Cymbidium faberi germplasm resource in-vitro preservation method and growth recovery method after preservation

An in vitro preservation and germplasm technology, applied in the field of plant tissue culture, can solve the problems of large manpower and material resources, cross-contamination, genetic variation, etc., and achieve the effects of prolonged storage time, strong regeneration ability, and stable genetic genes

Inactive Publication Date: 2017-08-15
SHANDONG CROP GERMPLASM CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Frequent in vitro subculture preservation technology may cause cross-contamination during each subcultu

Method used

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  • Cymbidium faberi germplasm resource in-vitro preservation method and growth recovery method after preservation
  • Cymbidium faberi germplasm resource in-vitro preservation method and growth recovery method after preservation

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A method for in vitro preservation of Cymbidium germplasm resources and recovery of growth after preservation, the specific steps are as follows:

[0027] (1) Peel off the shoot tip from the newly grown pseudobulb of Cymbidium, place it on the surface of the protocorm induction medium supplemented with 0.5mg / L 6-benzyl adenine in VW medium, and cultivate it into a protocorm.

[0028] Among them, the stripping method of the stem tip is as follows: select a pseudobulb with a length of 5 cm, wash the dust on the surface with tap water, peel off the outermost leaf, scrub it with alcohol with a volume concentration of 75% for 3 seconds, and put it in a concentration of 0.1 % mercuric chloride solution for 20 minutes, rinsed with sterile water for 3 times, dried in the air or blotted the surface moisture with filter paper, and stripped the shoot tips with a size of about 0.5 mm under a microscope.

[0029] Place the shoot tip on the surface of the protocorm induction medium i...

Embodiment 2

[0043] A method for in vitro preservation of Cymbidium germplasm resources and recovery of growth after preservation, the specific steps are as follows:

[0044] (1) Peel off the shoot tip from the newly grown pseudobulb of Cymbidium, place it on the surface of the protocorm induction medium supplemented with VW medium supplemented with 1 mg / L 6-benzyl adenine, and cultivate it into a protocorm.

[0045] Among them, the stripping method of the stem tip is as follows: select a pseudobulb with a length of 7 cm, clean the surface dust with tap water, peel off the two outermost leaves, scrub with alcohol with a volume concentration of 75% for 4 seconds, and put in a mass concentration of 0.1 % mercuric chloride solution for 20 minutes, rinsed with sterile water for 4 times, dried in the air or blotted the surface moisture with filter paper, and stripped the shoot tips with a size of about 0.5 mm under a microscope.

[0046] Place the shoot tip on the surface of the protocorm induc...

Embodiment 3

[0058] A method for in vitro preservation of Cymbidium germplasm resources and recovery of growth after preservation, the specific steps are as follows:

[0059] (1) The shoot tip was stripped from the newly grown pseudobulb of Cymbidium, and placed on the surface of the protocorm induction medium supplemented with VW medium supplemented with 0.8mg / L 6-benzyl adenine to cultivate protocorms.

[0060] Among them, the stripping method of the stem tip is as follows: select a pseudobulb with a length of 6 cm, clean the surface dust with tap water, peel off one leaf of the outermost layer, scrub it with alcohol with a volume concentration of 75% for 4 seconds, and put it in a mass concentration of 0.1 % mercuric chloride solution for 20 minutes, rinsed with sterile water for 3 times, dried in the air or blotted the surface moisture with filter paper, and stripped the shoot tips with a size of about 0.5 mm under a microscope.

[0061] Place the shoot tip on the surface of the protoc...

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Abstract

The invention discloses a cymbidium faberi germplasm resource in-vitro preservation method and a growth recovery method after preservation. According to the invention, shoot tip is stripped from newly grown pseudobulb of cymbidium faberi, is placed in the surface of a protocorm-induced medium for culturing the protocorm, protocorm is repeatedly cut and subcultured, so that the protocorm with the required number is cultured, then an in-vitro preservation medium is employed for culture, after the protocorm is at a growth phase, is subjected to dark culture under the temperature of 0-5 DEG C; the protocorm during dark culture is transferred to a differentiation medium, and the seedling is generated by protocorm differentiation. A Cymbidium faberi stem tip culture technology is used, the protocorm is induced, and then the Cymbidium faberi seedling can be obtained through the protocorm differentiation. In the invention, the protocorm can be preserved at low temperature, a proper amount of paclobutrazol is added, so that the preservation time is greatly prolonged, subculture frequency is reduced, and the heredity characteristic of the germplasm resource is effectively kept. After preservation for certain time, the cymbidium faberi germplasm is placed under normal temperature and illumination condition for culture, growth can be rapidly recovered, and the seedling can be differentiated.

Description

technical field [0001] The invention relates to a method for in vitro preservation of Cymbidium germplasm resources and recovery of growth after preservation. The invention belongs to the technical field of plant tissue culture. Background technique [0002] Cymbidium faberi Rolfe (Cymbidium faberi Rolfe) is a ground herb of the genus Orchidaceae, with inconspicuous pseudobulbs, 5-8 leaves, belt-shaped, strong uprightness, the base is often folded in half to form a V shape, the veins are translucent, and the edges are often thick. jagged. Cymbidium is very famous among Cymbidium varieties. It is native to China and is a rare species in China. It is also a national second-class protected wild species. It is one of the longest and most popular Cymbidiums cultivated in my country. The color of Cymbidium is usually yellow-green, with dark purple spots and veins. Its flowers are rich in fragrance, and there are many flowers on one stem, usually 6-15 flowers. The propagation me...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01N3/00
CPCA01H4/001A01H4/005A01H4/008A01N3/00
Inventor 颜廷进邓翠霞戴双李群田茜
Owner SHANDONG CROP GERMPLASM CENT
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