Skin care composition, preparation method and skin care cream

Abstract

The invention discloses a skin care composition, a preparation method and skin care cream, and relates to the technical field of skin care products. Raw materials of the skin care composition comprise decarboxylated carnosine, a plant fermentation extract, a traditional Chinese medicine composition and the like, the traditional Chinese medicine composition is an extract of centella asiatica and phyllanthus emblica, and plant raw materials of the plant fermentation extract comprise bulbs of lilac and roots, stems and leaves of panax plants. By compounding the decarboxylated carnosine, the plant fermentation extract and the specific traditional Chinese medicine composition, the effects of inhibiting melanogenesis, regulating the skin state and promoting repair are synergistically exerted, and meanwhile, by adopting a step-by-step fermentation and oil-water phase split preparation process, the skin care cream developed by the invention has the effects of keeping skin elasticity and stimulating skin immunity, and the skin care cream has a good application prospect. The skin state can be obviously improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care, in particular to a skin care composition, a preparation method and a skin care cream. BACKGROUND

[0002] With the improvement of people's living standards, having white and healthy skin has become a fashion trend, and the skin is accelerated in aging by the effect of environmental pollutants, so that the skin gradually loses luster and elasticity, and the microecological balance on the surface of the skin is broken, so that the immune repair ability of the skin surface is poor, thereby causing skin inflammation, swelling, itching and other problems. Therefore, how to effectively inhibit the production and deposition of skin melanin, inhibit the breeding of pathogenic bacteria on the skin, and promote the improvement of the self-repairing ability of the skin is of great significance for the research and development of existing skin care products. With people's increasing awareness of the side effects of chemical synthetic antioxidants, natural plant antioxidants are more and more favored by consumers due to their green, natural and side effect-free advantages, so the research and development of plant source antioxidant whitening skin factors have great significance. SUMMARY

[0003] The main purpose of the present application is to develop a skin care composition containing plant fermentation extract and traditional Chinese medicine composition synergistic effect and apply it to a skin care cream. The skin care cream can resist skin aging through multiple mechanisms such as antioxidant and collagen synthesis promotion, thereby protecting cell vitality from the root and maintaining the young state of the skin.

[0004] To achieve the above-mentioned purpose, the present application provides a skin care composition, and the technical scheme is as follows: the skin care composition comprises the following raw materials in parts by weight: decarboxylated myo-inositol: 5-15 parts; plant fermentation extract: 8-25 parts; traditional Chinese medicine composition: 3-6 parts; emulsifier: 1-2 parts; emollient: 20-30 parts; thickening agent: 3-10 parts; deionized water: 40-80 parts; the traditional Chinese medicine composition is the extract of Centella asiatica and Phyllanthus emblica; the plant raw material of the plant fermentation extract comprises the bulb of the plant of Bulbophyllum and the root, stem and leaf of the plant of Panax.

[0005] Further, the present application further provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps: crushing Centella asiatica and Phyllanthus emblica into powder, adding the powder into an extraction solvent, and extracting at 50-80 DEG C for 10-20 hours to obtain the traditional Chinese medicine composition.

[0006] Further, the present application further provides that the extraction solvent is at least one selected from the group consisting of coconut oil, olive oil, sweet almond oil, grape seed oil, jojoba oil, rose fruit oil, sesame oil, wheat germ oil and camellia seed oil; and / or the mass ratio of the powder raw material to the extraction solvent is 1: (3-10).

[0007] Furthermore, this application also proposes that the *Bulbophyllum* species are selected from one or more of *Bulbophyllum rotundifolium*, *Bulbophyllum dentata*, *Bulbophyllum denticulatum*, *Bulbophyllum aromaticum*, *Bulbophyllum macrobrachium*, *Bulbophyllum longiflorum*, and *Bulbophyllum rubrum*; and / or that the *Panax* species are selected from one or more of ginseng, American ginseng, Panax notoginseng, Panax quinquefolius, and Panax quinquefolius.

[0008] Furthermore, this application also proposes that the preparation process of the plant fermentation extract includes the following steps: T1, after crushing the bulbs of *Bulbus orientalis* and the roots, stems and leaves of *Panax ginseng* plants into a material-to-liquid ratio of 1:(8~15), mix with water, crush to obtain a slurry, sterilize, extract by ultrasonication, centrifuge to obtain a supernatant and a lower precipitate, and collect the supernatant to obtain an extract; T2, collect the lower precipitate from step S1, add *Aspergillus niger* powder, carbon source, nitrogen source and deionized water, and carry out a primary fermentation; then add yeast and lactobacillus for a secondary fermentation, after the fermentation is completed, add the extract obtained in step T1, add organic acid solution, react, centrifuge, concentrate, and obtain the plant fermentation extract.

[0009] Furthermore, this application also proposes that, in step T1, the mass percentage of bulbs of *Bulbus bulgaricus* in the plant raw materials of the plant fermentation extract is 55wt% to 75wt%; and the mass percentage of roots of *Panax* plants in the plant raw materials of the plant fermentation extract is 8wt% to 14wt%.

[0010] Furthermore, this application also proposes that, in step T2, the nitrogen source is peptone and the carbon source is pectin; and / or, in step T2, the weight ratio of the lower precipitate, carbon source, nitrogen source and deionized water is (10~15):(2~4):(0.5~0.9):(14~30); and / or, in step T2, the organic acid solution is selected from at least one of citric acid, lactic acid, formic acid, acetic acid, malic acid and tartaric acid; and / or, in step S4, the amount of organic acid solution used is 0.05 g / L~0.6 g / L, the reaction time is 24 h~72 h, and the concentration ratio is 40 times~60 times.

[0011] Furthermore, this application also proposes that, in step T2, during the primary fermentation, aerobic fermentation is carried out at a fermentation temperature of 28℃~36℃ and a fermentation time of 4d~8d; and / or, in step T2, during the secondary fermentation, anaerobic fermentation is carried out, with the pH value adjusted to 4.5~5.5, the fermentation temperature at 25~35℃, and the time being 1d~2d.

[0012] Furthermore, this application also proposes that the emulsifier is selected from one or more of nonionic emulsifiers and anionic emulsifiers; and / or, the thickener is selected from one or more of natural macromolecular polysaccharide polymers; and / or, the emollient is selected from one or more of natural polyols.

[0013] Furthermore, this application also proposes a method for preparing the skin care composition comprising the following steps: S1. Disperse the aqueous phase raw material in half of the deionized water, stir and heat to 60℃~80℃, keep warm and stir for 10min~25min; the aqueous phase raw material includes: plant fermentation extract and emollient; S2. Disperse the oil phase raw materials in the other half of the deionized water, stir and heat to 90℃~110℃, and keep warm and stir for 20min~35min; the oil phase raw materials include: decarboxylated carnosine, traditional Chinese medicine composition, and thickener; S3. Add the dispersed oil phase raw material from step S2 to the dispersed aqueous phase raw material from step S1, add emulsifier, stir for 10 min to 35 min, homogenize for 10 min to 15 min, and complete the preparation.

[0014] The present invention also proposes a skin care cream, wherein the skin care cream is applied using the skin care composition.

[0015] The technical solution of this invention designs a skin care composition and its preparation method. By compounding decarboxylated carnosine, plant fermentation extracts and a specific traditional Chinese medicine composition, it synergistically exerts the effects of inhibiting melanin production, regulating skin condition and promoting repair. At the same time, the preparation process adopts a stepwise fermentation and oil-water phase separation preparation process. The skin care cream developed by this invention using the above skin care composition has the effects of maintaining skin elasticity and stimulating skin immunity, and can significantly improve skin condition. Detailed Implementation

[0016] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0017] It should be noted that if the embodiments of the present invention involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a specific posture. If the specific posture changes, the directional indicators will also change accordingly.

[0018] Furthermore, if the embodiments of this invention involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the use of "and / or" or "and / or" throughout the text includes three parallel solutions. For example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.

[0019] As people's living standards improve, achieving fair and healthy skin has become increasingly fashionable. However, environmental pollutants accelerate cell aging, causing skin to gradually lose its luster and elasticity. Furthermore, the imbalance of the skin's microecological environment is disrupted, weakening the skin's immune repair capabilities and leading to problems such as inflammation, redness, and itching. Therefore, effectively inhibiting melanin production and deposition, suppressing the growth of pathogenic bacteria, and promoting the skin's self-repair capabilities are crucial for the development of existing skincare products. With growing awareness of the side effects of chemically synthesized antioxidants, naturally derived plant antioxidants, with their green, natural, and side-effect-free advantages, are gaining increasing popularity among consumers, making the research and development of plant-based antioxidant whitening skincare factors of great significance.

[0020] To address the aforementioned technical challenges, a skin care composition containing plant fermentation extracts and traditional Chinese medicine ingredients was developed. This composition can combat skin aging through multiple mechanisms, including anti-oxidation and promoting collagen synthesis, thereby protecting cell vitality from the root and maintaining youthful skin.

[0021] This invention proposes a skin care composition, the technical solution of which is as follows: The skin care composition comprises the following raw materials in parts by weight: decarboxylated carnosine: 5 to 15 parts; plant fermentation extract: 8 to 25 parts; traditional Chinese medicine composition: 3 to 6 parts; emulsifier: 1 to 2 parts; emollient: 20 to 30 parts; thickener: 3 to 10 parts; deionized water: 40 to 80 parts; the traditional Chinese medicine composition is an extract of Centella asiatica and Phyllanthus emblica; the plant raw materials of the plant fermentation extract include the bulb of Bulbus breviscapus and the roots, stems and leaves of plants of the genus Panax.

[0022] It should be noted that decarboxylated carnosine reduces oxidative stress damage to skin cells by scavenging free radicals. The bulb extract of *Bulbus buergerianum*, after fermentation, produces β-glucan, which binds to receptors on the skin surface, activating the immune response and regulating the balance of the epidermal flora. Ginsenoside Rb1, released during the fermentation of ginseng roots, stems, and leaves, can enhance keratinocyte vitality and promote barrier repair. The asiaticoside in *Centella asiatica* extract forms a complex with ellagic acid in *Amla spp.*, reducing tyrosinase catalytic activity and blocking the melanin production pathway. Through the above technical solutions, this application can simultaneously achieve the triple effects of inhibiting melanin production, regulating the balance of the skin's surface flora, and promoting barrier function repair. The synergistic effect of decarboxylated carnosine and plant fermentation products reduces inflammatory responses caused by oxidative damage; the *Centella asiatica*-*Amla spp.* composition reduces tyrosinase activity through multi-target action; and the organic acids produced during fermentation maintain the skin's slightly acidic environment, inhibiting the proliferation of pathogenic bacteria. The combination of naturally derived ingredients avoids the irritation of sensitive skin by chemically synthesized substances, making it suitable for long-term skincare use. Oil-soluble extraction processes improve the bioavailability of active ingredients, while fermentation technology transforms macromolecules that are more easily absorbed by the skin.

[0023] In one embodiment, the preparation method of the traditional Chinese medicine composition includes the following steps: pulverizing Centella asiatica and Phyllanthus emblica into powder, adding them to an extraction solvent, and extracting at 50℃~80℃ for 10h~20h to obtain the traditional Chinese medicine composition; wherein, the weight ratio of Centella asiatica and Phyllanthus emblica is 1:(0.15~0.4).

[0024] It should be noted that this invention ruptures the cell wall structure, exposing the internal active substances of the traditional Chinese medicine composition. In a selected extraction solvent, the powder and solvent form a solid-liquid two-phase system, with solvent molecules penetrating and diffusing into the plant cells to dissolve the target components. When the temperature is controlled between 50℃ and 80℃, the solvent viscosity decreases and molecular motion accelerates, promoting an increased mass transfer rate. Simultaneously, this temperature range is below the decomposition critical point of polyphenols. A stable concentration gradient is established during the 10-20 hour extraction process, allowing the active ingredients to continuously dissolve into the solvent phase. Flavonoids and saponins reach their maximum leaching rate within this time range, achieving highly efficient extraction of antioxidant components from Centella asiatica and Phyllanthus emblica.

[0025] In one embodiment, the extraction solvent is selected from at least one of coconut oil, olive oil, sweet almond oil, grape seed oil, jojoba oil, rosehip oil, sesame oil, wheat germ oil, and camellia seed oil; and / or, the mass ratio of the powdered raw material to the extraction solvent is 1:(3~10).

[0026] Understandably, this application uses natural plant oils containing unsaturated fatty acids for extraction. These plant oils are polarly compatible with the fat-soluble active ingredients, which can reduce the oxidative decomposition of polyphenols. The solvent fully wets the plant powder to form a mass transfer interface, promoting the dissolution of flavonoids and saponins, while avoiding excessive solvent leading to increased energy consumption in the concentration process.

[0027] In one embodiment, the Bulbus orchid is selected from one or more of the following: Bulbus orchid rotundifolia, Bulbus orchid serratedus, Bulbus orchid dense-flowered, Bulbus orchid aromaticus, Bulbus orchid dauricum, Bulbus orchid salvia, and Bulbus orchid rubrotinctum.

[0028] In one embodiment, the ginseng plant is selected from one or more of ginseng, American ginseng, Panax notoginseng, Panax japonicus, and Panax quinquefolius.

[0029] In one embodiment, the preparation process of the plant fermentation extract includes the following steps: T1. After crushing the bulbs of *Bulbus buergerianum* and the roots, stems and leaves of *Panax ginseng* plants, mix them with water at a ratio of 1: (8~15), crush to obtain a slurry, sterilize, extract by ultrasonication, centrifuge to obtain the supernatant and the lower precipitate, and collect the supernatant to obtain the extract. T2. Collect the lower precipitate from step S1, add Aspergillus niger powder, carbon source, nitrogen source and deionized water, and carry out primary fermentation; then add yeast and lactobacillus for secondary fermentation. After fermentation, add the extract obtained in step T1, add organic acid solution, react, centrifuge, concentrate, and obtain plant fermentation extract.

[0030] It should be noted that the combination of physical pulverization and ultrasonic treatment effectively breaks down plant cell walls, releasing active substances such as polysaccharides and saponins. After centrifugation, the supernatant is rich in small-molecule active ingredients, while the lower precipitate contains incompletely decomposed plant fibers and macromolecules. During the fermentation stage, the primary fermentation utilizes cellulase secreted by Aspergillus niger to decompose the fibrous matrix in the lower precipitate, generating soluble oligosaccharides. The secondary fermentation, through the sugar metabolism of yeast and the organic acid synthesis of lactobacilli, produces active substances such as amino acids and short-chain fatty acids. Finally, the extract and fermentation products are mixed, forming a stable complex system under the action of organic acids, effectively preventing the oxidative deactivation of active ingredients.

[0031] Through the above technical solution, this invention achieves efficient conversion of plant fibers through staged directional fermentation, and generates diverse active ingredients through the differentiated metabolism of complex microbial strains. Simultaneously, the precipitate after centrifugation is reintroduced into the fermentation system, avoiding resource waste. This technical solution improves the extraction efficiency of plant active ingredients while maintaining the stability of heat-sensitive components such as ginsenosides. The staged fermentation process simultaneously increases antioxidant and anti-inflammatory components, further enhancing the synergistic inhibition of oxidase activity. The resulting plant fermentation extract exhibits multi-dimensional synergistic effects in whitening, anti-inflammation, and skin repair.

[0032] In one embodiment, in step T1, the mass percentage of the bulb of *Bulbus bulgaricus* in the plant raw material of the plant fermentation extract is 55wt%~75wt%; and the mass percentage of the root of *Panax* in the plant raw material of the plant fermentation extract is 8wt%~14wt%.

[0033] It should be noted that the high proportion of *Bulbus buergerianum* bulbs forms the basic supporting structure of the fermentation system, with its polysaccharide components forming a network matrix that encapsulates the microbial cells, ensuring environmental stability during fermentation. The moderate proportion of *Panax ginseng* roots activates microbial metabolic pathways by releasing saponins, promoting the synthesis of antioxidant peptides and anti-inflammatory factors. The colloidal properties of *Bulbus buergerianum* bulbs buffer pH fluctuations during fermentation, while the active ingredients in *Panax ginseng* roots precisely regulate the synergistic effect of *Aspergillus niger* and lactic acid bacteria, ultimately achieving efficient conversion and stable release of active ingredients.

[0034] In one embodiment, in step T2, the nitrogen source is peptone and the carbon source is pectin; In one embodiment, in step T2, the weight ratio of the lower precipitate, carbon source, nitrogen source, and deionized water is (10~15):(2~4):(0.5~0.9):(14~30). In one embodiment, in step T2, the organic acid solution is selected from at least one of citric acid, lactic acid, formic acid, acetic acid, malic acid, and tartaric acid; In one embodiment, in step S4, the amount of organic acid solution used is 0.05 g / L to 0.6 g / L, the reaction time is 24 h to 72 h, and the concentration ratio is 40 to 60 times.

[0035] In one embodiment, during step T2, the primary fermentation is carried out aerobic fermentation at a temperature of 28°C to 36°C for 4 to 8 days; and / or, during step T2, the secondary fermentation is carried out anaerobic fermentation at a pH of 4.5 to 5.5 at a temperature of 25 to 35°C for 1 to 2 days.

[0036] It should be noted that during the primary fermentation stage, maintaining a sufficient oxygen supply and moderate temperature allows Aspergillus niger to fully secrete cellulase, effectively breaking down the cell wall structure of the plant material and releasing intracellular active ingredients. Subsequently, the secondary fermentation stage creates an anaerobic environment and weakly acidic conditions, promoting the synergistic effect of yeast and lactobacillus, transforming macromolecules into small-molecule peptides and organic acids that are easily absorbed by the skin. Both fermentation stages avoid high-temperature zones to prevent the degradation of heat-sensitive components such as polyphenols.

[0037] In one embodiment, the emulsifier is selected from one or more of nonionic emulsifiers and anionic emulsifiers; In one embodiment, the thickener is selected from one or more natural macromolecular polysaccharide polymers; In one embodiment, the emollient is selected from one or more natural polyols. It should be noted that nonionic emulsifiers can be specifically achieved using polyglycerol esters or sucrose fatty acid esters, which reduce surface tension to form a stable droplet structure and avoid damaging the skin barrier function. Anionic emulsifiers can be specifically achieved using lecithin or sodium stearate, reducing the dissolving effect on the stratum corneum. Natural macromolecular polysaccharide polymers can be specifically achieved using xanthan gum or sodium hyaluronate, which enhance the retention ability of active ingredients on the skin surface. Natural polyols can be specifically achieved using glycerin or sorbitol, which achieve continuous moisturizing through the formation of a hydrogen bond network with water molecules via hydroxyl groups, while avoiding pore closure.

[0038] The present invention also provides a method for preparing the skin care composition, comprising the following steps: S1. Disperse the aqueous raw material in half of the deionized water, stir and heat to 60℃~80℃, and keep warm and stir for 10min~25min; the aqueous raw material includes: plant fermentation extract and emollient; S2. Disperse the oil phase raw material in the other half of the deionized water, stir and heat to 90℃~110℃, and keep warm and stir for 20min~35min; the oil phase raw material includes: decarboxylated carnosine, traditional Chinese medicine composition, and thickener; S3. Add the dispersed oil phase raw material from step S2 to the dispersed aqueous phase raw material from step S1, add emulsifier, stir for 10 min to 35 min, homogenize for 10 min to 15 min, and complete the preparation.

[0039] It should be noted that the above technical solution ensures that the polyphenols and polysaccharides in the plant fermentation extracts in the aqueous phase are not destroyed; it also promotes the physical cross-linking of decarboxylated carnosine in the oil phase with the triterpenoids and flavonoids in the traditional Chinese medicine composition. Simultaneously, the thickener fully swells at high temperature to form a three-dimensional network structure. After mixing the two phases, an emulsifier is added to reduce interfacial tension through shearing, and homogenization further reduces the droplet size to the micrometer level, forming a stable water-in-oil or oil-in-water emulsion. This ensures that the bioactivity of the traditional Chinese medicine extracts and fermentation products is uniformly distributed and remains stable over a long period within the system.

[0040] The present invention also proposes a skin care cream that uses the above-mentioned skin care composition.

[0041] The present invention will be further illustrated below through specific embodiments: All raw materials used in the embodiments of this invention are commercially available, and this invention does not impose any restrictions on the source of raw materials.

[0042] Example 1 The skin care composition in Example 1 comprises the following ingredients by weight: Decarboxylated carnosine: 110g; Plant fermentation extract: 200g; Traditional Chinese medicine composition: 50g; Emulsifier (lecithin): 15g; Emollient (sorbitol): 250g; Thickener (xanthan gum): 40g; Deionized water: 400g; The plant fermentation extract in Example 1 was prepared from 600g of bulbs of *Bulbus denticulata*, 130g of root of *Panax quinquefolia*, and 270g of stems and leaves of *Panax quinquefolia*.

[0043] The preparation process of the plant fermentation extract in Example 1 includes the following steps: T1. The bulbs of *Bulbus buergerianum* and the roots, stems and leaves of *Panax* species were pulverized and mixed with water at a ratio of 1:9. The mixture was then pulverized to obtain a slurry, sterilized, and subjected to ultrasonic extraction at 70°C with an ultrasonic frequency of 150 Hz for 60 min. Afterward, the mixture was centrifuged at 3000 r / min for 10 min to obtain the supernatant and the lower precipitate. The supernatant was collected to obtain the extract. T2. Collect the lower precipitate from step S1, add 500g deionized water, 150g pectin, 13g peptone, and a small amount of Aspergillus niger powder, and carry out aerobic fermentation at 30℃ for 5 days; then add a small amount of yeast powder and Lactobacillus powder and carry out anaerobic fermentation at 35℃ for 1 day; after fermentation, add the extract obtained in step T1, add citric acid to reach a concentration of 0.2g / L, react for 30h, centrifuge at 3000r / min for 20min, take the supernatant, and concentrate it 40 times at 60℃ to obtain the plant fermentation extract.

[0044] The dosage of Aspergillus niger powder is 1.5g, yeast powder is 1g, and lactobacillus powder is 1g.

[0045] The preparation process of the traditional Chinese medicine composition in Example 1 includes the following steps: 100g of Centella asiatica and 30g of Phyllanthus emblica were pulverized and passed through a 50-mesh sieve, then added to 400g of olive oil and extracted at a constant temperature of 75℃ for 12 hours to obtain the herbal extract.

[0046] The preparation method of the skin care composition in Example 1 includes the following steps: S1. Disperse the plant fermentation extract and emollient in half of the deionized water, heat to 80°C, and stir for 15 minutes to obtain the aqueous raw material; S2. Disperse the decarboxylated carnosine, the traditional Chinese medicine composition, and the thickener in the other half of the deionized water, heat to 95°C, and stir for 35 minutes to obtain the oil phase raw material; S3. Add the dispersed oil phase raw material from step S2 to the dispersed aqueous phase raw material from step S1, add emulsifier, stir for 35 minutes, homogenize for 10 minutes, and complete the preparation.

[0047] Example 2 Example 2 is based on Example 1, except that the skin care composition in Example 2 comprises the following ingredients by weight: Decarboxylated carnosine: 60g; Plant fermentation extract: 250g; Traditional Chinese medicine composition: 30g; Emulsifier: 20g; Emollient: 200g; Thickener: 60g; Deionized water: 500g.

[0048] Example 3 Example 3 is based on Example 1, except that the skin care composition in Example 3 comprises the following ingredients by weight: Decarboxylated carnosine: 100g; plant fermentation extract: 200g; traditional Chinese medicine composition: 60g; emulsifier: 20g; emollient: 200g; thickener: 60g; deionized water: 500g.

[0049] Comparative Example 1 Comparative Example 1 is based on Example 1, except that no traditional Chinese medicine composition is added to the skin care composition in Comparative Example 1.

[0050] Comparative Example 2 Comparative Example 2 is based on Example 1, except that no plant fermentation extracts are added to the skin care composition in Comparative Example 2.

[0051] Comparative Example 3 Comparative Example 3 is based on Example 1, except that the plant fermentation extract in Comparative Example 3 is not obtained through fermentation treatment, and the preparation method specifically includes the following steps: T1. The bulbs of *Bulbus buergerianum* and the roots, stems and leaves of *Panax* species were pulverized and mixed with water at a ratio of 1:9. The mixture was then pulverized to obtain a slurry, sterilized, and subjected to ultrasonic extraction at 70°C with an ultrasonic frequency of 150 Hz for 60 min. Afterward, the mixture was centrifuged at 3000 r / min for 10 min to obtain the supernatant and the lower precipitate. The supernatant was collected to obtain the extract. T2. Collect the lower precipitate and extract from step S1, add 500g deionized water, 150g pectin, and 13g peptone, add citric acid to make the concentration reach 0.2g / L, react for 30h, centrifuge at 3000r / min for 20min, take the supernatant, concentrate it 40 times at 60℃ to obtain the plant fermentation extract.

[0052] Performance testing (1) This test example is a whitening performance test experiment of the skin care compositions provided in Examples 1-3 and Comparative Examples 1-3, specifically a melanin synthesis inhibition rate evaluation test, the method of which is as follows: B16 melanocytes in logarithmic growth phase were harvested, digested with trypsin, and then pipetted into a single-cell suspension. Cells were counted and quantitatively seeded into 96-well plates. After 24 hours, the supernatant was discarded, and RPMI 1640 culture medium containing 10% of the test sample was added to each well. The cells were cultured at 37°C and 5% CO2 under constant temperature and humidity conditions. RPMI 1640 culture medium (containing water) was added to the control wells, and the cells were incubated for three days, with the culture medium changed once in between. The supernatant was discarded, and after routine trypsin digestion, the cell density was counted, and the cell pellet was obtained by centrifugation. The cells were washed twice with PBS, centrifuged, and the supernatant was discarded. A quantitative amount of 1 mol / L NaOH solution containing 10% DMSO was added, and the cells were incubated at 80°C for 50 min. The absorbance was then measured at 450 nm using a microplate reader.

[0053] (2) This test example is a test experiment on the collagen synthesis promotion performance of the skin care compositions provided in Examples 1-3 and Comparative Examples 1-3, specifically including: Detection of type I collagen mRNA levels: Logarithmic growth phase fibroblasts were seeded at a density of 1×10⁵ cells / mL in 96-well plates. The cells in the 96-well plates were divided into 22 groups, 21 of which corresponded to the anti-aging and skin repair skin care compositions provided in Examples 1-7 and Comparative Examples 1-14, respectively, and the remaining group served as a blank control group. The test samples were added to the cell wells to make the concentration of the active ingredient of the skin care composition in the wells 5 μg / mL. An equal volume of DMEM medium was added to the blank control group. The fibroblasts treated in the above 22 groups were incubated at 37°C and 5% CO₂ for 72 h and then collected. Total RNA was extracted using RNAiso Plus reagent (9109Takara), and then the mRNA was reverse transcribed into cDNA using the PrimeScriptRT kit. An equal amount of cDNA was then used to detect the mRNA level of collagen I by real-time fluorescence PCR using the Real-Time PCR Master Mix kit (ABI) and primers. The above specific operations were performed according to the kit instructions.

[0054] The measurement results are shown in Table 1.

[0055] Table 1

[0056] As shown in Table 1, compared with the blank control group, the skin care composition provided in this embodiment of the invention can significantly promote the proliferation of fibroblasts, and the cell number growth rate can even reach more than 70%. Moreover, the mRNA level of collagen I in fibroblasts treated with the anti-aging and repairing skin care composition is significantly increased, indicating that the anti-aging and repairing skin care composition provided in this invention can significantly upregulate the mRNA level of collagen I and has the function of promoting the production of type I collagen in fibroblasts.

[0057] The above description is merely an exemplary embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural transformations made using the present invention's specification under the technical concept of the present invention, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present invention.

Claims

1. A skin care composition, characterized in that, The skin care composition comprises the following ingredients in parts by weight: Decarboxylated carnosine: 5 to 15 parts; Plant fermentation extract: 8 to 25 parts; Traditional Chinese medicine composition: 3 to 6 parts; Emulsifier: 1 to 2 parts; Moisturizer: 20-30 parts; Thickener: 3 to 10 parts; Deionized water: 40-80 parts; The traditional Chinese medicine composition is an extract of Centella asiatica and Phyllanthus emblica; The plant raw materials for the plant fermentation extract include the bulbs of *Bulbus orientalis* and the roots, stems, and leaves of plants in the genus *Panax*.

2. The skin care composition according to claim 1, characterized in that, The preparation method of the traditional Chinese medicine composition includes the following steps: pulverizing Centella asiatica and Phyllanthus emblica into powder, adding them to the extraction solvent, and extracting at 50℃~80℃ for 10h~20h to obtain the traditional Chinese medicine composition; wherein, the weight ratio of Centella asiatica and Phyllanthus emblica is 1:(0.15~0.4).

3. The skin care composition as described in claim 2, characterized in that, The extraction solvent is selected from at least one of coconut oil, olive oil, sweet almond oil, grape seed oil, jojoba oil, rosehip oil, sesame oil, wheat germ oil, and camellia seed oil; And / or, the mass ratio of the powdered raw material to the extraction solvent is 1:(3~10).

4. The skin care composition as claimed in claim 1, characterized in that, The Bulbophyllum is selected from one or more of the following: Bulbophyllum rotundum, Bulbophyllum serrated, Bulbophyllum florida, Bulbophyllum aromaticum, Bulbophyllum fasciatus, Bulbophyllum longiflorum, and Bulbophyllum ruby. And / or, the ginseng plants are selected from one or more of ginseng, American ginseng, Panax notoginseng, Panax japonicus, and Panax quinquefolius.

5. The skin care composition as claimed in claim 1, characterized in that, The preparation process of the plant fermentation extract includes the following steps: T1. After crushing the bulbs of *Bulbus buergerianum* and the roots, stems and leaves of *Panax ginseng* plants, mix them with water at a ratio of 1: (8~15), crush to obtain a slurry, sterilize, extract by ultrasonication, centrifuge to obtain the supernatant and the lower precipitate, and collect the supernatant to obtain the extract. T2. Collect the lower precipitate from step S1, add Aspergillus niger powder, carbon source, nitrogen source and deionized water, and carry out primary fermentation; then add yeast and lactobacillus for secondary fermentation. After fermentation, add the extract obtained in step T1, add organic acid solution, react, centrifuge, concentrate, and obtain plant fermentation extract.

6. The skin care composition as claimed in claim 5, characterized in that, In step T1, the mass percentage of the bulb of *Bulbus bulgaricus* in the plant raw material of the plant fermentation extract is 55wt%~75wt%; and the mass percentage of the root of *Panax* in the plant raw material of the plant fermentation extract is 8wt%~14wt%.

7. The skin care composition as claimed in claim 5, characterized in that, In step T2, the nitrogen source is peptone and the carbon source is pectin; And / or, in step T2, the weight ratio of the lower precipitate, carbon source, nitrogen source and deionized water is (10~15):(2~4):(0.5~0.9):(14~30). And / or, in step T2, the organic acid solution is selected from at least one of citric acid, lactic acid, formic acid, acetic acid, malic acid, and tartaric acid; And / or, in step S4, the amount of organic acid solution used is 0.05 g / L to 0.6 g / L, the reaction time is 24 h to 72 h, and the concentration ratio is 40 to 60 times.

8. The skin care composition as claimed in claim 5, characterized in that, In step T2, during the first fermentation, aerobic fermentation is carried out at a temperature of 28℃~36℃ and a fermentation time of 4d~8d. And / or, in step T2, during the secondary fermentation, anaerobic fermentation is carried out, the pH value is adjusted to 4.5~5.5, the fermentation temperature is 25~35℃, and the time is 1d~2d.

9. A method for preparing a skin care composition as described in any one of claims 1 to 8, characterized in that, The method for preparing the skin care composition includes the following steps: S1. Disperse the aqueous raw material in half of the deionized water, stir and heat to 60℃~80℃, and keep warm and stir for 10min~25min; the aqueous raw material includes: plant fermentation extract and emollient; S2. Disperse the oil phase raw material in the other half of the deionized water, stir and heat to 90℃~110℃, and keep warm and stir for 20min~35min; the oil phase raw material includes: decarboxylated carnosine, traditional Chinese medicine composition, and thickener; S3. Add the dispersed oil phase raw material from step S2 to the dispersed aqueous phase raw material from step S1, add emulsifier, stir for 10 min to 35 min, homogenize for 10 min to 15 min, and complete the preparation.

10. A skin cream, characterized in that, The skin cream uses the skin care composition according to any one of claims 1 to 8.

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