Method for culturing and rapidly propagating large-flowered cymbidiums
A technology for orchids and large flowers, which is applied in the field of breeding and rapid propagation of new varieties of large-flowered orchids, and can solve the problems of hybrid breeding and rapid propagation of large-flowered orchids that have not been reported.
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Embodiment 1
[0029] Example 1: Breeding and propagation of BCL-1 (Bichunlan)
[0030] 1. Source of material: two parent orchid strains Jasperopsis ( Cmbidium lowianum) and exclusive spring ( C. eburneum ) is collected from the mountainous area of Yunnan, and Jasper Magnolia and Duozhanchun are the parents of each other.
[0031] 2. Training method
[0032] (1) Hybrid breeding
[0033] ① Establish an orchid resource garden. The environment in the resource garden can be manually regulated to create the best environment for the growth and development of orchids.
[0034] ② The natural flowering periods of these orchids are close to the same, and the breeding operation will be carried out when 1 / 2 of the inflorescence buds of the parents are open.
[0035] ③ Breeding operation: Clean your hands and tweezers, gently remove the parent herb cap (anther top cap) with your hands, use tweezers to pick off the pollen block of the male parent, and put it into the cavity (stigma) of the female ...
Embodiment 2
[0046] Embodiment 2: the breeding and propagation of BHL-1 (bicymbidium)
[0047] Female parent: Jasper ( C. lowianum ) ; Male parent: Cymbidium ( C. hyridus )
[0048] (1) Hybrid breeding is the same as in Example 1.
[0049] (2) Aseptic germination of seeds
[0050] ① Seed germination medium: The improved MS (i.e. 1 / 2MS) is selected as a more suitable basic medium, and the formula with growth regulator is: improved MS+6-BA2.0mg.L -1 +NAA0.5mg.L -1 . Culture conditions: the same as in Example 1.
[0051] ②After the seeds germinate, sprouts first, and then radicles grow, then transfer to the improved MS+6-BA0.3mg.L -1 +NAA0.5mg.L -1 Cultured in the culture medium, after 4 to 6 months, it gradually becomes a test-tube plantlet with a root system and can be hardened and transplanted.
[0052] (3) Proliferation, differentiation induction and rooting culture
[0053] ① Protocorm Proliferation Medium: Improved MS + 6-BA 2.0mg.L -1 + NAA 0.5mg.L -1 , cultivated for ...
Embodiment 3
[0058] Example 3: Breeding and Propagation of WHL-1 (Wilden Tiger Orchid)
[0059] Female parent: Wenshan Hongzhulan ( C. wenshanense ); male parent: Tiger Xuelan ( C. tracyanum var huanghua× C. mastersii ), hybrid breeding and seed aseptic germination are the same as in Example 1 or Example 2.
[0060] Proliferation, differentiation induction and rooting culture:
[0061] ① Protocorm Proliferation Medium: Improved MS + 6-BA3.0mg.L -1 + NAA 0.8mg.L -1 , cultivated for 42 days.
[0062] ② Protocorm differentiation into test tube seedling medium: improved MS + 6-BA2.5mg.L -1 + NAA 0.8mg.L -1 + Banana puree 80g / L, cultivated for 53 days.
[0063] ③ Rooting medium: improved MS + 6-BA0.8mg.L -1 + NAA 2.5mg.L -1 , cultivated for 36 days.
[0064] Proliferation, differentiation induction and rooting culture conditions were the same as in Example 1.
[0065]
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