Proliferation culture medium for Jinbian cymbidium faberi

Abstract

The invention discloses a proliferation culture medium for Jinbian cymbidium faberi. The formula of the proliferation culture medium comprises the following composition components of a 1 / 2 MS culturemedium, sugar of 20-40g / L, coconut juice of 100-120mL / L, NAA of 5-12mg / L, 6-BA of 15-20mg / L, agar of 5-10g / L and a nutrient solution of 10-20mL / L. In the process of preparing the proliferation culturemedium, the nutrient solution is prepared. The nutrient solution can open a DNA-histone compound body in plant cells or open a DNA double helix structure, DNA and RNA polymerase can be combined, transcription is performed, further a new protein can be synthesized through translation, and growth of plant cells is promoted. The nutrient solution is used, so that the proliferation efficiency of theJinbian cymbidium faberi is high. Compared with a conventional heteroauxing culture medium, for the prepared culture medium disclosed by the invention, the number of induced buds of the Jinbian cymbidium faberi can be increased, the number of plants cultured within the same culturing time is larger, and growth time required by the Jinbian cymbidium faberi is shorter. The quality of the Jinbian cymbidium faberi after being subjected to proliferation can be high.

Description

Technical field [0001] The invention belongs to the technical field of orchid culture, and specifically relates to a propagation medium for Cymbidium euphorbiae. Background technique [0002] Orchid, a traditional Chinese flower, has rich germplasm resources, is widely distributed, and has high ornamental value. Its propagation method is generally ramification propagation, which can only be carried out once 3-4 years, and the propagation speed is slow, which is difficult to meet market needs Although the orchid seeds are very small, the number is amazing. There are millions of seeds in a capsule. The number of orchid seeds is very large. However, because the orchid seeds are very small and powdery, their structure can only be seen under a microscope. There is no endosperm, only a simple embryo, with a loose, transparent, and impermeable seed coat on the outside. The embryo contains few nutrients, most of which are lipids. The orchid seeds that exist under natural conditions are o...

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Problems solved by technology

[0002] Orchid, a traditional famous flower in China, is rich in germplasm resources, widely distributed, and has high ornamental value. Its propagation method is generally branch propagation, which can only be carried out once every 3-4 years. The propagation speed is slow and it is difficult to meet the market demand. , although the orchid seeds are small, the number is amazing. There are millions of seeds in a capsule. The number of orchid seeds is very large, but because the orchid seeds are very small and powdery, their structure can only be seen clearly under a microscope. There is no endosperm, only a simple embryo, which is surrounded by a loose, transparent, impermeable seed coat. The embryo contains very little nutrients, most of which are lipid substances. The orchid seeds that exist under natural conditions can only be suitable for The fungal infection can successfully germinate, and the germination rate is very low, generally not more than 5%.

[0003] Cymbidium, also known as Jiuzilan, Jiujielan, and Xialan, is a large species of orchids in China. It is one of the oldest and most popular orchids. Because of its elegant plant type, strong fragrance, and beautiful flower shape, it is popular in Southeast Asia. Regarded as a high-end potted flower, Cymbidium grows naturally in hillside forests. Most of the cultivated varieties are domesticated from wild species or selected from natural variation. Among them, Cymbidium in Phnom Penh is rarer and has great ornamental value, so Phnom Penh The artificial proliferation of Cymbidium has gradually come into people's sight. In the process of proliferation and cultivation of Phnom Penh Cymbidium, plant proliferation medium is needed. The traditional plant proliferation medium needs to add a lot of trace elements and auxin in the preparation process. To ensure the normal proliferation of plants, the use of a large amount of trace elements and auxins increases the cost of medium preparation, and the traditional plant proliferation medium is not effective for plant proliferation. The number of induced buds and The value-added coefficients are all very low, which makes it difficult to increase the proliferation and cultivation of Phnom Penh Cymbidium, and the plant multiplication efficiency is low, and the quality of Phnom Penh Cymbidium cultivated is low

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