Application of isoliquiritin

A technology of isoglycyrrhizin and licorice, applied in the field of plant fungicides, can solve problems such as not seen, and achieve the effect of solving the problem of resistance

Inactive Publication Date: 2009-09-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the specific study of the antibacterial activity of the active ingredients in the licorice extract to plant pathogenic bacteria will imp

Method used

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  • Application of isoliquiritin
  • Application of isoliquiritin
  • Application of isoliquiritin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of embodiment 1 isoliquiritin (isoliquiritin)

[0020] The roots of licorice were collected and dried in a 50°C electric constant temperature blast drying oven; pulverized by a pulverizer and passed through a 40-mesh sieve. The obtained plant powder is sealed in a sealed bag and stored in a dark place for extraction.

[0021] The present invention adopts the existing conventional ultrasonic extraction method to prepare the licorice extract, and can also refer to other existing conventional techniques for extraction and preparation. Isoliquiritin is obtained by separating, purifying and identifying the structure of the prepared methanol extract.

Embodiment 2

[0022] Embodiment 2: The inhibitory effect of isoliquiritin (isoliquiritin) on peronophythora (Peronophythora Chen) pathogens

[0023] The extract was dissolved with 75% methanol as a solvent, and then mixed with PDA medium to make a poisonous medium, and the inhibitory effect of different concentrations of extracts on the mycelial growth of Ponosophthora litchi was determined by the method of mycelial growth rate. The specific operation is:

[0024] Mycelia growth rate method: Use 75% methanol as a solvent to prepare isoliquiritin (isoliquiritin) into a certain concentration of the test drug solution, accurately draw 1mL of the drug solution and add it to 49mL of melted PDA medium (about 50°C) and mix Pour it evenly into sterilized petri dishes (diameter 9cm), pour each bottle evenly into 3 petri dishes, and prepare the poisonous culture medium with the required concentration. The control was mixed with the same volume of sterile water or 75% methanol. The test pathogen Pyt...

Embodiment 3

[0030] Experiments have shown that the inhibition rate of isoliquiritin to the mycelial growth of Pythora lychee downy mildew was significantly higher than that of the treatment, and the inhibition rate reached 89.443%, 80.683%, 80.683%, 63.830%, showing a strong antibacterial effect. Embodiment 3: the inhibitory action of isoliquiritin (isoliquiritin) to downy mildew (Peronophythora Chen) pathogen

[0031] Adopt the glass slide method (Fang Zhongda, 1998) to measure: the sporangia that will be cultivated on the PDA substratum for 10 days with aseptic water to wash the sporangia of Peronospora litchie, add medicinal liquid to be prepared into spores containing various concentrations of medicinal liquid For the suspension of sporangia, adjust the concentration of sporangia to about 40-60 sporangia per field of view (10×10 times), take 50 μL of the sporangia suspension and drop it on the slide, and then place the slide on a place where a piece of filter paper has been placed. I...

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Abstract

The invention discloses an application of isoliquiritin, particularly the application of isoliquiritin to plant disease prevention. Through indoor biological measurement and pot experiment, the invention provides a better preventing and treating application of isoliquiritin which is active ingredient in liquorice methyl alcohol extract to common plant disease such as Peronophythora Chen, Phytophthora capsici Leonian, Cladosporium LK. ex Fr, Sclerotinia Colletotrichum Corda and the like. The invention provides a new design for liquorice and extract thereof applied to plant disease prevention, provides a new development resource for plant substitution chemical synthesis bactericide, and has important significance for solving the problem that pathogenicbacteria has increasingly remarkable resistance to drug.

Description

technical field [0001] The invention belongs to the technical field of plant fungicides, and in particular relates to a new application of isoliquiritin, an active ingredient in licorice extract, in preventing and treating plant diseases. Background technique [0002] In recent years, with the continuous development of phytopathology, biology and other related disciplines, the research on fungicides in pesticides has entered a new period. The emergence of high-efficiency systemic fungicides such as Strobilurn fungicides, on the one hand, has made a breakthrough in the prevention and control of plant diseases, and on the other hand, it has also caused the problem of pathogenic bacteria's resistance to the fungicides to become increasingly prominent. Seeking pollution-free pesticides with good environmental compatibility and long-lasting effects on pathogenic bacteria is particularly important today when green food is increasingly valued. [0003] Natural products often exhib...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P3/00
Inventor 罗建军胡美英钟国华郝卫宁曾勇林庆胜
Owner SOUTH CHINA AGRI UNIV
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