Probe and kit for common pathogens detection of skin infectious granuloma

A pathogen detection and skin infection technology, which is applied in the measurement/test of microorganisms, resistance to vector-borne diseases, DNA/RNA fragments, etc., can solve the problems of cumbersome and scattered detection processes, improve clinical diagnosis and treatment efficiency, and improve prognosis , the effect of rapid diagnosis

Inactive Publication Date: 2013-11-27
王洪生 +1
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Problems solved by technology

However, the above-mentioned detectio

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  • Probe and kit for common pathogens detection of skin infectious granuloma
  • Probe and kit for common pathogens detection of skin infectious granuloma
  • Probe and kit for common pathogens detection of skin infectious granuloma

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Experimental program
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Embodiment Construction

[0024] 1. The composition of the kit

[0025] 1. 96-well ELISA plate

[0026] The ELISA plate was purchased from Costar 96-well plate coated with carboxyl groups.

[0027] 2. Design common primers for each strain

[0028] Cultivate common pathogenic bacteria causing skin infectious granuloma: 6 kinds of mycobacteria (Mycobacterium tuberculosis, Mycobacterium leprae, Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium kansasii and Mycobacterium fortuitously), 5 kinds Fungi (Candida albicans, Sporothrix schenckii, Cladosporium carinii, Chromobacter pei, Fonsecaea monophora), Nocardia, Actinomycetes and Leishmania.

[0029]Select 5 pairs of primers: mycobacterial universal primer (upstream primer 5'-ACCAACGATGGTGTG TCCAT-3', downstream primer 5'-CTTGTCGAACCGCATACCCT-3'), fungal universal primer (upstream primer 5'-TCCGTAGGTGAACCTGCGG-3', downstream primer 5 '-TCCTCCGCTTATTGATATGC-3'), Nocardia (upstream primer 5'-CTCCCATATGGTCGACCT-3', downstream primer 5'-TCACGAC...

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Abstract

The invention discloses a probe and a kit for the common pathogens detection of skin infectious granuloma. The probe comprises a mycobacterium probe, a mycobacterium tuberculosis probe, a mycobacterium leprae probe, a mycobacterium avium probe, a mycobacterium intracellulare probe, a mycobacterium kansasii probe and a mycobacterium fortuitum probe, a candida albicans probe, a sporotrichum schenckii probe, a cladosporium trichoides probe, a Fonsecaea pedrosoi probe, and a Fonsecaea monophora probe; and a nocardia probe, an actinomycetes probe and a leishmania probe. The nucleotide sequence of each probe is shown in sequence tables SEQ ID NO.1-SEQ ID NO.15; and the kit comprises the probes and corresponding universal primers. According to the invention, through designing and optimizing the kit for carrying out pathogens screening and detection on common skin infectious granuloma, the improvement of the clinical diagnosis and the treatment efficiency is facilitated.

Description

technical field [0001] The invention relates to a probe and a kit for detecting common pathogens of skin infectious granuloma. Background technique [0002] Infectious granulomatosis of the skin is a group of diseases mainly caused by pathogens such as fungi, mycobacteria, actinomycetes, Nocardia and Leishmania, and the histopathological changes are granulomatous. In my country, fungi and mycobacteria are the main pathogenic bacteria, which can not only cause skin and subcutaneous tissue infection, but also lead to life-threatening systemic infection through hematogenous dissemination or direct infiltration and diffusion. Chromoblastomycosis, phaeohyphomycosis, sporotrichosis and other biphasic fungal infections caused by various fungi are becoming more and more common in dermatology clinics; tuberculosis ranks first among human fatal infectious diseases, and skin tuberculosis such as lupus vulgaris It can cause disfigurement, and leprosy can cause teratogenicity, disabilit...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCY02A50/30
Inventor 王洪生刘维达闫桢桢姜海琴
Owner 王洪生
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