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Cladosporium ultratrichum and its use for promoting the synthesis of effective components in the root system of Danshen

An active ingredient, Cladosporium technology, applied in the field of endophytic fungi in the root system of Salvia miltiorrhiza Effect

Active Publication Date: 2021-01-26
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on endophytic bacteria in medicinal plants is still in its infancy, and so far there are few reports on the effects of endophytic fungal elicitors that can produce plant-derived bioactive compounds on the secondary metabolism of host plants. Bacteria and the use of plant endophytes for fermentation production, or inoculation of specific microorganisms in medicinal plants to obtain some important natural medicines or some authentic medicinal materials, will solve the problem of slow growth of certain medicinal plants, resource shortages, etc. The lack of drug sources and ecological damage caused by it have guiding significance

Method used

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  • Cladosporium ultratrichum and its use for promoting the synthesis of effective components in the root system of Danshen
  • Cladosporium ultratrichum and its use for promoting the synthesis of effective components in the root system of Danshen
  • Cladosporium ultratrichum and its use for promoting the synthesis of effective components in the root system of Danshen

Examples

Experimental program
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Effect test

Embodiment 1

[0050] The isolation culture method of embodiment 1, Cladosporium tenuissimum DF11, carries out following steps successively:

[0051] 1) Select a Salvia miltiorrhiza plant with a suitable size and grow healthily and plant it indoors, cut off the root of Salvia miltiorrhiza with a length of about 5 cm, and remove the soil attached to the root surface as much as possible.

[0052] 2) Rinse the intercepted root segment of Salvia miltiorrhiza with sterilized PBS solution for 2 times, and then rinse with sterile water for 2 times. Then soak the root section of Salvia miltiorrhiza in 200mL of sterilized PBS solution, and vibrate ultrasonically for 10min.

[0053] 3) Take out the root of Salvia miltiorrhiza, wash it with sterile water for 5 times, and mix the washing liquid with the PBS solution after ultrasonic vibration in the previous step to make a suspension.

[0054] 4) Take 500 μL of the suspension and evenly spread it on a PDA plate, and after incubating at 28° C. for 24 ho...

Embodiment 2

[0065] Embodiment 2, the cultivation of the aseptic seedling of Salvia miltiorrhiza, carry out the following steps successively:

[0066] 1) Select the full-grown seeds of Salvia miltiorrhiza, rinse with running water, treat with 75% alcohol for 1 minute, rinse with sterile water, treat with 5% NaClO solution for 1 to 2 minutes, rinse with sterile water for 2 to 3 times, and absorb it with sterile filter paper. Remove excess water, sow in MS medium to obtain aseptic seedlings of Salvia miltiorrhiza.

[0067] 2), proliferation seedling cultivation:

[0068] After the aseptic seedlings germinate, cut 1.5-2.0 cm stems with leaves and place them in MS medium in a tissue culture room at 25°C for cultivation until they grow into seedlings with 3-4 leaves, and then transplant them into the plant. In a 1300ml PVC plastic tissue culture bottle with 100ml of MS liquid medium and an appropriate amount of sterile substrate (a mixture of humus: perlite: vermiculite = 3:1:1 volume ratio), ...

Embodiment 3

[0069] Example 3: Effects of endophytic fungi of Salvia miltiorrhiza on the biosynthesis of active ingredients in Salvia miltiorrhiza:

[0070] The Salvia miltiorrhiza bacterin of the present invention and bacterial strain DF11 are carried out co-cultivation, carry out the following steps successively:

[0071] (a) Get the endophytic fungus DF11 strain of Salvia miltiorrhiza of the present invention, under aseptic conditions, pick a small amount of hyphae with an inoculation needle, insert in the sterilized potato dextrose liquid (PDA medium) fermentation bottle, in 28 ℃ activation culture for 6-7 days; dilute the culture solution to obtain a concentration of 10 6 Bacterial suspension with about CFU / ml.

[0072] (b) Inoculate 1 mL of bacterial suspension (concentration 10 6 About CFU / ml); the potting soil substrate for the co-cultivation of salvia miltiorrhiza and salvia miltiorrhiza is humus: perlite: vermiculite = 3:1:l (volume ratio) mixed cultivation substrate; cultivati...

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Abstract

The invention discloses a salvia miltiorrhiza endophytic fungus DF11, which is Cladosporium tenuissimum and has a preservation number of CGMCC No.16883. The invention also discloses the application of the above-mentioned salvia miltiorrhiza endophytic fungus DF11 at the same time: promoting the synthesis and growth of salvia miltiorrhiza root active ingredients; the active ingredients are tanshinone substances and salvianolic acid substances. The endophytic fungus of the salvia miltiorrhiza of the invention can effectively promote the root system development of the salvia miltiorrhiza through co-cultivation with the salvia miltiorrhiza plant, and can also effectively promote the biosynthesis of the effective components of the salvia miltiorrhiza, and has great application value.

Description

technical field [0001] The invention relates to an endophytic fungus of the root system of Danshen and its use for promoting the synthesis of effective components in the root system of Danshen. Background technique [0002] The traditional Chinese medicine Salvia miltiorrhiza (scientific name: Salvia miltiorrhiza Bge.) is the root of Salvia miltiorrhiza Bge. It has various effects such as promoting blood circulation and removing blood stasis, cooling blood and eliminating carbuncle, relieving pain, clearing away heart-fire and eliminating troubles. According to its traditional efficacy, Danshen is widely used in the treatment of cardiovascular and cerebrovascular diseases in modern times, and has achieved remarkable curative effect. The medicinal components of Danshen include water-soluble salvianolic acids and fat-soluble tanshinones. Water-soluble phenolic acid compounds, mainly salvianolic acid A, B, C, caffeic acid, danshensu, etc., have anti-thrombosis, anti-oxidation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G7/06A01G31/00C12R1/645
CPCA01G7/06A01G31/00C12N1/14C12N1/145C12R2001/645
Inventor 陈海敏梁宗锁马瑶张海花张晓丹杨东风胡秀芳
Owner ZHEJIANG SCI-TECH UNIV
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