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Tribulus total steroid saponin and preparation thereof

A technology of steroidal saponins and total spirosteroidal saponins, which is applied in the field of saponins, can solve problems such as troublesome preparation process and unsuitability for industrialized large-scale production, and achieve the effects of easy-to-obtain production equipment, simple preparation method, and easy-to-master production technology

Inactive Publication Date: 2008-09-10
JIANGXI XINYUANJIAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another example is "A Refined Tribulus Terrestris Saponin Available for Injection Administration, Preparation Method and Injection" disclosed by the publication number CN1631420A. Although the content of total saponins of Tribulus terrestris is very high, the preparation process is complicated and cumbersome, and it is not suitable for industrialized large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0043] Embodiment 1 The preparation method of the first total steroidal saponins of Tribulus terrestris

[0044] Take 1 kg of Tribulus terrestris, reflux extraction with 4 times the amount of 60% ethanol for 2 times (1 hour / time), combine the extracts, filter, concentrate the filtrate until it has no alcohol smell, and adjust the volume of the concentrated solution to the equivalent of each ml of the medicinal solution Refrigerate 1 gram of crude drug at 0°C for 10 hours, centrifuge and filter, and put the filtrate on a D101 macroporous resin column; after loading it on the column, make it fully adsorbed, wash it with water until it is colorless, and the reducing sugar reaction is negative , then washed with 20% ethanol until colorless, then eluted with 80% ethanol, collected the eluent, recovered the ethanol to the utmost, concentrated under reduced pressure, dried, and pulverized to obtain the total steroidal saponins of Tribulus terrestris, which was determined by the aforem...

specific Embodiment approach 2

[0045] Specific embodiment two The preparation method of the second total steroidal saponins of Tribulus terrestris

[0046] Take 1 kg of Tribulus terrestris, reflux extraction with 5 times the amount of 70% ethanol for 2 times (2 hours / time), combine the extracts, filter, concentrate the filtrate until it has no alcohol smell, and adjust the volume of the concentrated solution to the equivalent of each ml of the medicinal solution Refrigerate 2 grams of crude drug at 5°C for 12 hours, centrifuge and filter, and put the filtrate on an AB-8 macroporous resin column; If it is negative, wash with 10% ethanol until it is colorless, then elute with 70% ethanol, collect the eluent, recover the ethanol to the utmost, concentrate, dry under normal pressure, and pulverize to obtain the total steroidal saponins of Tribulus terrestris. Determination, containing furostanol saponin is 59.3%.

specific Embodiment approach 3

[0047] Specific embodiment three The preparation method of the third total steroidal saponins of Tribulus terrestris

[0048]Take 1 kg of Tribulus terrestris, reflux extraction with 6 times the amount of 80% ethanol for 2 times (3 hours / time), combine the extracts, filter, concentrate the filtrate until it has no alcohol smell, and adjust the volume of the concentrated solution to be equal to that of each ml of the medicinal solution. Refrigerate 3 grams of crude drug at 1°C for 12 hours, centrifuge and filter, and put the filtrate on a NKA-9 macroporous resin column; When negative, wash with 15% ethanol until it is colorless, then elute with 75% ethanol, collect the eluate, recover the ethanol to the utmost, concentrate, and spray dry to obtain the total steroidal saponins of Tribulus terrestris. The furostanol saponin is 63.5%.

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Abstract

The present invention discloses a caltrop total steroid saponin which comprises caltrop total spirostanol saponin and caltrop total furostanol saponin and is characterized in that the content of the caltrop total furostanol saponin is more than 50 percent (W / W), which can meet the requirement of developing more than five kinds of new traditional Chinese medicines. The present invention also discloses a preparation method for the caltrop total steroid saponin, which has the following steps of (1) extracting ethanol; (2) filtering and concentrating; (3) absorbing macroporous resin; (4) washing pillar; (5) concentrating; (6) drying; (7) crushing, and the caltrop total steroid saponin can be obtained. The macroporous resin absorption means absorbing and concentrating the macroporous resin having the aperture of 90A degrees to 100A degrees and the specific surface area of 500m<2> / g to 550 m<2> / g above the filtrate of concentrated solution to obtain a midbody. The preparation method is simple; corresponding production equipments can be obtained easily; the production technology is grasped easily, which is especially suitable for industrialized mass production. The present invention collects the eluate with the ethanol having corresponding concentration by one step alcohol extracting-water precipitating and then concentrating and absorbing the macroporous resin for condensing, drying and crushing to obtain the caltrop total furostanol saponin.

Description

technical field [0001] The invention relates to saponins, in particular to a total steroidal saponin from Tribulus terrestris and a preparation method thereof. Background technique [0002] Tribulus terrestris is the dried and mature fruit of Tribulus terrestris L., a plant of the family Tribulus terrestris. Contains chemical components such as saponins, alkaloids, flavonoids, etc. It is pungent, bitter, slightly warm in nature, slightly poisonous, and returns to the liver meridian; It is often used in the treatment of headache, dizziness, chest and hypochondriac pain, mastitis, red eyes and nebula. [0003] The total saponins of Tribulus terrestris are total steroidal saponins, including two types of total spirostanoid saponins and total furostanoid saponins, and the content of furostanol saponins in Tribulus terrestris total steroidal saponins obtained by different preparation methods is different. Chinese patent CN1314402C announced "medicine composition containing tota...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P1/16A61P15/14A61P27/02A61P1/08A61K131/00
Inventor 漆小燕漆小勇曾建伟
Owner JIANGXI XINYUANJIAN PHARMA
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