Use of anti-inflammatory medicine for scheelite total saponin and its saponin compound

A technology of total saponins and compounds, applied in the application field of total saponins of Baiwei, Baiwei saponins, and drugs for the prevention and treatment of various acute and chronic inflammations, and can solve problems that have not yet been discovered

Inactive Publication Date: 2006-08-16
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, no literature reports have been found on the preparation of medicines from the total saponins of Baiwei and its monomeric saponins. The inventor has confirmed through a large number of experimental stud

Method used

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  • Use of anti-inflammatory medicine for scheelite total saponin and its saponin compound
  • Use of anti-inflammatory medicine for scheelite total saponin and its saponin compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Preparation of total saponins from Baiwei

[0060] After crushing 10 kg of dried root of Bai Wei, reflux 3 times with 10 times the amount of 90% ethanol for 2 hours each time, combine the extracts, recover the solvent under reduced pressure to obtain ethanol extract, and put the extract on a macroporous resin HP-20 column , respectively eluted with distilled water and 30% EtOH until the eluate was colorless, then eluted with 90% EtOH until the color of ethanol sulfate saponin was negative, recovered the 90% EtOH eluate to dryness, and obtained the 90% EtOH eluate , that is, the total saponin sample of Baiwei. Wherein, the content of myrtle saponin compound is more than 60%.

[0061] The total saponins of Baiwei, brown yellow powder, Lieberman-Burchard and Keller-Killiani reactions were all positive, indicating that they were all steroidal compounds containing 2-deoxysugar.

Embodiment 2

[0063] Preparation of Vinegrass A

[0064] After crushing 10 kg of dried root of Bai Wei, reflux 3 times with 10 times the amount of 90% ethanol for 2 hours each time, combine the extracts, recover the solvent under reduced pressure to obtain an ethanol extract, and put the extract on a macroporous resin HP-20 column. Elute with distilled water and 30% EtOH until the eluate is colorless, then elute with 90% EtOH until the color of ethanol sulfate saponin is negative, recover the 90% EtOH eluate to dryness, and obtain the 90% EtOH eluate fraction. The fraction eluted with 90% EtOH was subjected to normal-phase silica gel column chromatography, and gradient elution was carried out with chloroform-methanol, and the chromatographic fractions were checked by thin-layer chromatography. Fractions with the same single spot were combined, concentrated, and fractions were obtained 1, 2, 3, 4, 5, 3 and 4 of which were subjected to normal phase silica gel column chromatography, and eluted...

Embodiment 3

[0067] Preparation of ravine glycoside B

[0068] After crushing 10 kg of dried root of Bai Wei, reflux 3 times with 10 times the amount of 90% ethanol for 2 hours each time, combine the extracts, recover the solvent under reduced pressure to obtain an ethanol extract, and put the extract on a macroporous resin HP-20 column. Elute with distilled water and 30% EtOH until the eluate is colorless, then elute with 90% EtOH until the color of ethanol sulfate saponin is negative, recover the 90% EtOH eluate to dryness, and obtain the 90% EtOH eluate fraction. The fraction eluted with 90% EtOH was subjected to silica gel column chromatography, and gradient elution was carried out with chloroform-methanol alcohol, and the chromatographic fractions were checked by thin-layer chromatography. Fractions with the same single spot were combined and concentrated to obtain Fraction 1 , 2, 3, 4, 5, 3 and 4 of which were subjected to normal phase silica gel column chromatography, eluted with di...

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Abstract

Vincetoxin, its saponin compound and use in antiphlogistic medicine are disclosed. The vincetoxin consists of bine vincetoxin A, bine vincetoxin B, bine vincetoxin C, bine vincetoxin D, vincetoxicoside C-3-O-beta-D-thevetoside, vincetoxicoside C, vincetoxicoside H, atratoglaucosides A and cynanosides I. Vincetoxin is mixture which contains any kinds of the nine compounds with content >50%. It has excellent inhibiting function for various inflammations and can be used to treat chronic pharyngitis, chronic enterogastritis, acute tonsillitis and upper respiratory infection.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a class of compounds with anti-inflammatory activity, especially the saponin compound and total saponins of Baiwei extracted and separated from the traditional Chinese medicine Baiwei, and its role in the prevention and treatment of various acute and chronic inflammations. application in medicine. Background technique [0002] Baiwei is the root of Cynanchum atratum Bunge. and Cynanchum versicolor Bunge. in the family Asclepiadaceae. In Chinese medicine theory, Baiwei has the functions of clearing away heat and cooling blood, diuresis and treating stranguria, and detoxification. The effect of sores is used for warming evils and hurting the camp, fever due to yin deficiency, fever due to bone steaming, labor fever, postpartum blood deficiency fever, hot stranguria, bloody stranguria, carbuncle and swollen poison. In recent years, some pharmacological studies have bee...

Claims

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Application Information

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IPC IPC(8): C07J73/00A61K31/58A61P29/00A61K9/20A61K9/14A61K9/48A61K9/08
Inventor 张卫东郑兆广张川苏娟沈云亨柳润辉孔令义
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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