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Solanum lyratum extract, and anticancer medicine comprising solanum lyratum extract

An extract, Baiying technology, applied in the field of medicine, can solve the problems of unclear and unstable active ingredients of Baiying extract, poor anti-tumor efficacy, etc.

Inactive Publication Date: 2012-01-18
XIYUAN HOSPITAL OF CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The purpose of the present invention is to provide a Baiying extract to solve the problem that the active ingredients of the Baiying extract in the prior art are not clear, the anti-tumor pharmacodynamic effect is good in vitro, but the overall anti-tumor efficacy in the body is not good or unstable technical issues

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  • Solanum lyratum extract, and anticancer medicine comprising solanum lyratum extract
  • Solanum lyratum extract, and anticancer medicine comprising solanum lyratum extract
  • Solanum lyratum extract, and anticancer medicine comprising solanum lyratum extract

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Embodiment 1

[0036] Preparation method: ethanol extraction: take the original medicinal material of Baiying (the dry whole herb of Solanaceae Baiying Solanum lyratum Thunb.), cut into sections, add 10 times the crude drug amount of 95% ethanol to soak overnight, reflux extraction for 3 hours, filter, and filter the residue with 10 70% ethanol twice as much as the drug was refluxed and extracted twice, each time for 3 hours, filtered, combined three extracts, recovered solvent was evaporated to dryness, and ethanol extract was obtained.

[0037] Refining: Take the ethanol extract, heat and dissolve it with 50 times the volume of water, and let it stand overnight. The supernatant is applied to a D151 macroporous resin column, rinsed with 95% ethanol, and then eluted with 6‰ hydrochloric acid ethanol. Collect 6‰ hydrochloric acid ethanol eluate, apply AB-8 macroporous resin for desalting, and concentrate the eluate to dryness to obtain refined Baiying extract.

Embodiment 2

[0039] Preparation method: ethanol extraction: get the original medicinal material of Baiying (Baiying is the dry whole herb of Solanum lytatum Thunb.), cut into sections, add 10 times the crude drug amount of 95% ethanol to soak overnight, reflux extraction for 3 hours, filter, The filter residue was reflux extracted twice with 70% ethanol with 10 times the amount of the crude drug, each time for 3 hours, filtered, the three extracts were combined, and the recovered solvent was evaporated to obtain the ethanol extract.

[0040] Refining: Take the ethanol extract, heat and dissolve it with 55 times the volume of water, and let it stand overnight. The supernatant is applied to a D151 macroporous resin column, rinsed with 95% ethanol, and then eluted with 6‰ hydrochloric acid ethanol. Collect 6‰ hydrochloric acid ethanol eluate, apply AB-8 macroporous resin for desalting, and concentrate the eluate to dryness to obtain refined Baiying extract.

Embodiment 3

[0042] Preparation method: ethanol extraction: get the original medicinal material of Baiying (Baiying is the dry whole herb of Solanum lytatum Thunb.), cut into sections, add 10 times the crude drug amount of 95% ethanol to soak overnight, reflux extraction for 3 hours, filter, The filter residue was reflux extracted twice with 70% ethanol with 10 times the amount of the crude drug, each time for 3 hours, filtered, the three extracts were combined, the recovered solvent was evaporated to dryness, and the ethanol extract was obtained.

[0043] Refining: Take the ethanol extract, heat and dissolve it with 60 times the volume of water, and let it stand overnight. The supernatant is applied to a D151 macroporous resin column, rinsed with 95% ethanol, and then eluted with 6‰ hydrochloric acid ethanol. Collect 6‰ hydrochloric acid ethanol eluate, apply AB-8 macroporous resin for desalting, and concentrate the eluate to dryness to obtain refined Baiying extract.

[0044] Water extra...

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Abstract

The invention discloses a solanum lyratum extract, and an anticancer medicine comprising the solanum lyratum extract. The solanum lyratum extract is prepared through steps that: a solanum lyratum medicinal material is extracted by using ethanol, wherein the solanum lyratum medicinal material is processed through ethanol refluxing extraction, the recovered solvent is evaporated until dried, such that an ethanol extract is obtained; the extract is refined, wherein the ethanol extract is dissolved in water to form a solution, the solution is absorbed by using a weak acidic cation exchange macroporous resin column and is orderly eluted by using ethanol and hydrochloric ethanol, the hydrochloric ethanol eluent is collected and is neutralized by using ammonia water, and the collected product isdesalted, such that the solanum lyratum extract is prepared. The solanum lyratum extract contains 1% to 60% of steroid alkaloid. According to the invention, weak acidic cation exchange macroporous resin is adopted, steroid alkaloid is specifically accumulated from various components of solanum lyratum. The component of accumulated steroid alkaloid is simple. As a result of experiments, the substance has a substantial anticancer potency, and the anticancer potency is greatly higher than that of solanum lyratum aqueous crude extracts and steroid saponins extracted by using n-butyl alcohol in prior arts.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an extract of bletilla baicalensis, its medical application in anticancer and antitumor aspects and anticancer drugs comprising the same. Background technique [0002] Baiying is the dry whole herb of Solanum lyratum Thunb., which is mainly produced in Zhejiang, Jiangsu, Anhui and other places. As a traditional Chinese medicine, it has been used for more than 2,000 years. It has the functions of clearing heat and detoxification, dispelling wind and phlegm, dispelling wind and dampness, and has anti-cancer effects. It is clinically used for the treatment of various cancers. [0003] The research on the chemical composition of Baiying began in the 1960s. So far, researchers have isolated more than 30 kinds of compounds from Baiying. The types of compounds mainly include steroidal saponins, steroidal alkaloids, organic acids, and Semiterpenes, sterols, coumarins and others. [0004] (1) St...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/81A61P35/00
Inventor 王建农
Owner XIYUAN HOSPITAL OF CHINA ACAD OF CHINESE MEDICAL SCI
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