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Compound tissue engineering scaffold containing PLGA (poly(lactic-co-glycolic acid) strengthening net, and preparation method and application thereof

A tissue engineering scaffold and reinforcing mesh technology, applied in medical science, prosthesis, surgery, etc., to achieve the effect of facilitating adhesion

Inactive Publication Date: 2011-09-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the problem of mechanical properties of existing tissue engineering scaffolds, the first object of the present invention is to provide a biocompatibility, excellent mechanical properties, and a specific three-dimensional porous structure, which can perfectly meet the needs of tissue engineering tissues and organs. Composite tissue engineering scaffolds containing PLGA reinforced mesh to promote the filling and healing of tissue defects and improve the quality of healing

Method used

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  • Compound tissue engineering scaffold containing PLGA (poly(lactic-co-glycolic acid) strengthening net, and preparation method and application thereof
  • Compound tissue engineering scaffold containing PLGA (poly(lactic-co-glycolic acid) strengthening net, and preparation method and application thereof
  • Compound tissue engineering scaffold containing PLGA (poly(lactic-co-glycolic acid) strengthening net, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The preparation of embodiment 1PLGA reinforced net

[0059] Polylactic acid (LA) and polyglycolic acid (GA) monomers are mixed at a molar ratio of 1:1. After being fully mixed, the bulk is mixed at a high temperature. After being melt-spun into a thread, the PLGA reinforced mesh is woven. The PLGA reinforced mesh has a thickness of 200-3000 μm. The diameter of a single PLGA braided wire is between 10 and 200 μm; the mesh is cleaned with acetone, ultrasonically cleaned with pure water, and vacuum heat-set to obtain a PLGA reinforced mesh.

[0060] The above-mentioned polylactic acid (LA) and polyglycolic acid (GA) monomers can also choose any component ratio of 1:1 to 1:99 by mole.

Embodiment 2

[0061] Example 2 Ultrastructural Observation of the Composite Tissue Engineering Scaffold Containing PLGA Reinforcing Net under Scanning Electron Microscope

[0062] Collagen and chitosan were made into 0.5% acetic acid solution with a mass fraction of 0.5%, then the collagen solution and chitosan solution were mixed according to the volume ratio of 9:1, fully stirred evenly, and set aside; the PLGA reinforced net was cut into 4.5cm ×4.5cm in size, spread it flat on the flat plate, and then press a “frame” mold with a thickness of 1cm and a size of 4cm×4cm tightly on the net, and then inject 3.2mL of collagen / chitosan solution to make it evenly Infiltration of PLGA reinforced mesh; place PLGA reinforced mesh, collagen / chitosan solution together with the mold at 4°C for 24 hours, freeze at -20°C for 24 hours, and then freeze-dry in a freeze dryer for 16 hours; freeze-dry The composite scaffold was cross-linked under vacuum dry heat at 105 °C for 24 hours, and then cross-linked ...

Embodiment 3

[0064] Example 3 In vivo implantation experiment of composite tissue engineering scaffold containing PLGA reinforcement mesh

[0065] Collagen and chitosan were made into 0.5% acetic acid solution with a mass fraction of 0.5%, then the collagen solution and chitosan solution were mixed according to the volume ratio of 9:1, fully stirred evenly, and set aside; the PLGA reinforced net was cut into 4.5cm ×4.5cm in size, spread it flat on the flat plate, and then press a “frame” mold with a thickness of 1cm and a size of 4cm×4cm tightly on the net, and then inject 3.2mL of collagen / chitosan solution to make it evenly Infiltration of PLGA reinforced mesh; place PLGA reinforced mesh, collagen / chitosan solution together with the mold at 4°C for 24 hours, freeze at -20°C for 24 hours, and then freeze-dry in a freeze dryer for 16 hours; lyophilize The composite scaffold was cross-linked under vacuum dry heat at 105 °C for 24 hours, and then cross-linked in a mixed solution of EDAC (40 ...

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Abstract

The invention relates to a scaffold material for tissue engineering construction and a preparation method thereof, and particularly relates to a compound tissue engineering scaffold containing a PLGA (poly(lactic-co-glycolic acid) strengthening net, and a preparation method and application thereof. The compound tissue engineering scaffold containing a PLGA strengthening net comprises a porous scaffold having good biocompatibility and a PLGA strengthening net, wherein the PLGA strengthening net is formed by weaving PLGA fibers, and the PLGA strengthening net is tightly combined with the porous scaffold. The compound tissue engineering scaffold containing a PLGA strengthening net has mechanical property similar to that of the acellular dermal matrix, and is more beneficial to the maintenance of three-dimensional porous structures of the scaffold, thereby being more beneficial to the ingrowth of cells, blood vessels and tissues; and because of the good biocompatibility of collagen, chitosan and other naturally-derived macromolecules, the biological performance of the compound scaffold is maintained, thereby being beneficial to the adhesion, propagation and secretion of cells. Thus, the compound tissue engineering scaffold is a good renewable material applicable to the construction of tissues and organs of tissue engineering.

Description

technical field [0001] The invention relates to a scaffold material for tissue engineering construction and a preparation method thereof, in particular to a composite tissue engineering scaffold containing a PLGA reinforcing net, a preparation method and application thereof. Background technique [0002] Tissue defect or organ failure caused by various injury factors is the enemy of human health. Taking the dermis as an example, the dermis is located between the epidermis and the subcutaneous tissue, and plays an important role in maintaining the elasticity and toughness of normal skin. Once the dermal tissue is severely damaged, it cannot regenerate. Clinically, a large number of scar formation and scar contracture in the later stage of simple autologous cut-thick skin transplantation and simple epidermal cell membrane transplantation also prove the important role of dermal tissue from another perspective. [0003] At present, there are three main ways to deal with tissue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/20A61L27/18A61L27/56A61L31/06A61L31/04A61L31/14
Inventor 韩春茂王新刚于玮洁胡信雷孙华凤高长有胡行有传刚
Owner ZHEJIANG UNIV
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