Radix asparagi steroid saponin extract and its preparing process and application

A kind of technology of steroidal saponins and extracts, applied in the field of total steroidal saponins extract of Asparagus and its preparation and application fields

Inactive Publication Date: 2005-01-26
SHANGHAI ZHENGXIANG NAUTRAL DRUG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report that the above-mentioned isolated compound has cardiovascular and cerebrovascular activity.

Method used

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  • Radix asparagi steroid saponin extract and its preparing process and application
  • Radix asparagi steroid saponin extract and its preparing process and application
  • Radix asparagi steroid saponin extract and its preparing process and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0254] Embodiment 1 n-butanol extraction method:

[0255] Asparagus coarse powder 1kg, extracted by percolation with 20 times the amount of 70-80% ethanol, and the ethanol was recovered under reduced pressure from the percolation liquid to obtain extract. The extract was diluted with an appropriate amount of water, and extracted twice with chloroform (500ml of chloroform each time). The aqueous solution extracted with chloroform was then extracted 4-5 times with n-butanol (400-500ml of n-butanol each time), the n-butanol extract was combined, the n-butanol was recovered under reduced pressure to dryness, and the residue was dissolved in a small amount of methanol. Add acetone under stirring until the precipitate no longer occurs, filter the precipitate under reduced pressure, and vacuum dry the precipitate to obtain a total steroidal saponin extract with a yield of about 4%, of which the furostanol saponin content is about 75%.

Embodiment 2

[0256] Embodiment 2 macroporous resin adsorption method:

[0257] Asparagus coarse powder 1kg, reflux extraction with 70~80% ethanol 3 times (each time with 1000ml of ethanol), the alcohol extract is recovered under reduced pressure until it has no alcohol smell, after diluting with water, put it on a D101 macroporous resin column for chromatographic separation, Sequentially elute with water, 20% ethanol, 70-80% ethanol, recover the solvent from the 70%-80% ethanol eluted part under reduced pressure to dryness, and vacuum dry the residue to obtain the total steroidal saponin extract, with a yield of about 5%, wherein furostanol saponin content is about 83%.

Embodiment 3

[0258] Example 3 Cerebrovascular Activity Test of Asparagus Total Steroidal Saponins Extract

[0259] 3.1 Purpose of the test: To observe the effects of asparagus total steroidal saponin extract, compound (1), and compound (3) on the cerebral blood vessels of anesthetized dogs.

[0260] 3.2 Test drugs: see Table 12.

[0261] 3.3 Animals: 27 healthy mongrel dogs, provided by the Experimental Animal Center of the Second Military Medical University, certificate number: SCXK (Shanghai) 2003-0002, weighing 13-15kg, both male and female, randomly divided into 5 groups as follows, see Table 12:

[0262] Table 12

[0263] Group Number of animals (only) Dose Administration route

[0264] Negative control group 6 Intravenous infusion of normal saline 5ml / kg

[0265] Nimodipine group 6 Nimodipine 300ug / kg intravenous infusion

[0266] Asparagus total steroidal saponins group 6 5mg / kg intravenous infusion

[0267] Compound (1) group 5 2.5mg / kg intravenous infu...

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Abstract

The invention provides a Radix asparagi steroid saponin extract and its preparing process and use thereof, wherein six compounds are isolated from the asparagi steroid saponin extract, among which four of them are novel compounds, the preparing process includes n-butyl alcohol extraction method and macroporous resin absorption method.

Description

technical field [0001] The present invention relates to active ingredient extracts obtained from natural medicines and their preparation methods and applications, in particular to the total steroidal saponin extracts obtained from the traditional Chinese medicine Asparagus, its preparation methods and its use in the preparation of cardiovascular and cerebrovascular drugs or food Applications. Background technique [0002] Asparagus cochinchinensis (Lour.) Merr., a traditional Chinese medicine, is the tuber root of Asparagus cochinchinensis (Lour.) Merr., which has the effects of nourishing yin and moistening dryness, clearing lung and reducing fire. Constipation, sore throat, etc. [State Administration of Traditional Chinese Medicine "Chinese Materia Medica" editorial board, ed. "Chinese Materia Medica"; Shanghai Science and Technology Press, 1999.8 (22): 63-69]. Asparagus is a commonly used traditional Chinese medicine and is rich in resources. TenjiK et al [Tenji K, et a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P9/00C07J71/00
Inventor 陈海生刘建国徐从立
Owner SHANGHAI ZHENGXIANG NAUTRAL DRUG TECH
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