Hydrophobic steroid saponin extract of peltate yam rhizome as well as preparation method and use thereof

A technology of steroidal saponins and extracts, which is applied in the field of turmeric hydrophobic steroidal saponins extracts, can solve the problems of turmeric hydrophobic steroidal saponins and no cancer, and achieve high-efficiency anti-cancer Active, stable drug effect, strong controllable effect

Inactive Publication Date: 2011-05-25
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on turmeric hydrophobic steroidal saponins, and there is no relevan

Method used

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  • Hydrophobic steroid saponin extract of peltate yam rhizome as well as preparation method and use thereof
  • Hydrophobic steroid saponin extract of peltate yam rhizome as well as preparation method and use thereof
  • Hydrophobic steroid saponin extract of peltate yam rhizome as well as preparation method and use thereof

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Experimental program
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Embodiment 1

[0036] The preparation of embodiment 1 medicine of the present invention

[0037] Clean the fresh tubers of Dioscorea baicalensis, cut into blocks, dry at 50°C, and pulverize. Weigh about 600g of turmeric powder into a 5000ml beaker, add about 2000ml of ethanol to soak overnight, filter with suction, separate the solid phase and liquid phase, transfer the solid phase to a 5L reflux bottle, add 1200ml-2000ml of ethanol, and reflux at 78°C for 2h-5h. Filtrate while hot, and repeat the reflux extraction 3 times. Combine the filtrate and the soaking liquid, distill under reduced pressure until there is basically no ethanol smell, reclaim the ethanol, add about 2000ml of water to ultrasonically dissolve the soluble saponin according to 6-10 times the weight of the extract, and centrifuge the suspension (4000-6000r / min, 10 -20min), separated the aqueous solution and the precipitate, and then freeze-dried the precipitate at -65°C for 36 hours to obtain 40g of hydrophobic steroidal t...

Embodiment 2

[0048] Embodiment 2 Drug HPLC fingerprint of the present invention

[0049] Chromatographic conditions: Shimadzu chromatograph LC-20; column: Shim-pack VP-ODS C 18 (250×4.6mm i.d.5um), pre-column (5μm, 10×4.6mm i.d.); binary mobile phase gradient elution, water (A) and acetonitrile (B), 0-5min, 20%-30%B; 5-35min, 30%-40%B; 35-40min, 30%-40%B; 40-60min, 60%-85%B; 60-75min, 85%-100%B; 75-80min, 100% B; flow rate: 1 ml / min; column temperature: 30° C.; detection wavelength: λ=204 nm; injection volume: 20 μl. Fingerprint see figure 1 , of which: 1. diosgenin, 2. trillium saponin, 3. diosgenin cellobioside, 4. diosgenin, 5. diosgenin, 6. triangle leaf saponin, 8. protocelloside, 9. original Diosgenin, 10. Prototriangin, 11. Dioscin

[0050] Table 1 Determination of each saponin content (mg / g) in the total hydrophobic saponins of Dioscorea scutellaria

[0051]

[0052] a Calculation formula: Saponin content = (cv) / m 0 . Among them: c: represents the concentration of the sa...

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Abstract

The invention provides a hydrophobic steroid saponin extract of peltate yam rhizome as well as a preparation method and use thereof. In the invention, in vitro anti-cancer drug experimental study proves that the hydrophobic steroid saponin extract of the peltate yam rhizome has strong growth inhibition activity on cell lines of colon cancer, lung cancer, breast cancer, cervical carcinoma, ovarian cancer, larynx cancer and the like. The extract of peltate yam rhizome has the advantages of wide resource, simple extraction and separation process of saponin, and wide prospect in cancer treatment, thus developing a new anticancer use of a hydrophobic dioscin component in the peltate yam rhizome.

Description

technical field [0001] The invention relates to a turmeric hydrophobic steroidal saponin extract, which belongs to the field of medicines. Background technique [0002] Tumor is a major disease that endangers human health and life. About 5 million people die of malignant tumors every year in the world. In recent years, due to the deterioration of the environment, as well as the work pressure of modern people, the spread of diseases, the incidence of malignant tumors is on the rise, and has become the second leading cause of death after cardiovascular diseases. Therefore, a large amount of manpower and material resources are invested in the research and development of new drugs to prevent and treat cancer every year. In recent years, in addition to studying western medicine, pharmacology experts have also conducted a lot of research on traditional Chinese medicine, and also explored the mechanism of malignant tumor formation. The invention and application of advanced techno...

Claims

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Application Information

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IPC IPC(8): A61K36/8945A61P35/00A61K125/00
Inventor 黄文吴晓华何杨卿勇邢志华李鹰
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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