Dura graft, preparation method and applications in dural damage repair thereof

A dura mater, allogeneic technology, applied in the field of tissue engineering of biomedical materials, can solve the problems of less application, more residual antigen components, and no consideration of patch strength, elasticity, elongation, etc.

Active Publication Date: 2017-04-26
BEIJING JAYYALIFE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former removes cells and antigenic components in skin tissue by chemical detergents combined with other auxiliary reagents, such as removing cells in a solution containing EDTA and trypsin or DispaseII enzyme, and then incubating them in TritonX-100; the latter is prepared by Hypertonic sodium chloride and SDS preparation, but more antigenic components will remain
In addition, there are also preparation methods such as trypsin digestion method, repeated freezing and thawing method, NaOH erosion method, and balanced salt i...

Method used

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  • Dura graft, preparation method and applications in dural damage repair thereof
  • Dura graft, preparation method and applications in dural damage repair thereof
  • Dura graft, preparation method and applications in dural damage repair thereof

Examples

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Effect test

Embodiment 1

[0056] Example 1 Preparation method of acellular allogeneic dermal matrix

[0057] The present invention provides a method for preparing an acellular allogeneic dermal matrix, and a preferred preparation embodiment includes performing the following steps in order:

[0058] Put the raw materials into a container containing a cross-linking agent solution and soak for 4 hours. The cross-linking agent is genipin and N-hydroxysuccinimide, and the cross-linking agent is genipin and N-hydroxysuccinimide The mass ratio of the cross-linking agent to the allograft skin is 1.2:1, soaked for 8 hours. Take out the raw material, put it into another container filled with physiological saline solution, soak for 56 hours at 5°C, change the physiological saline solution every 8 hours, and obtain the semi-finished product A.

[0059] Put the semi-finished product A into 0.2% trypsin-dispase enzyme solution (w / v) for soaking and shaking, wherein the mass ratio of trypsin to dispase enzyme is 2:1...

Embodiment 2

[0068] Example 2 Preparation method of acellular allogeneic dermal matrix

[0069] The present invention provides a method for preparing an acellular allogeneic dermal matrix, and a preferred preparation embodiment includes performing the following steps in order:

[0070] Raw material is put into the container that is filled with cross-linking agent solution, soaks 4 hours, and cross-linking agent is genipin and N-hydroxysuccinimide, and the mass ratio of genipin and N-hydroxysuccinimide is 4:1, the mass ratio of cross-linking agent to skin allograft is 1.4:1, soak for 8 hours. Take out the raw material, put it into another container filled with physiological saline solution, soak for 64 hours at 5°C, change the physiological saline solution every 8 hours, and obtain the semi-finished product A.

[0071] Put the semi-finished product A into 0.3% trypsin-papain solution (w / v), soak and shake, wherein, the mass ratio of trypsin and papain is 2.5:1, adjust the pH value to 7.2 w...

Embodiment 3

[0080] Example 3 Preparation method of acellular allogeneic dermal matrix

[0081] The present invention provides a method for preparing an acellular allogeneic dermal matrix, and a preferred preparation embodiment includes performing the following steps in order:

[0082] Raw material is put into the container that is filled with cross-linking agent solution, soaks 5 hours, and cross-linking agent is genipin and N-hydroxysuccinimide, and the mass ratio of genipin and N-hydroxysuccinimide is 3:1, the mass ratio of cross-linking agent to allograft leather is 1.5:1, soak for 8 hours. Take out the raw material, put it into another container filled with physiological saline solution, soak for 56 hours at 5°C, change the physiological saline solution every 8 hours, and obtain the semi-finished product A.

[0083] Put the semi-finished product A into 0.2% trypsin and bromelain solution (w / v) for soaking and shaking, wherein the mass ratio of trypsin and bromelain is 1:1, adjust the...

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Abstract

The invention provides a dura graft and a preparation method thereof. The dura graft is prepared from an acellular dermal matrix. The acellular dermal matrix is obtained from allogenic skin by crosslinking treatment, digestion treatment with a protease solution, decellularization with a hypertonic saline solution containing a surfactant, DNA degradation, amino acid nutrition treatment and freeze drying. The invention also provides applications of the dura graft in preparing repair materials used in the treatment of dural damage. The acellular dermal matrix is obtained from human skin tissue and undergoes physicochemical and biochemical treatment, so that all the components that may cause immunological rejection are removed. A fibrous stereoscopic scaffold structure of the original tissue is completely reserved, and new vessels and fibroblasts rapidly grow in the dura graft after implantation. Therefore, the dura graft has good clinical effects.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering of biomedical materials, and in particular relates to a dura mater patch, a preparation method and an application in repairing dura mater damage. Background technique [0002] The human dura mater is a thick and tough double-layer membranous tissue on the surface of the brain tissue, which is close to the inside of the skull and is an important barrier to protect the brain and prevent the communication between the cerebrospinal fluid and the outside world. Open craniocerebral injury (industry, traffic, war, etc.), tumor erosion, inflammatory destruction, and congenital diseases can cause dural defects, resulting in complications such as cerebrospinal fluid overflow and intracranial infection. When the brain loses its dura mater coverage, it will inevitably adhere to the surrounding tissue, and the surrounding new blood vessels will grow into the brain tissue to form a scar. This is the ...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/36
Inventor 孙继煌石清东吴文华
Owner BEIJING JAYYALIFE BIOTECH CO LTD
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