73 results about "Photoacoustic microscopy" patented technology

The invention discloses a rapid polarized light acoustic imaging device which comprises an excitation source, a light beam shaping module, a rapid polarization modulation module, an energy feedback module, a rapid scanning module and an acquisition processing module. And receiving photoacoustic signals corresponding to the exciting light in four different polarization states for calculation, and extracting anisotropy information of the sample. Compared with multiple times of scanning a sample or modulating the polarization state by rotating a half-wave plate, the problems of dislocation of multiple times of polarization acquisition points and false positive calculation are avoided, meanwhile, the time interval generated by continuously modulating the laser pulses is short, and energy fluctuation among the four pulses and errors caused by fluctuation are reduced. According to the invention, the limitation of the precision and speed of polarized light acoustic microscopic imaging is overcome, the microstructure characteristics in the target sample can be rapidly, accurately and highly sensitively detected, and the method has great application prospects in biomedical imaging and high-precision material detection.

The invention discloses a method for improving the axial resolution of photoacoustic microscopic imaging obtained based on a single-pixel frequency domain. The method comprises the following steps: 1) creating a Fourier substrate pattern with the spatial frequency of k in the axial direction; 2) respectively irradiating four Fourier substrate patterns with the spatial frequency of k and the initial phases of # imgabs0 #, # imgabs1 #, # imgabs2 # and # imgabs3 # to an imaging object, carrying out photoacoustic microscopic imaging, and acquiring data by using an ultrasonic transducer to obtain a response value of the ultrasonic transducer; 3) according to the obtained response value of the ultrasonic transducer, adopting a four-step phase shift method to obtain a Fourier coefficient corresponding to the spatial frequency k; and 4) gradually increasing the spatial frequency of the Fourier substrate pattern to the cut-off frequency of the objective lens, further obtaining Fourier coefficients corresponding to all spatial frequencies, obtaining a complete Fourier spectrum, and performing inverse Fourier transform to obtain a photoacoustic microscopic image after the axial resolution is increased. The method disclosed by the invention is expected to promote wider application of photoacoustic microscopic imaging in living cell and tissue imaging.

The invention discloses an unsupervised image registration method for a photoacoustic fast scanning system. The unsupervised image registration method comprises the following steps: acquiring data by using a fast scanning photoacoustic microscope; carrying out preprocessing steps of hooping, normalization, filtering, affine transformation and the like on the data to form a photoacoustic image data set; constructing a registration model based on a convolutional neural network and a space conversion module, fusing an attention mechanism to distribute weights for different pixels, adopting a two-stage training strategy, and introducing mutual information and a smooth item as a loss function; and predicting the registered photoacoustic image based on the training weight. According to the method, the registered image can be predicted in an extremely short time under the condition that a truth value is not input, an attention mechanism is introduced into the convolutional network, key factors are endowed with higher weights during feature extraction, the registration efficiency is effectively improved, mutual information is introduced as a loss function, the probability relation between pixel intensities is effectively modeled, and the registration accuracy is improved. And the similarity of the photoacoustic images acquired at different time points can be evaluated more accurately.

The invention relates to an optical device (1) for shaping and homogenizing a light beam (2), comprising: a source (3) of a light beam or pulse (2); a multimode optical fiber (7) having a core having a polygonal cross-section; an optical coupling module (5) for guiding the light beam (2) from the source (3) to the optical fiber (7); and an optical orientation module (13) that directs and focuses the formed and homogenized light beam or pulse (14) from the exit side (7 '') of the optical fiber (7) to the sample (19). Also disclosed is a system using the apparatus (1), which further comprises an ultrasonic receiving transducer (21) and an acquisition and processing unit for obtaining a photoacoustic image of a sample (19) in an optical or acoustic resolution photoacoustic microscope application and / or a photoacoustic tomography application.

The invention discloses a signal enhancement system and method based on passive high-frequency opto-acoustic / ultrasonic microscopic imaging. The system comprises a laser excitation module, an ultrasonic excitation detection module and a signal processing control module. According to the invention, the required target laser is excited through the laser excitation module, so that a good light source condition is provided for microscopic imaging; the method comprises the following steps: under ultrasonic microscopic imaging, exciting high-frequency ultrasonic waves through an opto-acoustic transducer, and gathering and enhancing the ultrasonic waves in an acoustic wave transmission space of the opto-acoustic transducer and a sample to be detected by utilizing an energy gathering device; under photoacoustic microscopic imaging, ultrasonic waves are excited on a detected sample by means of laser, and the laser and sound waves are reflected, focused and enhanced by an energy concentrator, so that the sensitivity and the signal-to-noise ratio of microscopic imaging are improved, and the detection efficiency of microscopic imaging is improved; and the signal processing control module controls the three-dimensional mobile platform to scan the tested sample through the controller to realize the integrity of ultrasonic signal acquisition of the tested sample, so that an ultrasonic image of the tested sample is generated.

The present invention discloses a photoacoustic microscopy imaging system based on a high-speed focuser. The photoacoustic microscopy imaging system includes a light source component, a single-mode fiber coupling component, a high-speed focuser component, a light beam scanning component, a reflective bracket, a signal acquisition component and a computer. The present invention adopts a mobile focusing design. During the photoacoustic imaging point scanning process, the displacement of the double-cemented lens is controlled by the movement of a fast linear motor, and then the focal position of the light beam is adjusted, so that during the scanning process, the focal plane at any position always coincides with the imaging surface. The present invention establishes an imaging method for automatically adjusting the focal plane by adopting a high-speed focuser, which effectively improves the image signal-to-noise ratio and resolution, and is low in price and easy to operate. The present invention adjusts the imaging in real time according to the photoacoustic signal fed back during the scanning process, and can perform photoacoustic microscopy imaging of any unknown surface, which has broad research prospects and application value.

The embodiment of the invention provides an image virtual dyeing method and device based on deep learning and a related assembly. The image virtual dyeing method comprises the following steps: utilizing a preprocessed photoacoustic microscopic image and a Hamp; e, performing iterative training on a pre-constructed virtual dyeing model by the dyeing image to obtain a trained virtual dyeing model, and performing virtual dyeing on the new photoacoustic microscopic image by using the trained virtual dyeing model to obtain Hamp; e, dyeing the image of the style (i.e., pseudo Hamp; e, dyeing the image). According to the embodiment of the invention, the image segmentation module is embedded in the generator to help the generator to distinguish cytoplasm and cell nucleus, so that the virtual staining model is constrained, and the generator can better generate a Hamp conforming result; e, dyeing the morphological characteristics of the image to ensure the generated pseudo Hamp; the E dyeing image retains more useful cell nucleus information, and the pre-constructed virtual dyeing model reduces noise in the learning process and effectively prevents the problem of overfitting due to the fact that an image segmentation module is embedded in a generator.

Disclosed in the present invention is an image enhancement method based on photoacoustic microscopy imaging. The method comprises: executing multiple photoacoustic microscopy scannings on a target object to obtain three-dimensional data of the target, and obtaining scanning results of the target on a time axis by means of continuous scanning imaging; before performing a projection operation on a single imaging result, for the three-dimensional data, within a set search range, combining signals of adjacent areas in space dimension; and for the scanning results on the time axis, within a set search range, combining signals of adjacent areas in time dimension, so as to obtain an enhanced image. The present invention effectively improves the image signal-to-noise ratio of a photoacoustic microscopy system, and improves the calculation efficiency.

A light irradiation apparatus focuses light on a surface or an inside of an object to observe or process the object, and includes a laser light source, a lens, a lens, a mask, a wave plate, and a lens. The mask is a multi-ring mask for spatially intensity-modulating input light in a beam cross-section and outputting modulated light, and includes a plurality of ring-shaped light-shielding areas provided around a center position, and a transmitting area provided between two adjacent light-shielding areas out of the plurality of light-shielding areas. A radial width of each of the two adjacent light-shielding areas out of the plurality of light-shielding areas is larger than a radial width of the transmitting area provided between the two light-shielding areas.

The invention discloses a photoacoustic microscopic imaging device and method based on photoacoustic confocal multi-depth polymerization. The imaging device comprises a laser emission module, a photoacoustic confocal multi-depth configuration module and a system control and signal processing module. Light beams emitted by the laser emission module form a plurality of optical focuses at different depth positions on a biological sample through the photoacoustic confocal multi-depth configuration module, and a multi-source photoacoustic signal is excited; the combined structure of the hollow transducer and the photoacoustic coupler enables multiple paths of photoacoustic signals to realize photoacoustic confocal, the detection sensitivity and the imaging signal-to-noise ratio are improved, the structure is compact, the operation is flexible, and the practicability in related fields is improved; according to the method, multiple paths of signals are independently reconstructed and imaged, and a high-resolution and high-contrast imaging result is obtained through focus information extraction and fusion, so that the depth of field of photoacoustic imaging can be improved, and high-sensitivity uniform high-resolution imaging can be realized in a relatively large depth range.

A computer-implemented method (530), system (100, 201) and computer readable medium for generating a slide-free histological image in the form of a reconstructed ultraviolet-based autofluorescence microscopy (UV-AutoM) image from a plurality of speckle-illuminated low-resolution images. A computer implemented method (1150), system (100, 201) and computer readable medium for generating a slide-free histological image in the form of a pseudo-hematoxylin and cosin (H&E) stained image (1120) from a grayscale input image such as a UV-AutoM image or a ultraviolet-based photoacoustic microscopy (UV-PAM) image (1110).

The invention relates to an optical device (1) for shaping and homogenizing a light beam (2), comprising a light source (3) of a light beam or light pulses (2), a multimode optical fiber (7) having a core with a polygonal cross section, an optical coupling module (5) for guiding the light beam (2) from the light source (3) into the fiber (7), and a light direction module (13) for guiding and focusing the homogenized light beam or light pulses (14) from the outlet side (7") of the optical fiber (7) towards a sample (19). Also disclosed is a system using the device (1), further comprising an ultrasonic receiving transducer (21) and an acquisition and processing unit, for acquiring photoacoustic images of a sample (19) in optical or acoustic resolution photoacoustic microscopy and / or photoacoustic tomography applications.

A radiotherapy system for treatment of skin includes a confocal microscopy imaging component and a photoacoustic microscopy imaging component to capture images of the skin of a patient at more than one depth. A processor combines images to produce a fused model for the region of interest, generates a plan for radiotherapy treatment based on the fused model, and controls the radiotherapy component for carrying out the radiotherapy treatment.

The invention provides an unmarked photoacoustic microscopy blood flow direction and speed combined measurement method based on space-time cross correlation, which comprises the following steps: acquiring a two-dimensional structure image of a target tissue through a photoacoustic microscopy imaging platform, and carrying out image preprocessing and blood vessel enhancement on the two-dimensional structure image to obtain an enhanced tubular region; based on the enhanced tubular region, performing blood vessel segmentation, morphological optimization, center line extraction and direction calculation to obtain blood vessel direction information; path planning, scanning point sequence optimization and time sequence synchronization are carried out based on the blood vessel direction information, and a directional scanning path is obtained; a photoacoustic signal sequence is collected in the blood vessel direction, and space-time cross-correlation analysis is carried out; the blood flow direction and the blood flow speed are quantitatively calculated and visually displayed. According to the method, structural information guidance can be combined, rapid scanning in the blood vessel direction can be achieved, directional blood flow analysis is achieved through space-time cross-correlation, and the measuring speed and precision are remarkably improved.

The invention belongs to the field of optical microscopic imaging, and provides a bimodal optical microscopic imaging device for detecting structures and components of traditional Chinese medicinal materials, which is based on the characteristics that the traditional Chinese medicinal materials can present different internal structure states and different characteristic component contents due to different types, different producing areas and different processing technologies. By combining an all-optical non-contact photoacoustic microscopy remote sensing imaging technology with a fluorescence microscopy technology, non-contact structure and component detection of traditional Chinese medicinal materials is realized, and meanwhile, data processing and analysis are performed in combination with an intelligent algorithm, so that rapid nondestructive quality identification of the traditional Chinese medicinal materials is realized.

This invention discloses a super-resolution photoacoustic microscopy imaging method based on a deep neural network, comprising: acquiring two sets of photoacoustic signal data at high and low resolution using an optical resolution photoacoustic microscopy imaging system; reconstructing images from the two sets of acquired photoacoustic signal data; preprocessing the two sets of reconstructed original photoacoustic images to form an input sample set; building and training a deep neural network model, specifically a DenseRes-SRGAN adversarial neural network architecture optimized based on photoacoustic images; and inputting the acquired high and low resolution images into the trained deep neural network to obtain super-resolution results. The high and low resolution sampling of this invention does not skip information within the field of view. Compared with sparse data, the representative pixels are a weighted representation of each small pixel in a large step scan mode, thereby reproducing low-scale information that cannot be resolved by low resolution and large focal diameter. Compared with high-resolution reconstruction of sparse data, it has improved reconstruction generalization ability and realism.

The present invention presents a novel photoacoustic or optoacoustic microscopy structure capable of solving the problem wherein accessing a target area when scanning various recessed areas of a human body is difficult due to the large physical size occupied by a scanning head, that is, the large footprint problem of the scanning head in conventional mechanical scanning-type photoacoustic or optoacoustic microscopy systems. A photoacoustic microscope presented by the present invention makes it possible to significantly reduce the footprint compared to the prior art when implementing a relevant device, and thus can be variously applied to not only a handle-type, that is, a handheld probe implemented so that a user can freely image any desired area by holding the device directly by hand, but also to a miniature probe used to visualize an area of interest in an invasive manner accompanying surgery or a non-invasive manner, and an endoscope device used to diagnose suspected disease areas such as the gastrointestinal tract or blood vessels.

Various embodiments may relate to a photoacoustic microscopy system. The photoacoustic microscopy system may include a flat optical component configured to focus a light beam emitted by a light source onto a focal point. The photoacoustic microscopy system may additionally include an acoustic receiving device configured to receive acoustic waves from the focal point. The photoacoustic microscopy system may further include an acoustic component configured to direct acoustic waves from the focal point to the acoustic receiving device. The photoacoustic microscopy system may also include an optical and acoustic combiner configured such that the light beam emitted by the light source is redirected within the optical and acoustic combiner towards the focal point, the optical and acoustic combiner further configured to allow the acoustic waves to pass through to the acoustic receiving device.

The invention discloses a differential ultrasonic transducer based on an acoustic differential method and a preparation method of the differential ultrasonic transducer. The differential ultrasonic transducer comprises a shell, a supporting layer, a backing layer, a bottom electrode layer, a top electrode layer and a piezoelectric unit layer. When external stress is received, acoustic differential signals with equal amplitude and opposite phases are output. The acoustic differential pair is creatively introduced into the piezoelectric unit layer, differential signals are generated from a physical source, external noise is effectively suppressed, and the signal-to-noise ratio is remarkably increased; the preparation method of the differential ultrasonic transducer is highly compatible with the process of a traditional transducer, and industrial production is easy to realize. The differential ultrasonic transducer is applied to a photoacoustic microscope system, and the imaging signal-to-noise ratio and contrast ratio in a complex electromagnetic and vibration environment can be remarkably improved under the condition of not depending on additional shielding or multiple averaging. The preparation process has the characteristics of high integration level and excellent anti-interference performance, and provides a reliable device basis for high-sensitivity photoacoustic imaging.

The invention belongs to the related technical field of imaging technology, and discloses a multi-view-angle-based photoacoustic microscopic imaging system, which comprises a two-dimensional scanning module, a view angle selection module, a signal detection module and a three-dimensional reconstruction module, and is characterized in that the two-dimensional scanning module enables a light beam to carry out multiple XY plane two-dimensional scanning on a sample; the visual angle selection module adjusts the visual angle of each two-dimensional scanning to enable the angle of each two-dimensional scanning to be different, the visual angle is the direction in which the light beam enters the sample, and the visual angle of each two-dimensional scanning is perpendicular to the signal detection direction; the signal detection module is located on the side face of the sample and receives ultrasonic signals in the detection direction, the detection direction is parallel to the XY plane, and the ultrasonic signals are ultrasonic signals generated by the sample under light beam excitation; and the three-dimensional reconstruction module performs three-dimensional reconstruction according to the ultrasonic signals at different visual angles to obtain a three-dimensional body structure of the sample. According to the system, the axial resolution of photoacoustic microscopy can be improved in a simpler mode, and scanning and three-dimensional reconstruction can be rapidly completed.

The present application relates to a kind of high-speed scanning photoacoustic image input device and method thereof, make photoacoustic microscope high-speed scanning simultaneously, receive the input of ultrasonic wave signal and convert into three-dimensional image, so high-resolution three-dimensional image about detection object body can be generated at high speed.The high-speed scanning photoacoustic image input device of the present application, comprising: photoacoustic transceiver, by laser generation part towards detection object body output laser pulse output, receive the ultrasonic wave image signal emitted from detection object body;Analog-digital conversion unit, receive the input of ultrasonic wave image signal, and convert into digital image signal;Main control unit, receive the input of digital image signal, generate the ultrasonic wave scanning three-dimensional image information about detection object body;Trigger control unit, receive the input of photoacoustic probe motion information, generate the scanning trigger corresponding to motion information, and main control unit is sequentially synthesized with the image corresponding to ultrasonic wave image signal according to scan line unit, generate the image about detection object body.

The present invention relates to the field of microscopic imaging technology, and in particular provides a limited-viewing-angle corrected photoacoustic microscopy imaging system based on three-beam interference excitation. The limited-viewing-angle corrected photoacoustic microscopy imaging system based on three-beam interference excitation includes a control circuit, a fixing component, a pulsed laser, a light field modulation component, an optical path lens component, an ultrasonic probe, and a three-dimensional scanning platform. The present invention utilizes a light field modulation component to modulate the excitation light field in the photoacoustic microscopy imaging system, and uses a striped light field formed by three-beam interference to excite a photoacoustic signal. Because the striped light field can effectively destroy the spatial coherence between ultrasonic wave sources excited by the photoacoustic effect, the limited-viewing-angle defect caused by coherent decomposition in the three-dimensional reconstructed image is eliminated without affecting system performance indicators such as imaging speed and imaging depth.

The invention relates to a photoacoustic microscopic imaging method and system. The method comprises the following steps: arranging a sample at a specified position; the spatial light modulator loads a phase diagram corresponding to the Gaussian light, scanning is carried out at a set rate, and an imaging result is obtained; selecting a region of interest according to the imaging result, and performing axial modulation imaging in the region of interest; in the axial modulation imaging process, the spatial light modulator loads 2N + 1 phase diagrams corresponding to non-sinusoidal modulation functions of different phases in sequence; phases corresponding to the 2N + 1 phase diagrams are as follows: N is the number of modulation frequencies; and reconstruction is carried out based on 2N + 1 one-dimensional photoacoustic signals obtained through axial modulation imaging scanning, and the photoacoustic signals after bandwidth expansion are obtained. According to the invention, the bandwidth of the photoacoustic signal can be expanded to GHz, an axial high-resolution imaging result is obtained, the data volume is reduced, the imaging time is shortened, and the imaging speed is improved.

A system for calculating a Grüneisen parameter of a target tissue is disclosed. The system comprises a first light source configured to emit a first light signal to the target tissue, thereby generating an acoustic signal in the target tissue. The system further comprises a second light source configured to emit a second light signal to the target tissue, which interacts with the acoustic signal, (e.g., in the manner of Brillouin scattering) to generate a backscattered light signal. The system further comprise a light sensor configured to detect the backscattered light signal and a processor configured to receive the backscattered light signal from the light sensor and calculate the Grüneisen parameter of the target tissue based on the backscattered response signal.

The present invention discloses a method for establishing a photoacoustic imaging model for monitoring acid-base imbalance, which belongs to the field of acid-base imbalance detection and includes the following steps: S1: establishing a lactic acidosis model in an experimental animal; the lactic acidosis model includes: infusing the experimental animal in the perioperative period with a lactic acid solution of different concentrations; S2: performing a blood gas analysis on the experimental animal in the lactic acidosis model; wherein, after the lactic acid solution is pumped intravenously, the experimental animal is subjected to an arterial puncture and a blood gas analysis is performed to measure the acid-base abnormality of the experimental animal; S3: using a photoacoustic microscope to monitor the intracranial blood vessels of the experimental animal; S4: filtering and binarizing the photoacoustic image, and automatically performing quantitative analysis on the blood vessel diameter, blood vessel length, and blood vessel density, monitoring the dynamic changes in pH, and obtaining the relationship between the changes in intracranial blood vessel diameter and pH. The present invention can achieve non-invasive, in vivo, real-time monitoring of acidosis, alkalosis, and brain function metabolism.

This invention relates to the fields of nanomaterial applications and biomedical imaging technology, specifically to near-infrared absorbing gold nanoclusters, their preparation, and their application in photoacoustic microscopy. The near-infrared absorbing gold nanoclusters include Au. 60 NCs and Au 42 NCs, Au 60 The absorption peak of NCs is at 1090 nm, Au 42 Both NCs and Au exhibit strong absorption in the near-infrared region of 700–1700 nm, low absorption at 532 nm, and are water-soluble. 60 NCs and Au 42 NCs can be applied to photoacoustic microscopy, Au 60 NCs are excited using a dual-wavelength method at 532 nm / 1064 nm, with Au 42 NCs were excited using a dual-wavelength method at 532 nm / 808 nm; the Au 60 NCs and Au 42 NCs can be used in photoacoustic microscopy for high-resolution three-dimensional visualization and quantitative analysis of kidney microstructure.

A system for calculating a Grüneisen parameter of a target tissue is disclosed. The system comprises a first light source configured to emit a first light signal to the target tissue, thereby generating an acoustic signal in the target tissue. The system further comprises a second light source configured to emit a second light signal to the target tissue, which interacts with the acoustic signal, (e.g., in the manner of Brillouin scattering) to generate a backscattered light signal. The system further comprise a light sensor configured to detect the backscattered light signal and a processor configured to receive the backscattered light signal from the light sensor and calculate the Grüneisen parameter of the target tissue based on the backscattered response signal.

The present invention discloses a multi-scale photoacoustic microscopy method based on single-pixel imaging, comprising: 1) generating a series of stripe lights of different spatial frequencies in the XY two-dimensional plane to illuminate the sample, applying a phase shift to the stripe lights of each spatial frequency using a phase shifting method, and acquiring the photoacoustic signals at each phase shift using a single ultrasonic probe; 2) calculating Fourier spectrum coefficients based on the photoacoustic signals at each spatial frequency, modifying the Fourier spectrum coefficients by introducing the impulse response function of the ultrasonic probe, and then reconstructing the photoacoustic image based on the modified Fourier spectrum coefficients through an inverse Fourier transform based on a simplified point spread function (PSF); and 3) controlling the acquisition of the Fourier spectrum coefficients by controlling the spatial frequency of the stripe light, thereby achieving multi-scale imaging. The present method solves the problem that traditional photoacoustic microscopy, due to its fixed resolution, can only image at a single scale and cannot fully utilize multi-scale information.

An optical-resolution photoacoustic microscopy (OR-PAM) system for visualizing water content deep in biological tissue uses an all-fiber 1930-nm hybrid optical parametrically-oscillating emitter. The emitter includes a tunable laser source whose output is amplified by a first erbium-doped fiber amplifier (EDFA). The output of the first amplifier is modulated with a Mach-Zehnder amplitude modulator that receives an RF signal with a nanosecond pulse width and a multiple kilohertz repetition rate. A second EDFA further amplifies the signal and passes it to a fiber circulator that in turn delivers it to a 1950 / 1550 mm fiber wavelength-division-multiplexing coupler WDM. The coupler introduces the signal to a cavity that includes a spool of highly nonlinear fiber and a Thulium-doped fiber amplifier TDFA. From the TDFA the signal reaches a 50 / 50 fiber coupler that sends part to a second output TDFA and guides part back to the cavity through a port of the WDM.

A handheld device for photoacoustic microscopy. The handheld device includes an optical assembly, a light beam scanner and a transducer unit. The optical assembly is arranged to provide a light beam. The light beam scanner includes a reflector arranged to reflect the light beam from the optical assembly to provide a reflected light beam to an object, a first drive mechanism operable to move the reflector relative to the optical assembly to move the reflected light beam relative to the object, and a second drive mechanism operable to move the reflector relative to the optical assembly to move the reflected light beam relative to the object. The transducer unit is arranged to detect photoacoustic signals emitted by the object in response to receiving the reflected light beam.