japonica has ...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P29/00
CPCA61K36/185A61K2236/331A61K2236/39A61K2236/51A61K2236/53
Inventor 邹双全邹芳芳刘宇
Owner 福建药一制药有限公司
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