Method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid through ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQD) coupling technique

A technology of vancomycin and tobramycin, which is applied in the field of medicine, achieves clinical application prospects and good effects, good clinical application prospects, and the effect of reducing toxic and side effects

Active Publication Date: 2012-07-04
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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There is no literature report or patent publication on the method of simultan...

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  • Method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid through ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQD) coupling technique
  • Method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid through ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQD) coupling technique
  • Method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid through ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQD) coupling technique

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Embodiment Construction

[0051] A detailed description will be given below of specific embodiments of the present invention.

[0052] The invention relates to a method for simultaneously determining the contents of vancomycin and tobramycin in tissue drainage fluid by ultra-high liquid chromatography-triple quadrupole mass spectrometry, and the method includes the following steps.

[0053] 1) Preparation of reference substance

[0054] Accurately weigh 100 mg of vancomycin reference substance and 10 mg of tobramycin reference substance, place them in 10 mL volumetric flasks, add pure water to dissolve, and quantitatively prepare vancomycin reference substance solutions with a mass concentration of 10 mg / mL and 1 mg of tobramycin reference substance. / mL of tobramycin reference substance solution, set aside.

[0055] 2) Preparation of internal standard solution

[0056] Accurately weigh 5.0 mg of atenolol reference substance, place it in a 10 mL volumetric flask, add pure water to dissolve, accuratel...

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Abstract

The invention relates to the field of drugs, in particular to a detection method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid. A method for simultaneously determining content of vancomycin and tobramycin in tissue drainage liquid through an ultrahigh performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQD) coupling techniquecomprises the following steps of: 1) preparing reference samples; 2) preparing internal standard solution; 3) preparing sample solution; 4) preparing mobile-phase solution; 5) setting chromatographicconditions; 6) optimizing mass spectrometric conditions; 7) determining the samples; 8) preparing gradient-concentration reference sample solution; 9) preparing a standard curve; and 10) analyzing and computing data. The method has the characteristics that: 1) the method is advanced; 2) the method is rapid and high-efficiency the loss is low; and 3) the clinical application prospect and the effect are good.

Description

technical field [0001] The invention relates to a medicine field, in particular to a detection method for simultaneously measuring the contents of vancomycin and tobramycin in tissue drainage fluid. Background technique [0002] Vancomycin is a complex glycopeptide antibiotic produced by Streptomyces, with the molecular formula C 66 h 74 C l N 9 o 24 . It is mainly used for systemic and intestinal infections caused by Staphylococcus (including penicillin-resistant and neopenicillin-resistant strains), Clostridium difficile, etc., such as endocarditis, sepsis, and pseudomembranous enteritis. Vancomycin can inhibit the synthesis of bacterial cell walls, and has a strong effect on Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, etc., and has a good effect on Bacillus difficile, Bacillus anthracis, and Bacillus diphtheria. No cross-resistance with other antibiotics, very few drug-resistant strains. [0003] Tobramycin is an aminoglycoside antibiot...

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Application Information

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IPC IPC(8): G01N30/02
Inventor 董宇寿旦吴人杰沈立锋张春
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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