Cornea metaphase preservation liquid and preparation method thereof

A technology of preservation solution and cornea, which is applied in the preservation, application, and animal husbandry of human or animal bodies. It can solve the problems of impossibility of penetrating corneal transplantation and reduction, and maintain the activity and normal shape of corneal endothelial cells, enhance Effects on viability and less cell fusion

Active Publication Date: 2016-02-03
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many studies have shown that the number of viable corneal endothelial cells will gradually decrease with the prolongation of storage time, so that they cannot be used for...

Method used

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  • Cornea metaphase preservation liquid and preparation method thereof
  • Cornea metaphase preservation liquid and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A medium-term corneal preservation solution, which is a MEM cell culture medium containing chondroitin sulfate, low-molecular dextran, L-glutamine, fetal bovine serum, tobramycin, Hepes and rapamycin, Wherein the concentration of each component is as follows: chondroitin sulfate 25g / L, low molecular weight dextran 10g / L, L-glutamine 0.375mg / L, fetal bovine serum 5%, tobramycin 0.1g / L, Hepes9. 5g / L, rapamycin 1nM / L.

[0028] The preparation method of above-mentioned medium-term preservation solution of cornea comprises:

[0029] 1) According to the instructions of the MEM medium, take 9.4g of MEM medium powder and add 500ml of water for injection, dissolve and then filter and sterilize to prepare the MEM medium;

[0030] 2) Take 25g of chondroitin sulfate and add 200ml of water for injection, dissolve and filter to sterilize to prepare a chondroitin sulfate solution of 125g / L; take 10g of low-molecular dextran (average molecular weight 20000-40000kD) and add 100ml of wa...

Embodiment 2

[0035] In this embodiment, the concentration of each component in the medium-term corneal preservation solution is as follows: 21 g / L of chondroitin sulfate, 9 g / L of low molecular weight dextran, 0.372 mg / L of L-glutamine, and 3% of fetal bovine serum , Tobramycin 0.09g / L, Hepes9.2g / L, rapamycin 0.95nM / L. All the other are with embodiment 1.

Embodiment 3

[0037] In this embodiment, the concentration of each component in the medium-term corneal preservation solution is as follows: chondroitin sulfate 24g / L, low molecular weight dextran 11g / L, L-glutamine 0.378mg / L, fetal bovine serum 4% , Tobramycin 0.11g / L, Hepes9.8g / L, rapamycin 1.05nM / L.

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Abstract

The invention discloses a cornea metaphase preservation liquid and a preparation method thereof. The cornea metaphase preservation liquid is an MEM cell culture medium, which comprises the following components: chondroitin sulfate (20-26 g/L), low molecular dextran (8-12 g/L), L-glutamine (0.37-0.38 mg/L), fetal calf serum (2-5.5%), tobramycin (0.08-0.12 g/L), Hepes (9-10 g/L), and rapamycin (0.9-1.1 nM/L). The provided cornea metaphase preservation liquid can protect corneal endothelial cells, can avoid the phenomenon of necrosis and fusion of endothelial cells due to long time preservation, and prolongs the cornea preservation time more effectively.

Description

technical field [0001] The invention belongs to the technical field of corneal preservation solution preparation, and in particular relates to a corneal medium-term preservation solution and a preparation method thereof. Background technique [0002] In recent years, corneal disease has become the second leading cause of blindness in my country. However, as a curable eye disease, patients can regain their light by performing lamellar or penetrating keratoplasty. According to the preservation time of endothelial cell activity, corneal preservation can be divided into short-term, medium-term and long-term preservation. Mid-term preservation is a method of maintaining the activity of corneal endothelial cells for more than 4 days by keeping the preservation solution at 4°C. It has become the corneal preservation method widely used by eye banks in the world. [0003] The corneal endothelium is a single layer of cells inside the cornea, which can continuously drain the water in...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 李程刘昭麟吴洁丽刘祖国
Owner XIAMEN UNIV
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