Primers for identifying pathogenic microorgansims of apple tree branch and trunk rot and disease degree grading method

A grading method, apple tree technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as neglect and inability to separate and purify

Active Publication Date: 2021-06-18
TIANSHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there are many unculturable microorganisms, or microorganisms whose growth is limited under the existing culture conditions, may play a role in the process of apple tree branch rot, but they have been ignored because they cannot be isolated and purified

Method used

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  • Primers for identifying pathogenic microorgansims of apple tree branch and trunk rot and disease degree grading method
  • Primers for identifying pathogenic microorgansims of apple tree branch and trunk rot and disease degree grading method
  • Primers for identifying pathogenic microorgansims of apple tree branch and trunk rot and disease degree grading method

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Embodiment Construction

[0081] The pathogenic bacteria identification primer of apple tree branch rot of the present invention, the nucleotide sequence of identification primer is:

[0082] Methylobacterium brachiatum-F: 5'-AGGGAACCCCCGGAYCTCTC-3',

[0083] Methylobacterium brachiatum-R: 5'-TCCACGCCGTAAACGAYGAA-3';

[0084] Sphingomonas aquatilis-F: 5'-GTATCTAATCCTGTTTGCTYCC-3',

[0085] Sphingomonas aquatilis-R: 5'-GCTCAACTCCAGAAYTGCC-3';

[0086] Aureobasidium pullulans-F: 5'-AAATTCTACTACGCYTAAAGCC-3',

[0087] Aureobasidium pullulans-R: 5'-CATCGAATCTTTGAAYGCAC-3';

[0088] Valsa mali-F: 5'-GAAATGACGCTCGAAYAGGC-3',

[0089] Valsa mali-R: 5'-TGCACCCAGAAAYCCTTTGT-3';

[0090] Botryococcus braunii-F: 5'-CTACCTTGTTACGACYTCACC-3',

[0091] Botryococcus braunii-R: 5'-GAGCCAACCTCAAAAAYCC-3';

[0092] Cladosporium allicinum-F: 5'-CACCCTTTAGCGAATAGYTTCC-3',

[0093] Cladosporium allicinum-R: 5'-GTCATTTCACCACTYAAGCC-3';

[0094] Didymella keratinophila-F: 5'-GAGACAAACACCYAAC...

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Abstract

The invention relates to the technical field of identification of pathogenic microorganisms, in particular toprimers for identifying pathogenic microorganisms of apple tree branch and trunk rot and a disease degree grading method. The identification primers have good specificity, can conduct specific amplification on genomic DNA of three strains of methylobacterium brachiatum, sphingomonas aquatilis and aureobasidium pullulans in robust fruit trees, two strains of valsa mali and botryococcus brauni in diseased fruit trees, and four strains of sagonosporopsis lupini, cladosporium allicum, didymella keratinophil and cladosporium delicatulum in withered fruit tree after disease, are free of primer dimers and non-specific amplification, can be used for detecting a diseased state and a diseased degree of apple tree branch rot in an incubation period with a good effect, further judges the diseased state and the diseased degree of the apple tree branch rot, helps establish a detection and grading standard of the diseased degree, and provides a technical support for early detection and scientific prevention and treatment of the apple tree branch rot.

Description

technical field [0001] The invention relates to the technical field of identification of pathogenic bacteria, in particular to primers for identification of pathogenic bacteria of apple tree branch rot and a disease degree grading method. Background technique [0002] The branch rot of apple trees is called "cancer" of fruit trees by fruit farmers. The diseased fruit trees will not only damage the branches or die the whole plant, but even spread to the whole orchard and surrounding orchards. It has become the main factor restricting the development of the apple industry. one. [0003] In the prior art, the identification of pathogenic bacteria is mainly based on the isolation and culture of microorganisms from diseased tissues combined with DNA barcode sequence analysis. The isolation and culture of pathogenic bacteria mainly uses PDA medium, and the DNA barcode sequences include rDNA-ITS, ribosomal large subunit fragments, transcription elongation factor and β-tubulin, etc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/689C12Q1/6895C12Q1/686C12Q2561/113C12Q2563/107C12Q2545/114Y02A50/30
Inventor 马伟超李一婧谢春洋杨江生
Owner TIANSHUI NORMAL UNIV
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