30 results about "Cymbidium faberi" patented technology

The invention relates to the field of culturing tissues of plants, in particular to a formula of a culture medium and a method for culturing the tissues of Chinese cymbidium faberi rolfe. In the culture medium of the invention, Hyponex No.1 is taken as a basic culture medium, and 0.5 mg / L lebutyric acid, 0.2 mg / L naphthylacetic acid, 30 g / L sucrose, 4 g / L agar, 1 g / L activated carbon and 10 volume percent coconut milk are added into the basic culture medium, wherein a pH value is 5.6. In tissue culture, a selected healthy protocorm or root stock is inoculated to the culture medium and is cultured to be a seedling. The method has the advantages of simple operation, one-step seedling culture, good practical effect and capacity of reducing medicament cost, labor cost and pollution ratio and shortening the period of producing and breeding seedlings of the Chinese cymbidium faberi rolfe.

The invention relates to a three-dimensional cultivation method for culturing flowers above and fish below in a greenhouse, belongs to the field of flower culture and aquaculture and particularly relates to a three-dimensional cultivation method for cymbidium and tilapia. By controlling various conditions in the three-dimensional greenhouse, the cymbidium and the tilapia can grow simultaneously and assist each other in the northern environment. A mode of culturing the flowers above and the fish below is achieved, the aim of yielding of both the flowers and the fish is achieved, the yield per unit in the greenhouse can be effectively improved, use of pesticides is greatly reduced or even avoided on the conditions of flower and fish feeding, and accordingly the three-dimensional cultivation method improves economic benefit and has the advantages of saving energy, land and labor and being high in benefit.

The invention relates to an industrial production method of Cymbidium germchit based on sexual hybridization. The method provided by the invention comprises the following steps of: firstly identifying chromosome number and ploidy of Cymbidium parents; selecting pairing hybridized combinations according to the ploidy; carrying out a sexual hybridization pollination of the Cymbidium; managing the pollinated Cymbidium; carrying out a nonsymbiotic germination culture on Cymbidium seeds; inducing and differentiating protocorms (PLBs) of the Cymbidium seeds; differentiating bigger seedlings of the Cymbidium; transplanting the Cymbidium seedlings into bottles; and managing the cultivation of the Cymbidium transplanted seedlings. On the basis of indentifying the chromosomes of the Cymbidium parents, the method provided by the invention selectively pairs the hybridized combinations and carries out the sexual hybridization pollination, so that the aims of improving varieties and breeding new varieties are achieved; then a lot of Cymbidium germchit can be industrially produced through a tissue culture method.

The invention relates to a seed selection method for drought and heat resistant cymbidium hybridum. The method comprises the following steps: (1) carrying out the screening and parent selection on seed sources according to the standard of drought and heat resistance; (2) hybridizing the parents which have the drought and heat resistant characteristic and are selected in the step (1); (3) carrying out the seed asepsis culture; (4) carrying out the filial generation culture; and (5) carrying out the breed screening of the filial generation according to the standard of drought and heat resistance so as to complete the seed selection. The seed selection method for drought and heat resistant cymbidium hybridum can make full use of cymbidium hybridum resources at both home and abroad to culture a new cymbidium hybridum species with the proprietary intellectual property rights. The new cymbidium hybridum has the advantages of draught resistance, high temperature resistance, and adaptation to be implanted in North China.

The invention discloses a method for culturing and rapidly propagating large-flowered cymbidium. According to the invention, crossbreeding and fruit culture are carried out on orchid plants such as cymbidium lowianum, cymbidium eburneum, cymbidium wenshanense, cymbidium hybridium and the like in a resource garden; when the fruits of the cymbidiums are cultured to 90% mature and the fruit peels slightly become yellow, the fruits are picked for crossbreeding; explant treatment and seed sterile germination culture are carried out to ensure that the seeds germinate to root; transiting the root into a modified culture medium for culture; after 4-6 months, the seeds gradually become test-tube plantlets which have root systems and can be exercised and transplanted; through multiplication, induction and rooting culture, the test-tube plantlets can be taken out for exercise when the roots are about 1cm long; and after 1-2 months, culture is carried out in a greenhouse by using facilities. The method provided by the invention is not only capable of culturing new species, but also capable of carrying out rapid propagation, so as to satisfy the industrialized demands of fragrant flowers and bright colors.

The invention discloses a Cymbidium faberi rapid breeding seedling growing method. The Cymbidium faberi rapid breeding seedling growing method comprises following steps: Cymbidium faberi seeds are subjected to disinfection, are processed with gibberellin GA3, and are sowed in an aseptic germination culture medium for seedling growing at aseptic conditions, when 2 to 3 true leaves are obtained androots are complete, transplanting to a transplanting culture medium is carried out for transplanting culture, 2 to 3 months later, domestication is carried out, and at last transplanting into a transplanting medium is carried out for growth. The Cymbidium faberi rapid breeding seedling growing method is simple and convenient; cost is low; seed germination rate is as high as 30 to 50%; germinationperiod is short; transplanting survival rate is as high as 90% or higher; rapid large amount breeding of Cymbidium faberi seedlings is realized, and requirements of large area large scale seedling growing are satisfied.

The invention discloses a special culture medium for cymbidium qiubeiense. The special culture medium is prepared from the following substances in parts by weight: 70-80 parts of White culture medium, 15-20 parts of ferric sulfate, 8-10 parts of EDTA disodium, 6-8 parts of cane sugar, 6-8 parts of glucose, 8-10 parts of lactoalbumin hydrolysate, 8-12 parts of maize endosperm, 6-12 parts of tomato juice, 3-12 parts of coconut milk, 3-5 parts of heteroauxin, 2-5 parts of naphthylacetic acid, 3-6 parts of naphthoxyacetic acid, 1-5 parts of ABT rooting powder, 1-4 parts of zeatin, 1-4 parts of kinetin, 1-3 parts of isoamyl amino purine, 1-3 parts of gibberellin, 20-30 parts of agar, 5-15 parts of active carbon and 40-50 parts of water. The special culture medium for cymbidium qiubeiense disclosed by the invention is abundant in nutrition, and is prepared aiming at the plant characteristics of the cymbidium qiubeiense; the survival rate of the special culture medium can be effectively improved; and the market prospect is good.

The invention provides a preservative for potted flower of cymbidium faberi. It is characterized in that the preservative is essentially made from silver thiosulfate (STS). The preservative can maintain the quality of potted flower, make color more bright, and prolong the life length for various cymbidium faberi.

The invention relates to the technical field of planting materials of orchids, and specifically relates to a planting material for improving the growth of orchids. The formula of the planting materialincludes the following components: in parts by mass, 30-45 parts of bark, 30-40 parts of peanut shells, 2-5 parts of orchid cultivating stone, 3-8 parts of plant ash, 8-15 parts of vesuvianite, 3-8 parts of vermiculite, 3-8 parts of Quercus spinosa David ex Fr. Leaves and 5-10 parts of potassium humate. The invention also discloses a preparation method of the planting material for promotion of the growth of orchids. Through optimization of the formula and the preparation method, good promotion effects on stages of the orchid growth are achieved through the prepared planting material, good effects of water permeability and air permeability are achieved, and the fertilizer effect can last for a long period of time; the prepared planting material has good application effects on different types of orchids, and the application effects on Cymbidium goeringii, Cymbidium faberi, Cymbidium ensifolium, Cymbidium sinense and Cymbidium kanran are more significant; and meanwhile, the adopted raw materials of the planting material is simple, and fast seedling emergence, small dosage of the planting material and cost saving are achieved during growth of the orchids.

The invention discloses a special culture medium for cymbidium faberi. The special culture medium is prepared from the following substances in parts by weight: 70-80 parts of an N6 culture medium, 15-20 parts of ferric sulfate, 8-10 parts of EDTA disodium, 6-8 parts of sugarcane, 8-10 parts of lactoalbumin hydrolysate, 8-12 parts of corn endosperm, 6-12 parts of tomato juice, 3-12 parts of coconut milk, 3-5 parts of indoleacetic acid, 3-6 parts of indolebutyric acid, 2-5 parts of naphthylacetic acid, 3-6 parts of naphthoxyacetic acid, 1-5 parts of ABT rooting powder, 1-4 parts of zeatin, 1-4 parts of kinetin, 1-3 parts of isopentenylamino purine, 1-3 parts of gibberellin, 20-30 parts of agar, 5-15 parts of activated carbon and 40-50 parts of water. The special culture medium for the cymbidium faberi is prepared according to the plant characteristics of the cymbidium faberi, is rich in nutrition, is capable of effectively improving the survival rate of the cymbidium faberi and has excellent market prospect.

The invention provides a tissue culture rapid propagation method of cymbidium faberi, and belongs to the technical field of plant tissue culture. The method comprises the steps of disinfecting of pedicel bud explants, inducing culture, subculture, strong seedling culture and the like, wherein, the explants are disinfected through a method of matching alcohol disinfection and plant disinfection solution. Active ingredients of the plant disinfection solution are glycyrrhizic acid, pomegranate peel extract and herba houttuyniae extract; the media used in each culture stage are added with okra juice and folium mori juice as nutrients. The method of the invention has little harm to the pedicel bud explants, and a medium formula provided by the invention can significantly improve induction differentiation rate of the cymbidium faberi, proliferation coefficient and rooting rate of the explants, shorten cultivation period, and improve quality of cymbidium faberi tissue cultured seedlings.

The invention discloses a high-efficient seedling cultivation method for hybrid cymbidium of longibracteatum and cymbidium hybridum, which comprises the following steps: selecting new buds with strong growth vigor from the hybrid cymbidium, and treating to obtain sterilized small lateral buds and sterilized stem tips; performing induction culture on the sterilized stem tips on a protocorm induction culture medium, firstly inoculating the sterilized small lateral buds into a growth culture medium for re-greening growth culture, and then inoculating the small lateral buds into the protocorm induction culture medium for induction culture; dividing the obtained protocorm, and inoculating the protocorm into a subculture multiplication / differentiation culture medium for culturing; and cutting off the adventitious buds obtained by culturing, inoculating the adventitious buds into a rooting and seedling strengthening culture medium as buds, and culturing to obtain transplantable seedlings. The high-efficiency seedling cultivation method for hybrid cymbidium of longibracteatum and cymbidium hybridum provided by the invention is suitable for tissue culture and rapid propagation of hybrid cymbidium varieties formed by taking the longibracteatum and the cymbidium hybridum as parents, and the problem that the traditional division propagation process is slow is solved.

The invention discloses an understory cultivation method of Cymbidium, specifically (1) selecting the understory land, building a sunshade net, and connecting it to a water spray system; (2) preparing a cultivation substrate; (3) selecting and transplanting seedlings and carrying out Pretreatment; (4) transplant the pretreated transplanted seedlings and pour enough water; (5) water with a sprinkler system when the water is insufficient; (6) remove the sunshade net in winter, and lay dead branches on the substrate fallen leaves. The understory cultivation method of the cymbidium of the invention does not need to build the environment required for the growth of the cymbidium, which saves costs, and at the same time, the large-scale cultivation of the cymbidium enables the application of the cymbidium to be expanded to outdoor decorative plants.

The invention discloses a method for promoting differentiation of orchid flower buds. The method comprises the following steps of spraying a plant growth regulator to orchid leaves for the first time, then spraying the plant growth regulator for the second time every 5-20 days, watering the cymbidium for the second time on the second day after spraying the plant growth regulator for the second time, watering the cymbidium for the second time after watering the cymbidium for the first time after 10-15 days, and taking a prevention measure for preventing rainwater from watering during the period without watering the cymbidium until the cymbidium is dry, the watering follow the principle of completely watering, the completely watering should be twice in a row, after the second time of spraying, the temperature at night is 7-15 DEG C, and flower bud differentiation is promoted. According to the method, flower bud differentiation of cymbidium hybridum can be promoted, more flower buds and spikes are formed, flowering of the second year is guaranteed in regions with high temperature, high humidity and unobvious day and night temperature difference, and the method is easy and convenient to operate and beneficial to popularization and implementation.

The invention discloses a cymbidium faberi germplasm resource in-vitro preservation method and a growth recovery method after preservation. According to the invention, shoot tip is stripped from newly grown pseudobulb of cymbidium faberi, is placed in the surface of a protocorm-induced medium for culturing the protocorm, protocorm is repeatedly cut and subcultured, so that the protocorm with the required number is cultured, then an in-vitro preservation medium is employed for culture, after the protocorm is at a growth phase, is subjected to dark culture under the temperature of 0-5 DEG C; the protocorm during dark culture is transferred to a differentiation medium, and the seedling is generated by protocorm differentiation. A Cymbidium faberi stem tip culture technology is used, the protocorm is induced, and then the Cymbidium faberi seedling can be obtained through the protocorm differentiation. In the invention, the protocorm can be preserved at low temperature, a proper amount of paclobutrazol is added, so that the preservation time is greatly prolonged, subculture frequency is reduced, and the heredity characteristic of the germplasm resource is effectively kept. After preservation for certain time, the cymbidium faberi germplasm is placed under normal temperature and illumination condition for culture, growth can be rapidly recovered, and the seedling can be differentiated.

The invention discloses a cymbidium hybridum culture medium. The culture medium taking peat as a main material is poor in adsorbability and extremely likely to cause loss of nutrient substances, and soil hardening is extremely easy to cause in long-time use, so that normal growth of cymbidium hybridum is seriously influenced. The cymbidium hybridum culture medium is prepared from the following raw materials in parts by weight: 100 to 200 parts of peat, 10 to 20 parts of bark, 40 to 60 parts of mushroom dreg, 20 to 60 parts of wormcast, 1 to 2 parts of 2-acrylamide-2-methylpropanesulfonic acid, 4 to 10 parts of hyperbranched polyamide-amine and 0.2 to 4 parts of ceric ammonium nitrate. The invention further discloses a preparation method of the cymbidium hybridum culture medium. The cymbidium hybridum culture medium has unique physicochemical properties such as rich pores, large specific surface area, strong adsorbability and stability, the soft and loose structure state of the medium can be maintained according to the growth characteristics of cymbidium hybridum, hardening of the medium is avoided, and the water-retaining property and air permeability of the medium are enhanced.

The invention discloses a method for aseptic germination of Cymbidium seeds. In the method, the Cymbidium seeds are sterilized with gibberellin GA 3 Treatment, and then seeded in sterile germination medium under sterile conditions for cultivation. The method of the invention is simple and easy, low in cost, high in germination rate of 30%-50%, short in germination period, capable of quickly and massively obtaining Cymbidium seedlings, and can meet the needs of large-scale and large-scale seedling cultivation.

The invention provides a cultivation method for promoting early flowering of cymbidium hybridum seedlings, which comprises the following steps: (1) in spring of the first year, seedlings which are subjected to tissue culture and taken out of a bottle are transplanted into a first matrix of a nutrition pot, culturing is performed for 6-7 months, and Peters Professional which is diluted by 1000 times and a controlled release fertilizer which is 3-5 g / pot are applied during the period; (2) in autumn of the first year, the pot is changed, the seedlings are taken into a second substrate of a new flowerpot for culturing, Peters Professional which is diluted by 1000 times and a controlled release fertilizer which is 8-12 g / pot are applied during the period, the period is not overlapped with the fertilization time, humic acid is sprayed by 1-2 times, side buds are removed in time, and the operation is repeated until February to March of the next year; and (3) in the spring of the second year, when the second-generation buds grow to 15-20 cm, the second-generation buds continue to grow to July and August, topdressing is performed with a high phosphate fertilizer diluted by 1000 times once a week to promote flower bud differentiation and enable the flowers to be seen by naked eyes preferably, then a proper amount of compound fertilizer is applied once a week to promote growth of the flower roots, the growth period is only 2.5 years, one year is reduced compared with the prior art, and the cost is lowered by 40%.

The invention relates to the field of culturing tissues of plants, in particular to a formula of a culture medium and a method for culturing the tissues of Chinese cymbidium faberi rolfe. In the culture medium of the invention, Hyponex No.1 is taken as a basic culture medium, and 0.5 mg / L lebutyric acid, 0.2 mg / L naphthylacetic acid, 30 g / L sucrose, 4 g / L agar, 1 g / L activated carbon and 10 volume percent coconut milk are added into the basic culture medium, wherein a pH value is 5.6. In tissue culture, a selected healthy protocorm or root stock is inoculated to the culture medium and is cultured to be a seedling. The method has the advantages of simple operation, one-step seedling culture, good practical effect and capacity of reducing medicament cost, labor cost and pollution ratio and shortening the period of producing and breeding seedlings of the Chinese cymbidium faberi rolfe.

The invention provides a method for in vitro intermediate propagation of cymbidium grandiflorium. The cymbidium grandiflorium is propagated through a method of plant tissue cultivation, the influenceof external conditions can be avoided, production can be conducted in all seasons, the occupied area of seedlings can be saved, and the costs of enterprises are lowered. According to the method for invitro intermediate propagation of the cymbidium grandiflorium, at different stages, through control of a series of artificial conditions such as provision of different culture media, all good characters of a parent body are preserved, and the inherited characters are stable; and through induction differentiation, adventitious buds are obtained efficiently, test-tube plantlets formed by rooting are treated through acclimatization, the growth cycle can be shortened, the survival rate can be effectively increased, and the method for in vitro intermediate propagation of the cymbidium grandiflorium is suitable for large-scale industrial production.

The invention provides a method for shortening the flower age of cymbidium hybridum and improving the flowering rate, and relates to the field of flower cultivation, the method comprises the steps of seedling transplanting, management after transplanting, management after repotting, three-generation bud cultivation, annual flowering management and the like, auxin and chlormequat chloride are alternately sprayed on the cymbidium hybridum after repotting, and the flowering rate of the cymbidium hybridum is increased. The plant growth vigor can be suppressed while plant growth is stimulated, sufficient nutrition is provided for flower development, the proportion of flowers and leaves can be adjusted, and a better ornamental effect is achieved. In the whole growth process, the fertilizer is applied less and many times, and the growth environment elements are strictly controlled, so that the cymbidium hybridum can end the juvenile growth as soon as possible and is transited to reproductive growth, the flower age of the cymbidium hybridum is shortened to 2.5 years, and the flower age is shortened by one year compared with that of a blank control group.

The invention discloses an under-forest cultivation method for Cymbidium faberi. The method specifically comprises the following steps: (1) selecting under-forest land, building a sunshade net and connecting the land with a water spraying system; (2) preparing a culture substrate; (3) selecting transplanted seedlings and performing pretreatment; (4) transplanting the pretreated transplanted seedlings and pouring sufficient water; (5) when the water is insufficient, performing irrigation with the water spraying system; (6) removing the sunshade net in winter and laying dry branches and fallen leaves on the substrate. According to the under-forest cultivation method for Cymbidium faberi, an environment required for growth of the Cymbidium faberi does not need to be built, so that the cost issaved; the application of the cymbidium faberi can be expanded to outdoor decorative plants by cultivating the cymbidium faberi in a large range.

The invention discloses a proliferation culture medium for Jinbian cymbidium faberi. The formula of the proliferation culture medium comprises the following composition components of a 1 / 2 MS culturemedium, sugar of 20-40g / L, coconut juice of 100-120mL / L, NAA of 5-12mg / L, 6-BA of 15-20mg / L, agar of 5-10g / L and a nutrient solution of 10-20mL / L. In the process of preparing the proliferation culturemedium, the nutrient solution is prepared. The nutrient solution can open a DNA-histone compound body in plant cells or open a DNA double helix structure, DNA and RNA polymerase can be combined, transcription is performed, further a new protein can be synthesized through translation, and growth of plant cells is promoted. The nutrient solution is used, so that the proliferation efficiency of theJinbian cymbidium faberi is high. Compared with a conventional heteroauxing culture medium, for the prepared culture medium disclosed by the invention, the number of induced buds of the Jinbian cymbidium faberi can be increased, the number of plants cultured within the same culturing time is larger, and growth time required by the Jinbian cymbidium faberi is shorter. The quality of the Jinbian cymbidium faberi after being subjected to proliferation can be high.

The invention provides a nutrition liquid of cymbidium hyridus, which is composed of N, P, K, Ca, Mg, Fe, B, Ma, Zn, Cu and Mo and etc with a proper proportion. The nutrition liquid can make the cymbidium hyridus grow faster and stronger and reduce the plant diseases and insect pests.

The invention discloses a special culture medium for Cymbidium, which is prepared from the following materials in parts by weight: 70-80 parts of N6 medium, 15-20 parts of iron sulfate, 8-10 parts of disodium EDTA, 6-8 parts of sucrose, 8-10 parts of hydrolyzed milk protein, 8-12 parts of corn endosperm, 6-12 parts of tomato juice, 3-12 parts of coconut juice, 3-5 parts of indole acetic acid, 3-6 parts of indole butyric acid, 2-naphthalene acetic acid 5 parts, 3-6 parts of naphthyloxyacetic acid, 1-5 parts of ABT rooting powder, 1-4 parts of zeatin, 1-4 parts of kinetin, 1-3 parts of isopentenylaminopurine, 1-3 parts of gibberellin , 20-30 parts of agar, 5-15 parts of activated carbon, and 40-50 parts of water. The beneficial effects of the invention are: the special culture medium for Cymbidium provided by the invention is rich in nutrition, prepared according to the plant characteristics of Cymbidium, can effectively improve the survival rate of Cymbidium, and has a good market prospect.

The invention discloses a composition for controlling phytophthora root rot of cymbidium faberi rolfe. The active pharmaceutical ingredients of the composition consist of metalaxyl-M, cyazofamid and ally isothiocyanate according to the weight ratio of 1:(5-10):(0.1-0.5). The composition has a favorable control effect on phytophthora root rot of cymbidium faberi rolfe.

The invention discloses a rooting medium for Cymbidium hybridum. The rooting medium is prepared from the components: a 2 / 3 MS medium, 25-30g / L of sugar, 200g / L of mashed potatoes, 10-15 mg / L of NAA, 5-10mg / L of IBA, 2g / L of activated carbon, and 5-10g / L of agar. The rooting medium is higher in cultivating rooting rate on Cymbidium hybridum and longer in root length, and the plant growth status isbetter, so that the quality of Cymbidium hybridum is higher; and a culture solution preparation device is used in the process of preparing the rooting medium, the device enables the assembly of a nutrient solution completely without manual operation, staff burns caused by the nutrient solution overheating is avoided, after adding of the nutrient solution is completed, a carrier board can be takenout, a new carrier board can be directly placed on a support, so that the time for collecting and placing conical flasks is reduced, and the working efficiency of the culture solution is sped up.