A kind of in vitro rapid propagation method of Cymbidium cymbidium

A fast, in vitro technology, applied in the field of artificial propagation and cultivation of plants, can solve problems such as inability to increase nutritious endosperm and other tissues, incomplete embryo development, and decline of orchid varieties, so as to relieve healing and infection, shorten The effect of growth cycle and space saving

Active Publication Date: 2020-06-05
HUBEI HONGXING LANDSCAPING PROJECT CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional reproduction of orchids is branch reproduction. However, a robust orchid can only reproduce a few buds a year, the reproduction coefficient is extremely low, and the multiplication multiple is only 1-3 times. Many rare varieties cannot be reproduced in large quantities. It is far from being able to meet the demand of the market, and the price has remained high for a long time, and each leaf bud is as high as tens of thousands of yuan
Many varieties cannot or seldom produce seeds under cultivation conditions. If they reproduce sexually, because the seeds of orchids are small and the embryos are not fully developed, they cannot increase the nutritious endosperm and other tissues, and it is not easy to germinate. It is possible to germinate only when specific symbiotic fungi provide nutrition; many rare varieties are actually some natural mutants. If seeds are produced through the process of sexual reproduction, and then the offspring are reproduced by seeds, then most of the individuals of this variety will lose The unique ornamental value of the original variety, in addition, the long-term asexual reproduction has caused an increasing number of plants with viruses, which has seriously reduced the variety of orchids

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of in vitro rapid propagation method of Cymbidium cymbidium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The invention provides a method for in vitro rapid propagation of Cymbidium, comprising the following steps:

[0030] S1, selection and preparation of explants: under the condition of temperature of 15°C and air humidity of 45%, young shoots with full axillary buds and not yet germinated were cut, leaves were removed, and then the shoots were cut into small sections of 1 cm. section with at least one pair of axillary buds;

[0031] S2, disinfection of explants: wash 3 times with sterilized water, blot dry with filter paper, soak in 65% alcohol for 30 seconds, soak in 0.5% sodium hypochlorite for 5 minutes, and soak in 0.5% sodium hypochlorite for 5 minutes. Soak in 0.1% mercuric chloride for 10 minutes, soak in 10% bleaching powder for 5 minutes, rinse with sterilized water for 5 times, and blot dry with filter paper;

[0032] S3, Axillary Bud Induction: Cut the sterilized Cymbidium cymbidium stem into 0.5 cm bud-bearing segments, inoculate them into medium 1, and keep...

Embodiment 2

[0038] The invention provides a method for in vitro rapid propagation of Cymbidium, comprising the following steps:

[0039] S1, selection and preparation of explants: under the condition of temperature of 22°C and air humidity of 50%, young shoots with full axillary buds and not yet germinated were cut, leaves were removed, and then the shoots were cut into small sections of 2 cm. section with at least one pair of axillary buds;

[0040] S2, explant disinfection: wash 4 times with sterilized water, blot dry with filter paper, soak in 70% alcohol by mass percentage for 45 seconds, soak in 1% sodium hypochlorite for 10 min, and soak in 1% by mass concentration of sodium hypochlorite for 10 min. Soak in mercuric chloride with a concentration of 0.3% for 12 minutes, soak in bleaching powder with a concentration of 15% by mass for 8 minutes, rinse with sterilized water for 8 times, and blot dry with filter paper;

[0041] S3, axillary bud induction: Cut the sterilized Cymbidium c...

Embodiment 3

[0047] The invention provides a method for in vitro rapid propagation of Cymbidium, comprising the following steps:

[0048] S1, selection and preparation of explants: cut shoots with full axillary buds and not yet germinated at a temperature of 25°C and an air humidity of 60%, remove the leaves, and then cut the shoots into small sections of 3 cm. section with at least one pair of axillary buds;

[0049] S2, disinfection of explants: wash 3-5 times with sterilized water, blot dry with filter paper, soak in 75% alcohol for 60 seconds, soak in 1.5% sodium hypochlorite for 15 minutes, and Soak in 0.5% mercuric chloride by mass for 15 minutes, soak in 20% by mass bleach for 10 minutes, rinse with sterilized water 10 times, and blot dry with filter paper;

[0050] S3, axillary bud induction: cut the sterilized Cymbidium cymbidium stem into 1.5 cm bud-bearing segments, and inoculate them into medium 1, at a temperature of 25°C, light intensity of 3500 lx, light time of 12 hours, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for in vitro intermediate propagation of cymbidium grandiflorium. The cymbidium grandiflorium is propagated through a method of plant tissue cultivation, the influenceof external conditions can be avoided, production can be conducted in all seasons, the occupied area of seedlings can be saved, and the costs of enterprises are lowered. According to the method for invitro intermediate propagation of the cymbidium grandiflorium, at different stages, through control of a series of artificial conditions such as provision of different culture media, all good characters of a parent body are preserved, and the inherited characters are stable; and through induction differentiation, adventitious buds are obtained efficiently, test-tube plantlets formed by rooting are treated through acclimatization, the growth cycle can be shortened, the survival rate can be effectively increased, and the method for in vitro intermediate propagation of the cymbidium grandiflorium is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of artificial propagation and cultivation methods of plants, in particular to a method for in vitro rapid propagation of Cymbidium cymbidium. Background technique [0002] With the continuous improvement of people's living standards, flowers have entered the daily life of ordinary people, and the consumption demand is also increasing. Since ancient times, orchids have been the embodiment of elegance and otherworldliness, and have high ornamental and economic value. In the international market, both cut flower varieties and potted flowers occupy an important position and are very popular among consumers. [0003] Cymbidium, with long green leaves, bold and magnificent flowers, is a world-renowned "Orchid Nova". Its plants and flowers are divided into large and medium-sized, and the flowers are huge, yellow, white, green, red, pink As well as multi-color and other colors, the colors are bright and colorful, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈丽红
Owner HUBEI HONGXING LANDSCAPING PROJECT CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap