Application of perylenequinone compound in preparation of feed for preventing and controlling ruminant viruses

A technology for ruminants and compounds, applied in animal feed, animal feed, applications, etc., can solve the problems that hypericin cannot be effectively used, easily decomposed, and the chemical properties of hypericin are unstable

Inactive Publication Date: 2011-06-29
JIANGNAN UNIV
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical properties of hypericin are unstable, and it is easy to decompose when exposed to light. In practical applications, a considerable part of hypericin cannot be effectively utilized.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of perylenequinone compound in preparation of feed for preventing and controlling ruminant viruses
  • Application of perylenequinone compound in preparation of feed for preventing and controlling ruminant viruses
  • Application of perylenequinone compound in preparation of feed for preventing and controlling ruminant viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: In vitro antiviral experiment of perylenequinone compounds

[0047] (1) Preparation of cells for in vitro antiviral experiments

[0048] BHK-21 cells (Baby hamster kidney cells) were used to study the effect of perylenequinone compounds on foot-and-mouth disease virus (FMDV). Growth medium: buy MEM (modified eagle medium) medium, add NaHCO 3 2.0g / L, 2% fetal bovine serum, 1% penicillin, 1% streptomycin. After the BHK-21 cells were digested with 0.25% trypsin, the cells were dispersed by pipetting, and diluted with MEM growth medium to 1×10 6 Cells / mL, add to 96-well cell culture plate, 37°C, 5% CO 2 Cultured in an incubator, after the cells grew into a single layer of cells, the in vitro antiviral test was carried out.

[0049] The effect of perylenequinone compounds on bluetongue virus (BTV) was studied with Vero cells (African green monkey kidney cells, Verda Reno). Growth medium: add 110mg / L sodium pyruvate, 300mg / L L-glutamine, 200mg / L NaHCO to the p...

Embodiment 2

[0078] Embodiment 2: Anti-FMDV experiment of sheep added to feed by solid-state fermented hypocrellin

[0079] The basic diet formula of the sheep in the experiment was:

[0080] Concentrated feed formula: 46% corn, 20% bran, 30% cotton meal or rapeseed meal, 1% stone powder, 1% calcium hydrogen phosphate, 1% salt, 1% premix (which can provide vitamin A 1200IU per kg feed , Vitamin D 32500IU, Vitamin E 30IU, Vitamin K 3mg, Riboflavin 4mg, Niacin 40mg, Pantothenic Acid 15mg, Choline Chloride 400mg. Folic Acid 700g, Thiamine VB 1.5mg, Vitamin B 3mg, Biotin 100g, Zn 80mg, Mn 20mg, Fe 83mg, Cu 25mg), particle size 1-2mm. In the final diet of sheep: grass hay accounts for 45%, silage corn 23%, and concentrate 32%.

[0081] Hypocretin is produced by solid-state fermentation, corn is the main raw material, and 2% glucose and 0.2% KH based on the weight of corn are added 2 PO 4 , 0.04% MgSO 4 ·7H 2 O. Add water and mix the material to make it wet, control the ratio of material ...

Embodiment 3

[0093] Example 3: Anti-bovine BVDV virus experiment of liquid fermented hypocretin added to feed

[0094] Experimental basic diet: corn 48%, bran 14%, cottonseed cake 35%, stone powder 1.0%, bone meal 0.5%, salt 1.0%, sodium bicarbonate 0.5%.

[0095]Hypocretin is produced by liquid fermentation method, and the culture medium per liter consists of: glucose 20g, peptone 50g, potato 200g, (NH 4 ) 2 HPO 4 2g, KH 2 PO 4 2g, MgSO 4 0.5g / L, sterilized at 121°C for 20min, then add 10% liquid bamboo yellow fungus seeds after cooling. Both the initial pH and the pH of the fermentation process are natural, the fermentation temperature is 30° C., and the fermentation ends in 72 hours. The fermented liquid was removed by centrifugation to obtain mycelium, which was dried at 60°C. It was determined that hypocrellin A accounted for 8.1%, hypocrellin B accounted for 1.3%, and the total amount of other perylenequinone compounds accounted for 0.2%.

[0096] Forty calves with an average ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses application of a perylenequinone compound in preparation of a feed for preventing and controlling ruminant viruses, and belongs to the technical field of bioengineering and agriculture. The perylenequinone compound is added into a ruminant feed to prepare the feed for preventing and controlling the ruminant viruses such as foot-and-mouth disease virus, cell pathogenetic bovine viral diarrhea virus-mucosal disease virus, bluetongue virus, capripoxvirus, epuine morbillivirus and the like. The perylenequinone compound comprises hypocrellin, elsinochrome, phleichrome, cladosporium, calphostin, cercosporin and the like; and the perylenequinone compound has a series of advantages of extracting convenience, good stability, no obvious side effect in oral administration, quick metabolism and the like. The dosage of the prepared feed for preventing the ruminant viruses is 1 to 10mumol perylenequinone compound per kg weight every day, the dosage of the prepared feed for treating the ruminant viruses is 10 to 100mumol perylenequinone compound per kg weight, the effect of preventing and treating the viruses is very remarkable, meanwhile, the perylenequinone compound can be obtained by modern biotechnology, and good economic and social benefits can be generated.

Description

technical field [0001] Application of a perylenequinone compound in preparing feed for preventing and controlling ruminant viruses such as cattle, sheep, donkeys and horses. The invention relates to adding a perylenequinone compound to feed for preparing feed for preventing and controlling ruminant viruses. It belongs to the field of biological engineering and agricultural technology. Background technique [0002] In recent years, with the development of my country's economy and the improvement of living standards, the milk and beef industries have become the highlights of the restructuring of animal husbandry. At the same time, the sheep industry is also a pillar industry for the economic development of my country's frontier and minority areas. [0003] However, with the continuous expansion of the breeding scale, large-scale epidemic outbreaks will also be brought, causing huge losses to the farms. Viral diseases, in particular, are characterized by strong infectivity an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/17A23K1/18A23K20/111A23K20/195A23K50/10
Inventor 蔡宇杰廖祥儒朱建航丁彦蕊张大兵
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap