9 results about "Photoacoustic microscopy" patented technology

The invention provides an unmarked photoacoustic microscopy blood flow direction and speed combined measurement method based on space-time cross correlation, which comprises the following steps: acquiring a two-dimensional structure image of a target tissue through a photoacoustic microscopy imaging platform, and carrying out image preprocessing and blood vessel enhancement on the two-dimensional structure image to obtain an enhanced tubular region; based on the enhanced tubular region, performing blood vessel segmentation, morphological optimization, center line extraction and direction calculation to obtain blood vessel direction information; path planning, scanning point sequence optimization and time sequence synchronization are carried out based on the blood vessel direction information, and a directional scanning path is obtained; a photoacoustic signal sequence is collected in the blood vessel direction, and space-time cross-correlation analysis is carried out; the blood flow direction and the blood flow speed are quantitatively calculated and visually displayed. According to the method, structural information guidance can be combined, rapid scanning in the blood vessel direction can be achieved, directional blood flow analysis is achieved through space-time cross-correlation, and the measuring speed and precision are remarkably improved.

Various embodiments may relate to a photoacoustic microscopy system. The photoacoustic microscopy system may include a flat optical component configured to focus a light beam emitted by a light source onto a focal point. The photoacoustic microscopy system may additionally include an acoustic receiving device configured to receive acoustic waves from the focal point. The photoacoustic microscopy system may further include an acoustic component configured to direct acoustic waves from the focal point to the acoustic receiving device. The photoacoustic microscopy system may also include an optical and acoustic combiner configured such that the light beam emitted by the light source is redirected within the optical and acoustic combiner towards the focal point, the optical and acoustic combiner further configured to allow the acoustic waves to pass through to the acoustic receiving device.

The invention discloses a differential ultrasonic transducer based on an acoustic differential method and a preparation method of the differential ultrasonic transducer. The differential ultrasonic transducer comprises a shell, a supporting layer, a backing layer, a bottom electrode layer, a top electrode layer and a piezoelectric unit layer. When external stress is received, acoustic differential signals with equal amplitude and opposite phases are output. The acoustic differential pair is creatively introduced into the piezoelectric unit layer, differential signals are generated from a physical source, external noise is effectively suppressed, and the signal-to-noise ratio is remarkably increased; the preparation method of the differential ultrasonic transducer is highly compatible with the process of a traditional transducer, and industrial production is easy to realize. The differential ultrasonic transducer is applied to a photoacoustic microscope system, and the imaging signal-to-noise ratio and contrast ratio in a complex electromagnetic and vibration environment can be remarkably improved under the condition of not depending on additional shielding or multiple averaging. The preparation process has the characteristics of high integration level and excellent anti-interference performance, and provides a reliable device basis for high-sensitivity photoacoustic imaging.

The invention relates to a photoacoustic microscopic imaging method and system. The method comprises the following steps: arranging a sample at a specified position; the spatial light modulator loads a phase diagram corresponding to the Gaussian light, scanning is carried out at a set rate, and an imaging result is obtained; selecting a region of interest according to the imaging result, and performing axial modulation imaging in the region of interest; in the axial modulation imaging process, the spatial light modulator loads 2N + 1 phase diagrams corresponding to non-sinusoidal modulation functions of different phases in sequence; phases corresponding to the 2N + 1 phase diagrams are as follows: N is the number of modulation frequencies; and reconstruction is carried out based on 2N + 1 one-dimensional photoacoustic signals obtained through axial modulation imaging scanning, and the photoacoustic signals after bandwidth expansion are obtained. According to the invention, the bandwidth of the photoacoustic signal can be expanded to GHz, an axial high-resolution imaging result is obtained, the data volume is reduced, the imaging time is shortened, and the imaging speed is improved.

This invention relates to the fields of nanomaterial applications and biomedical imaging technology, specifically to near-infrared absorbing gold nanoclusters, their preparation, and their application in photoacoustic microscopy. The near-infrared absorbing gold nanoclusters include Au. 60 NCs and Au 42 NCs, Au 60 The absorption peak of NCs is at 1090 nm, Au 42 Both NCs and Au exhibit strong absorption in the near-infrared region of 700–1700 nm, low absorption at 532 nm, and are water-soluble. 60 NCs and Au 42 NCs can be applied to photoacoustic microscopy, Au 60 NCs are excited using a dual-wavelength method at 532 nm / 1064 nm, with Au 42 NCs were excited using a dual-wavelength method at 532 nm / 808 nm; the Au 60 NCs and Au 42 NCs can be used in photoacoustic microscopy for high-resolution three-dimensional visualization and quantitative analysis of kidney microstructure.

The application discloses a kind of based on Monte Carlo model's light absorption coefficient accurate measurement method, system, equipment and medium, wherein the method includes constructing multimodal microscopic imaging system;Obtain the experimental photoacoustic data of multimodal microscopic imaging system, and the actual absorption light energy density is obtained by solving photoacoustic wave equation using finite element method;Given a hypothetical absorption coefficient, simulated calculation is carried out using Monte Carlo model to obtain simulated absorption light energy density;The deviation between actual absorption light energy density and simulated absorption light energy density is minimized by iterative least square method optimization, and the absolute light absorption coefficient is obtained. A multimodal microscopic imaging system is constructed by a fluorescence imaging microscope and a photoacoustic microscope, which combines the accuracy of FEM in solving photoacoustic wave equation and the rigorous modeling of MC in photon migration in biological tissues, and realizes the comprehensive quantitative reconstruction of optical absorption characteristics.

An adapter for a container that provides an integrated photoacoustic microscopy system that is capable of real time imaging of samples within the container. The adapter allows a researcher or investigator to evaluate a sample using the photoacoustic effect, with or without a microscope. The adapter comprises a support portion, a planar portion, and a tube. The support portion includes a channel formed therein, the channel including a first surface, a second surface opposite the first surface, and an intermediate surface positioned between the first surface and the second surface, the channel configured to receive the rim of the container such that the intermediate surface of the channel is positioned adjacent a top surface of the rim and the first surface and the second surface extend along opposite sides of the sidewall. The planar portion is coupled to the support portion and extends across the opening of the container. The tube is coupled to and extends from the planar portion, the tube defines a bore having an axis, the axis being oriented at an angle relative to the planar portion, the bore configured to receive a photoacoustic transducer for real time imaging of a sample within the container.

This invention relates to the field of optical microscopy imaging technology, specifically to a large depth-of-focus ultraviolet photoacoustic microscopy imaging system and method, comprising: an ultraviolet photoacoustic signal acquisition module to acquire spatiotemporal distribution data of ultraviolet excitation light and its corresponding photoacoustic response signal data; a spectral-polarization feature extraction module to extract quantitative multidimensional feature parameters related to tissue composition and structure; a cross-depth standard sample module to perform systematic imaging measurements on standard biological tissue samples at different depths to form a feature parameter dataset; and an image reconstruction module that, by linking the spatial distribution characteristics of the ultraviolet excitation light field with the physical propagation laws of photoacoustic signals at different depths, designs a hierarchical signal response adaptation link to match the signal transmission loss differences across tissue depths, and performs signal attenuation correction through a hierarchical dynamic neural radiation field algorithm to reconstruct a tissue microscopic image with large depth-of-focus characteristics. This solves the problems of generally short depths of focus and lack of standardized data support for cross-depth imaging in existing technologies.

A multimodal optical technique that can measure the mechanical, optical, and acoustical properties of the sample at microscopic resolution, which is based on the integration of a Brillouin microscope and a photoacoustic (PA) microscopy is provided. The multimodal technique not only can acquire co-registered Brillouin and PA signals of the sample but also allows us to utilize the sound speed measurements by PA to quantify the sample's refractive index, which is an essential property of the material and cannot be measured by either technique individually. We demonstrated the colocalization of Brillouin and time-resolved PA signals by measuring the interface of kerosene and 1% CuSO4 aqueous solution. In addition, we measured the refractive index of saline solutions with a precision of 0.003 and validated the result against published data. This multimodal modality could open new way for charactering biological cell and tissue in physiological and pathological conditions.