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Method for obtaining ferulic acid by using wastes

A technology for ferulic acid and waste, applied in the field of obtaining ferulic acid, can solve the problems of immature process, difficult separation, long production cycle, etc., and achieve the effects of less capital investment, stable reaction process and simple production equipment

Inactive Publication Date: 2018-12-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical synthesis method has a long production cycle, but it will produce a mixture of cis and trans ferulic acid, with complex components and difficult subsequent separation
Biosynthesis is efficient and clean, but the process is not yet mature

Method used

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  • Method for obtaining ferulic acid by using wastes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In this example, the mixing ratio of distiller's grains and bran is 1:1, the cultivation temperature is 30°C, and the extraction temperature of ferulic acid in the distiller's grains solid-state fermentation product is extracted by hot water extraction method is 60°C;

[0023] (1) Preparation of Cladosporium spore suspension

[0024] Streak-inoculate the Cladosporium spores stored in glycerol tubes on PDA solid medium, culture at 30°C for 3 days, then add 5mL sterile saline to each plate to wash the spores generated by the mycelium, and make a spore suspension .

[0025] (2) Seed medium and cultivation

[0026] The seed medium of Cladosporium cladoides is a liquid PDA medium, and the liquid volume in 250mL is 100mL and 10 glass beads are added. After sterilization, 3 mL of the spore suspension in step (1) was inserted, and the spore density was 1×10 6 / mL, and cultured at 30° C. and 150 rpm for 5 days.

[0027] (3) Solid-state fermentation of distiller's grains

[0...

Embodiment 2

[0032] In this example, the mixing ratio of distiller's grains and bran is 1:1, the cultivation temperature is 30°C, and the extraction temperature of ferulic acid in the distiller's grains solid-state fermentation product is extracted by hot water extraction method is 60°C;

[0033] (1) Preparation of Cladosporium spore suspension

[0034] Streak-inoculate the Cladosporium spores stored in glycerol tubes on PDA solid medium, culture at 30°C for 3 days, then add 5mL sterile saline to each plate to wash the spores generated by the mycelium, and make a spore suspension .

[0035] (2) Seed medium and cultivation

[0036] The seed medium of Cladosporium cladoides is a liquid PDA medium, and the liquid volume in 250mL is 100mL and 10 glass beads are added. After sterilization, 3 mL of the spore suspension in step (1) was inserted, and the spore density was 1×10 6 / mL, and cultured at 30° C. and 150 rpm for 4 days.

[0037] (3) Solid-state fermentation of distiller's grains

[0...

Embodiment 3

[0042] In this example, the mixing ratio of distiller's grains and bran is 1:1, the cultivation temperature is 30°C, and the extraction temperature of ferulic acid in the distiller's grains solid-state fermentation product is extracted by hot water extraction method is 60°C;

[0043] (1) Preparation of Cladosporium spore suspension

[0044] Streak-inoculate the Cladosporium spores stored in glycerol tubes on PDA solid medium, culture at 30°C for 3 days, then add 5mL sterile saline to each plate to wash the spores generated by the mycelium, and make a spore suspension .

[0045] (2) Seed medium and cultivation

[0046] The seed medium of Cladosporium cladoides is a liquid PDA medium, and the liquid volume in 250mL is 100mL and 10 glass beads are added. After sterilization, 3 mL of the spore suspension in step (1) was inserted, and the spore density was 1×10 6 / mL, and cultured at 30° C. and 150 rpm for 8 days.

[0047] (3) Solid-state fermentation of distiller's grains

[0...

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Abstract

The invention discloses a method for obtaining ferulic acid by using wastes, and belongs to the technical field of the fermentation industry. The method comprises the following steps: mixing of distiller's grains with the bran, inoculation, cultivation for obtaining a solid fermentation product of the distiller's grains, and extraction of the ferulic acid in the solid fermentation product of the distiller's grains by a hot water extraction process. The wastes bran and the distiller's grains in the grain processing and wine brewing processes are used as raw materials, so the cost and the pollution are reduced, and the added values are increased; the ferulic acid is enriched by using Cladosporium cladosporioides, so a large class of bacteria is provided for the enrichment production of the ferulic acid to select; and further, the method has the advantages of simple fermentation production device, low capital investment, stable reaction process, convenience in implementation, and facilitation of industrial production.

Description

technical field [0001] The invention relates to a method for obtaining ferulic acid from waste, and belongs to the technical field of fermentation industry. Background technique [0002] Ferulic acid was originally found in the seeds and leaves of plants. It is a phenolic acid widely present in plants. It combines with polysaccharides and proteins in the cell wall to form the skeleton of the cell wall, and rarely exists in a free form. Studies have shown that ferulic acid is one of the active ingredients of many traditional Chinese medicines such as Ferulicum, Chuanxiong, Angelica, Cimicifuga, etc., and is widely present in agricultural by-products such as corn bran and rice bran. Ferulic acid and its derivatives have various biological activities, such as anti-platelet aggregation, promoting platelet depolymerization, lowering blood lipid, anti-thrombotic, anti-oxidation, anti-free radical, anti-inflammatory, anti-tumor, anti-mutation, enhancing immune function and Enhance...

Claims

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Application Information

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IPC IPC(8): C12P7/42C12R1/645
CPCC12P7/42
Inventor 毛健彭林刘双平姬中伟韩笑艾斯卡尔·艾拉提朱蕊
Owner JIANGNAN UNIV
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