Application of Penicillium Polish extract in preparation of herbicide
A technology of extracts and herbicides, applied in the field of biological control, can solve problems such as environmental pollution, herbicide dosage, crop injury risk, and increase the cost of weed control, achieve high weeding efficiency, wide application value, and solve weeds The effect of resistance problems
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Embodiment 1
[0028] Example 1. Preparation of Penicillium Polonia Extract
[0029] Respectively activate the strain of Penicillium polandum No. 1 (preservation unit: China Agricultural Microorganism Culture Collection and Management Center, preservation number: ACCC 31573) and / or Penicillium polandicus strain No. 2 (preservation unit: China Center for Type Culture Collection, preservation number: No. M2010086), use PDB medium for liquid fermentation for 7 days, filter 2L of the fermentation broth with gauze, extract the obtained liquid with ethyl acetate, and perform rotary evaporation of the obtained organic phase to obtain crude extract I. The bacterial cells obtained were filtered with gauze, soaked in methanol, sonicated for 30 min, and then filtered. The obtained liquid was subjected to rotary evaporation to obtain crude extract II. The crude extracts obtained twice were mixed and prepared into an extract of 100 mg / mL with methanol. .
[0030] The Penicillium Poloniae extract is elut...
Embodiment 2
[0041] Embodiment 2, activity test
[0042] 1) Test the inhibitory activity of the fermented liquid extract of Penicillium polish 1 strain on the germination of Chinese cabbage and crabgrass
[0043] Experimental method: filter 2L of the 7-day fermentation broth with gauze, extract the obtained liquid with ethyl acetate, and perform rotary evaporation on the obtained supernatant to obtain crude extract I. The bacterial cells obtained were filtered with gauze, soaked in methanol, sonicated for 30 min, and then filtered. The obtained liquid was subjected to rotary evaporation to obtain crude extract II. The crude extracts obtained twice were mixed and prepared into an extract of 100 mg / mL with methanol. mother liquor.
[0044]Seed surface disinfection: the seeds (smooth surface, flat seeds, no cracking, complete appearance) are first rinsed with sterile water, then soaked with 70% ethanol for 2 minutes, then soaked with 2% sodium hypochlorite for 2 minutes, and finally with ste...
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