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Application of licorice root extract

A licorice extract and extract technology, applied in the field of new application of licorice extract in the prevention and treatment of plant diseases, can solve the problems that the antibacterial activity has not been reported, and achieve the effect of solving the problem of resistance

Inactive Publication Date: 2009-09-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The bactericidal activity of licorice can be found in medical reports, but there is no report about the bacteriostatic activity of licorice and its extracts on plant pathogenic bacteria.

Method used

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  • Application of licorice root extract
  • Application of licorice root extract
  • Application of licorice root extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation of embodiment 1 licorice extract

[0019] The roots of licorice were collected and dried in a 50°C electric constant temperature blast drying oven; pulverized by a pulverizer and passed through a 40-mesh sieve. The obtained plant powder is sealed in a sealed bag and stored in a dark place for extraction.

[0020] The present invention adopts the existing conventional ultrasonic extraction method to prepare the licorice extract, and can also refer to other existing conventional techniques for extraction and preparation.

Embodiment 2

[0021] Embodiment 2: The inhibitory effect of licorice methanol crude extract on Peronophythora Chen (Peronophythora Chen) pathogens

[0022] 75% methanol was used as solvent to dissolve the extract, and then mixed with PDA medium to prepare a poisonous medium, and the inhibitory effect of different solvent extracts on the mycelial growth of Ponosophthora litchie was determined by the method of mycelial growth rate. The specific operation is:

[0023] Mycelia growth rate method: use 75% methanol as a solvent to prepare the licorice extract into a certain concentration of the test drug solution, accurately draw 1mL of the drug solution and add it to 49mL of melted PDA medium (about 50°C), mix evenly and pour Put it into a sterilized petri dish (diameter 9cm), pour each bottle evenly into 3 petri dishes, and prepare the poisonous culture medium with the required concentration. The control was mixed with the same volume of sterile water or 75% methanol. The test pathogen Pythor...

Embodiment 3

[0030] Embodiment 3: The inhibitory action of the crude extract of licorice methanol to Peronophythora Chen (Peronophythora Chen) pathogens

[0031] Adopt the glass slide method (Fang Zhongda, 1998) to measure: the sporangia that will be cultivated on the PDA substratum for 10 days with aseptic water to wash the sporangia of Peronospora litchie, add medicinal liquid to be prepared into spores containing various concentrations of medicinal liquid For the suspension of sporangia, adjust the concentration of sporangia to about 40-60 sporangia per field of view (10×10 times), take 50 μL of the sporangia suspension and drop it on the slide, and then place the slide on a place where a piece of filter paper has been placed. In a 9cm petri dish, a small amount of sterile water was added dropwise on the filter paper to keep it moist, and the petri dish was covered, and cultured under light at 28°C for 4 hours to observe the sporangia germination. The sporangia germination rate and the ...

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Abstract

The invention discloses an application of a licorice root extract, which is particularly applied to controlling plant diseases. By indoor bioassay and pot experiment, the invention provides a good control application of the methyl alcohol extract of a licorice root for common plant diseases to peronophythora, phytophthora, cladosporium, sclerotinia and colletotrichum, and the like. The invention proposes a new concept for the licorice root and the extract thereof used for controlling plant diseases, provides a new exploitative resource for plants to replace chemosynthetic fungicide, and has an important significance for solving the growing resistibility problem of pathogenic bacteria to medicine.

Description

technical field [0001] The invention belongs to the technical field of plant fungicides, and in particular relates to a new application of a licorice extract in preventing and treating plant diseases. Background technique [0002] In recent years, with the continuous development of phytopathology, biology and other related disciplines, the research on fungicides in pesticides has entered a new period. The emergence of high-efficiency systemic fungicides such as Strobilurn fungicides, on the one hand, has made a breakthrough in the prevention and control of plant diseases, and on the other hand, it has also caused the problem of pathogenic bacteria's resistance to the fungicides to become increasingly prominent. Seeking pollution-free pesticides with good environmental compatibility and long-lasting effects on pathogenic bacteria is particularly important today when green food is increasingly valued. [0003] Natural products often exhibit many biological activities and have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/20A01P3/00
Inventor 罗建军胡美英钟国华郝卫宁何玥胡黎明
Owner SOUTH CHINA AGRI UNIV
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