Cladosporium cladosporioides strain with strong pathogenicity to diaphorina citri and application of Cladosporium cladosporioides strain

A technology of Cladosporium cladoides and citrus psyllids, which is applied in the fields of application, fungi, and methods of using spores, which can solve the problem of less available spores, and achieve the effect of strong pathogenicity and large spore production

Active Publication Date: 2021-05-07
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few effective use in production. Therefore, it is particularly important to develop more citrus psyllid biocontrol bacteria resources to effectively control citrus psyllid and citrus Huanglongbing

Method used

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  • Cladosporium cladosporioides strain with strong pathogenicity to diaphorina citri and application of Cladosporium cladosporioides strain
  • Cladosporium cladosporioides strain with strong pathogenicity to diaphorina citri and application of Cladosporium cladosporioides strain
  • Cladosporium cladosporioides strain with strong pathogenicity to diaphorina citri and application of Cladosporium cladosporioides strain

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Experimental program
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Effect test

Embodiment 1

[0027] Isolation and purification of embodiment 1 bacterial strain CLAD

[0028] Abnormally dead citrus psyllids were collected from citrus plants planted in the experimental base of the Institute of Fruit Trees, Guangdong Academy of Agricultural Sciences, Tianhe District, Guangzhou City, Guangdong Province. The collected citrus psyllid corpses were surface-sterilized with 75% alcohol solution, the sterilized samples were rinsed three times in sterilized water, placed on a PDA plate, and placed upside down in a 25°C incubator for cultivation. After colonies were formed, A single colony was picked for isolation and purification, and a pure strain was obtained after multiple transfer cultures, which was named strain CLAD, and stored in a 4°C refrigerator.

Embodiment 2

[0029] Identification and preservation of embodiment 2 bacterial strain CLAD

[0030] The total DNA of the strain CLAD was extracted by the Ezup kit, amplified by PCR using fungal universal primers, and sequenced. Using NCBI Blast for comparison analysis, it was found that the measured sequence (ITS1-5.8S rDNA-ITS2 sequence) and multiple strains of Cladosporium cladosporioides in GenBank (DCF1, daef25, GX36, FJAT-30992, etc.) The sequence similarity is as high as 99% to 100%. It was proved that the strain CLAD belonged to Cladosporium (Cladosporium), Cladosporium cladosporioides (Cladosporium cladosporioides), and it was named as Cladosporium cladosporioides (Cladosporium cladosporioides) CLAD. Wherein, the rDNA-ITS2 sequence size of the Cladosporium cladoides CLAD strain is 525bp, and its nucleotide sequence is shown in SEQ ID NO:1.

[0031] Subsequently, on November 17, 2020, the Cladosporium cladoides CLAD strain was registered and preserved at the Guangdong Microbial Cul...

Embodiment 3

[0037] Morphological characteristics of embodiment 3 Cladosporium CLAD bacterial strain

[0038] Cladosporium cladoides CLAD strain grows well on potato dextrose medium (PDA), as figure 1 As shown, at 27°C for 10 days, the diameter of the colony can reach 6.5 cm, the colony is round, and the colony is dark green and velvet at first. The front of the colony cultivated for 10 days was dark green cotton flocculent, the middle part was light yellow and raised, and the back of the colony was dark green. Hyphae branched with septa and branches see appendix figure 2 . The conidia are oval, with a size of 14.7-20.0μm×23.3-63.3μm, such as image 3 shown.

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Abstract

The invention belongs to the technical field of biological control, and particularly relates to a Cladosporium cladosporioides strain with strong pathogenicity to diaphorina citri and application of the Cladosporium cladosporioides strain. A CLAD strain is separated from an abnormal death diaphorina citri body of a citrus plant, the strain is identified as Cladosporium cladosporioides, named as Cladosporium cladosporioides CLAD, and preserved in Guangdong Microbial Culture Collection Center on November 17, 2020, and the preservation number is GDMCC No:61248. The strain has a large spore production amount, spore liquid has strong pathogenicity to nymphs and adults of diaphorina citri at normal temperature and normal humidity, and is expected to be developed as biocontrol bacteria of diaphorina citri, and more choices are provided for green biological control of diaphorina citri.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a cladosporium strain with strong pathogenicity to citrus psyllids and an application thereof. Background technique [0002] Citrus wood wind (Diaphorinacitri Kuwayama) belongs to Homoptera (Homoptera), wood wind family (Chermidae), is the main pest of Rutaceae plants such as citrus, lemon, wampum, mulberry, wolfberry, etc., and also spreads citrus greening disease. the only natural insect vector. Citrus huanglongbing is the primary problem in citrus planting industry, which has caused serious economic losses to fruit farmers and greatly hindered the development of citrus industry in my country and the world. [0003] At present, chemical control measures are mostly used for the control of citrus psyllids. Although the purpose of killing pests is achieved through the extensive use of chemical pesticides, the frequent use of chemical pesticides has caused p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/30A01P7/04C12R1/645
CPCA01N63/30C12N3/00
Inventor 张瑞敏曾继吾吴文黄永敬朱从一
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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