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Vascularization support for tissue engineering and construction method of vascularization support

A tissue engineering scaffold, tissue engineering technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of hindering cell migration, long angiogenesis time, blood flow disorder, etc., to shorten the vascularization time and shorten the vascularization. time, the effect of reducing the time to vascularization

Inactive Publication Date: 2016-11-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Main reasons: 1. Angiogenesis takes a long time, the degradation of polymer scaffold materials takes time, and the space it has can only meet the proliferation and migration of endothelial cells, and the scaffold material itself will hinder cell migration in the later stage, while the acellular matrix has a dense structure, the number of cells grown in per unit time is small; 2. The existing scaffold materials can only realize the unidirectional growth of seed cells, the new blood vessels are disordered, and the blood flow is chaotic, which limits the flow of fresh blood into the new tissue
[0007] In short, the existing tissue engineering vascularized scaffold materials are still unable to simulate the microenvironment required for angiogenesis in organisms to the greatest extent, and obtain a mature vascular network

Method used

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  • Vascularization support for tissue engineering and construction method of vascularization support
  • Vascularization support for tissue engineering and construction method of vascularization support

Examples

Experimental program
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Effect test

Embodiment 1

[0048] The tissue engineering vascularized stent of this embodiment is prepared by the following method:

[0049] (1) Preparation of porcine decellularized pericardium grafted with VEGF

[0050] Refer to patent CN101274106 B (a method for preparing acellular matrix) to prepare porcine decellularized pericardium, the specific steps are as follows: pretreat porcine pericardium tissue, and then add the pretreated porcine pericardium tissue to a mixture containing phospholipids The porcine decellularized pericardium is prepared by soaking in the enzyme solution through hypotonic or isotonic immersion, and then washed to obtain the porcine decellularized pericardium. Wherein, the concentration of phospholipase is less than 10000U / mL, the temperature range of the solution is 0-56°C, and the pH value range is 5-12.

[0051] Immerse 1 g of dry weight decellularized pericardium in 5 mL of 0.05 mol / L MES (2-(N-morpholine) ethanesulfonic acid monohydrate) buffer (pH=5.60) for 0.5 h; EDC...

Embodiment 2

[0072] The tissue engineering vascularized stent of this embodiment is prepared by the following method:

[0073] (1) Preparation of dog decellularized bladder loaded with strontium ions

[0074] Refer to patent CN 101274106 B (a method for preparing acellular matrix) to prepare dog decellularized bladder, then immerse 2g of dry weight dog decellularized bladder into 50mL of 0.05mol / L strontium chloride in PBS (pH=7.2) solution, 4°C Incubate overnight, rinse with PBS 4 times at room temperature, each time for 10 min, and obtain strontium ion-loaded dog decellularized bladder.

[0075] (2) Pretreatment of dog decellularized bladder loaded with strontium ions

[0076] Take the dog decellularized bladder loaded with strontium ions prepared in step (1) and immerse it in 5 mL of normal saline, let it stand overnight in a refrigerator at 4 °C, cut it into a membrane with a diameter of 1 mm, rinse it with deionized water three times, and store it at -20 ℃ refrigerator for 4 h, and ...

Embodiment 3

[0089] The tissue engineering vascularized stent of this embodiment is prepared by the following method:

[0090] (1) Pretreatment of acellular matrix

[0091] Refer to patent CN 101274106 B (a method for preparing acellular matrix) to prepare acellular amnion, the specific steps are as in Example 1, the only difference is that the porcine pericardium tissue in Example 1 is replaced with amnion tissue;

[0092] Take a dry weight of 0.4g decellularized amniotic membrane and immerse it in 5mL of normal saline. After 4 hours, use an ophthalmic trephine to cut it into a membrane piece with a diameter of 1cm. Let it stand overnight in a refrigerator at 4°C. ℃ refrigerator for 4 hours, and then implemented freeze-drying.

[0093] (2) Preparation of decellularized amniotic membrane / PLGA composite scaffold

[0094] Use a 500 μm diameter acupuncture needle to place the cell amniotic membrane in step (1) in accordance with figure 1 The model shown (diameter 1.5 cm) is arranged (5 x 8...

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Abstract

The invention belongs to the field of tissue engineering, and discloses a vascularization support for tissue engineering and a construction method of the vascularization support. The construction method includes (1), pre-treating an acellular matrix to remove impurities possibly introduced in decellularization and storage processes; (2), producing an acellular matrix / composite material compound support according to a tissue engineering support forming method; (3), post-treating the acellular matrix / composite material compound support obtained in the step (2), and removing residual substances in the production process to obtain the vascularization support for tissue engineering. The construction method has the advantages that the method is based on basic principles in organism vascularization processes and is conformity to migration and multiplication features of endothelial cells, microenvironments for organism vascularization are simulated to the greatest extent, the cellular matrix / composite material compound support is construed to develop advantages of both the acellular matrix and a composite material, and the support product capable of meeting tissue engineering vascularization requirements is constructed.

Description

technical field [0001] The invention belongs to the field of tissue engineering, in particular to a tissue engineering vascularized stent and a construction method thereof. Background technique [0002] In organisms, almost all tissues exchange nutrients, oxygen, and metabolites through the fine branch network of the blood circulatory system. After the tissue engineering scaffold is implanted into the organism, the cells adhering to the scaffold material will lose nutrients and oxygen supply and will die in large numbers, resulting in the survival rate of the seed cells and affecting the application range of tissue engineering products. Tissue engineering vascularization is to aim at the limitation of non-nutrient metabolism of tissue engineering products after implantation, and carry out procedural vascular construction before implantation, in order to solve the problem of nutrient metabolism of tissue engineering products after implantation. [0003] As a hot spot in tiss...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/18A61L27/56
CPCA61L27/3604A61L27/18A61L27/3679A61L27/56A61L2430/22C08L67/04C08L77/04
Inventor 屠美赵巨鹏武征
Owner JINAN UNIVERSITY
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