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Application of cuckoopint steroid saponin effective component in preparation of drugs for preventing cerebral ischemia-reperfusion injury and its cerebrovascular disease sequel

A technology for trillium steroids and reperfusion injury, applied to medical preparations containing active ingredients, organic active ingredients, cardiovascular system diseases, etc., to achieve good market prospects, good therapeutic effects, and strong biological activity effects

Pending Publication Date: 2018-02-16
张忠立
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above studies have not been reported yet. Through systematic in vivo and in vitro experimental studies, we first found that the active ingredients of trillium steroidal saponins have a good therapeutic effect on cerebral ischemia-reperfusion injury and its sequelae of cerebrovascular diseases.

Method used

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  • Application of cuckoopint steroid saponin effective component in preparation of drugs for preventing cerebral ischemia-reperfusion injury and its cerebrovascular disease sequel
  • Application of cuckoopint steroid saponin effective component in preparation of drugs for preventing cerebral ischemia-reperfusion injury and its cerebrovascular disease sequel
  • Application of cuckoopint steroid saponin effective component in preparation of drugs for preventing cerebral ischemia-reperfusion injury and its cerebrovascular disease sequel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Trillium steroidal saponins extract and its active ingredient pinanogenin-3- O -α- L -rhamnopyranosyl(1→2)-β- D -Glucoside, pinanogenin-3- O -α- L -Rhamnopyranosyl(1→4)-[α- L -Rhamnopyranosyl(1→2)]-β- D -Glucoside and Pinogenin-3- O -α- L -rhamnopyranosyl(1→4)-α- L -Rhamnopyranosyl(1→4)-[α- L -Rhamnopyranosyl(1→2)]-β- D -Preparation method of glucoside: grind Trillium herbaceae into coarse powder, soak in 70% ethanol for 1 hour, heat and reflux extraction with 10 times the amount of solvent for 3 times, each time for 2 hours, filter, and combine the filtrates. The above filtrate was concentrated under reduced pressure at 65°C to 1.0g raw medicinal materials / ml, refrigerated overnight at 0-4°C, gelatinized, filtered, and the filtrate was extracted with an organic solvent such as n-butanol, and the organic solvent was recovered to obtain the extract and suspended in water. Enriched with AB-8 macroporous adsorption resin, eluted with water, 30% ethanol, 60% ethan...

Embodiment 2

[0027] Trillium steroidal saponins extract and its active ingredient pinanogenin-3- O -α- L -rhamnopyranosyl(1→2)-β- D -Glucoside, pinanogenin-3- O -α- L -Rhamnopyranosyl(1→4)-[α- L -Rhamnopyranosyl(1→2)]-β- D -Glucoside and Pinogenin-3- O -α- L -rhamnopyranosyl(1→4)-α- L -Rhamnopyranosyl(1→4)-[α- L -Rhamnopyranosyl(1→2)]-β- D -Preparation method of glucoside: grind trillium herbaceous material into coarse powder, soak in 60% ethanol for 2.0 hours, heat and reflux extraction with 12 times the amount of solvent for 3 times, each time for 2 hours, filter, and combine the filtrates. The above filtrate was concentrated under reduced pressure at 70°C to 1g raw medicinal materials / ml, refrigerated at 0-4°C overnight, gelatinized, filtered, enriched with D101 macroporous adsorption resin on the filtrate, followed by water, 30% ethanol, 60% ethanol and 85 % ethanol to obtain the eluted parts, dry by spray drying method, and collect the spray dry powder to obtain the final pr...

Embodiment 3

[0029] Trillium steroidal saponins extract and its active ingredient pinanogenin-3- O -α- L -rhamnopyranosyl(1→2)-β- D -Glucoside, pinanogenin-3- O -α- L -Rhamnopyranosyl(1→4)-[α- L -Rhamnopyranosyl(1→2)]-β- D -Glucoside and Pinogenin-3- O -α- L -rhamnopyranosyl(1→4)-α- L -Rhamnopyranosyl(1→4)-[α- L -Rhamnopyranosyl(1→2)]-β- D -Preparation method of glucoside: grind trillium herbaceous material into coarse powder, soak in 50% ethanol for 1.0 hour, heat and reflux extraction with 8 times the amount of solvent for 3 times, each time for 2 hours, filter, and combine the filtrates. The above filtrate was concentrated under reduced pressure at 70°C to 1g raw medicinal materials / ml, refrigerated overnight at 0-4°C, gelatinized, filtered, enriched with HPD400 macroporous adsorption resin on the filtrate, water, 30% ethanol, 50% ethanol and 85 % ethanol to obtain the eluted parts, dry by spray drying method, and collect the spray dry powder to obtain the final product. Amon...

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Abstract

The invention discloses an application of a cuckoopint steroid saponin effective component in preparation of drugs for preventing cerebral ischemia-reperfusion injury and its cerebrovascular disease sequel. The prepared cuckoopint steroid saponin effective component is one or more of pennogenin, pennogenin-3-O-alpha-L-rhamnopy ranosyl (1->2)-beta-D-glucoside, pennogenin-3-O-alpha-L-rhamnopy ranosyl (1->4)-[alpha-L- rhamnopy ranosyl(1->2)-beta-D-glucoside and pennogenin-3-O-alpha-L-rhamnopy ranosyl (1->4)-alpha-L- rhamnopy ranosyl(1->4)- alpha-L-rhamnopy ranosyl (1->2)-beta-D-glucoside. For thecuckoopint steroid saponin effective component has the common structure of pennogenin-3-O-alpha-L-rhamnopy ranosyl (1->2)-beta-D-glucoside, the composition has the similar treatment effect in a human body; the composition is mainly applied to the clinical drugs for treating the cerebral hemorrhage or cerebral ischemia-reperfusion injury and its cerebrovascular disease sequel caused by cardiocerebral vascular system disease.

Description

technical field [0001] The invention relates to the application of active ingredients of trillium steroidal saponins in the preparation of anti-cerebral ischemia-reperfusion injury and its sequelae of cerebrovascular disease, and belongs to the technical field of traditional Chinese medicine preparation. Background technique [0002] Trillium is a plant of the genus Trillium in the family Liliaceae Trillium tschonoskii Maxim., commonly known as a bead on the top of the head, taro seven, lion seven, etc., is a traditional Chinese medicine with the effect of prolonging life. Indications of dizziness, insomnia, bruises, traumatic bleeding, neurasthenia, hypertension, sequelae of concussion and other diseases, is one of the famous folk medicines of Enshi Tujia. At present, domestic and foreign studies on the pharmacological activity of Trillium plants have shown that Trillium has strong anti-inflammatory, immune regulation, improving learning and memory functions of rats, and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61K31/7048A61P9/10A61P25/00
CPCA61K31/7048A61K36/896A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 张忠立左月明
Owner 张忠立
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