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Method of preparing allium macrostemon total sterides saponin extract and uses thereof

A technology of extracts and total steroids, which is applied in the field of preparation of drugs for the prevention and treatment of myocardial ischemia, can solve the problems of low purity, long cycle, dark color of total steroidal saponins, etc., and achieve high purity, low production cost, and preparation The effect of simple process

Inactive Publication Date: 2008-08-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adopt this method technological process cycle to be longer, and the total steroidal saponin that obtains is dark in color, and purity is lower

Method used

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  • Method of preparing allium macrostemon total sterides saponin extract and uses thereof
  • Method of preparing allium macrostemon total sterides saponin extract and uses thereof
  • Method of preparing allium macrostemon total sterides saponin extract and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1. Preparation of total saponins of Allium scallion

[0022] Take 1 kg of Allium scallion bulbs, cut them into small pieces of 1-3 cm, add 5 L of 95% ethanol to reflux and extract twice, until the color of the extract is lighter. Combine the extracts, concentrate under reduced pressure to 100ml, add 800ml of distilled water and let stand, take the lower sediment and dilute with 150ml of water, extract three times with water-saturated n-butanol, backwash the n-butanol extract once with water and concentrate under reduced pressure To dryness, the residue was dissolved in 100ml of 95% ethanol, 500ml of acetone was added until no precipitation occurred, filtered, and dried to obtain 4.6g of the total steroidal saponin extract of Scallopsia officinalis, with a yield of 0.46%.

[0023] The total steroidal saponin extract of Scallopia sativa obtained by the above method is nearly white. In the present invention, compound 1 is used as a standard product, and the con...

Embodiment 2

[0024] Example 2. Pilot-scale preparation of total saponins from Allium scallion

[0025] Take 30kg of allium bulbs, cut them into small pieces with a knife, add 150 L of 50% ethanol, and extract at room temperature for 3 times, each time for 48 hours. The extracts were combined, concentrated to 30L, and the precipitate was collected by filtration. Add 3.0 L of double distilled water to disperse. Extract four times with water-saturated n-butanol, backwash the extract once with redistilled water, then concentrate to dryness under reduced pressure, add 1.0L 95% ethanol to completely dissolve, filter, add 3.0L acetone to the filtrate, and immediately see white Powdery precipitate appeared, filtered with suction, and the filtrate was concentrated to 0.5L. Add 2.0 L of acetone again to the filtrate, and filter with suction. The filter residues obtained by the two suction filtrations were combined to obtain 95.0 g of refined total steroidal saponins, with a yield of 0.32%.

Embodiment 3

[0026] Example 3. Study on the pharmacological activity of total saponins of Allium scallion

[0027] 1. Experimental method: cardiomyocytes were divided into 3×10 5 The cells / ml density was seeded in a 96-well plate (200 μl per well), at 37°C, 5% CO 2 Under the condition, the culture medium is cultured in DMEM containing 10% fetal bovine serum. When the cells grow to the sub-confluent state, add the preparation of total steroidal saponins of S. scallops with a final concentration of culture medium, cultivated for 48h. Add 0.5mmol / L H 2 o 2 Injury for 10 minutes, suck out the culture medium of each group, wash with Hanks solution for 3 times, then add DMEM culture medium containing 10% fetal bovine serum, at 37°C, 5% CO 2 Continue culturing for 24 hours under the same conditions, then add MTT (final concentration: 0.5mg / ml) and cultivate for 4 hours, suck out the culture solution containing MTT, add 100 μl DMSO to each well, and after the particles in the culture wells ar...

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Abstract

The invention relates to a preparation method of the extraction of total steroid saponins of longstamen onion bulb, which is as follow: cutting the longstamen onion bulb into pieces; adding ethanol for lixiviation or reflux extraction; combining extraction liquid; decompression and concentration; quiescence or centrifugation; taking and diluting the lower sediment with water; extracting with water saturated normal butanol and then decompressing and concentrating until no liquid; dissolving the residue with ethanol and adding acetone until no production of sediment; filtering and drying to obtain the extraction of total steroid saponins. The preparation method has the advantages of rational design, simple technology, low production cost, high yield, high purity of product. The primary study of biological activities shows that the extraction of total steroid saponins has obvious protection effect for the myocardial cell trauma induced by H2O2, and can be used for the preparation of medicines with obvious protection effect for myocardial cell trauma.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for preparing total steroidal saponins of Allium scallops from Chinese medicinal material Allium scallops and its application in the preparation of drugs for preventing and treating myocardial ischemia. Background technique [0002] Allium macrostemon or Allium chinensis is the bulb of Allium macrostemon or Allium chinensis in the family Liliaceae. It is mainly produced in China and has been widely cultivated as a medicinal and edible plant for thousands of years. Allium scallion is a traditional Chinese medicine in my country. It tastes bitter, pungent, warm in nature, and non-toxic. Traditional medicine believes that it has the effects of regulating qi, broadening the chest, unblocking yang and dispelling stagnation. Modern studies have shown that allium has physiological effects such as lowering blood pressure, lowering blood sugar, preventing atheroscle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8962A61K9/12A61K9/20A61K9/48A61P9/10C07J71/00A61K125/00
Inventor 周长新吴理茂李连达任刚
Owner ZHEJIANG UNIV
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