69results about How to "Adjust osmotic pressure" patented technology

Disclosed is a method for extracting dihydro quercetin from larch. The invention is an improved production method. Due to different production materials and extraction processes, the dihydro quercetin products in U.S. and European markets are very expensive, at a price of 200-1000 euros per gram. Such a high market price economically limits the wide range of practicability of dihydro quercetin products. During the pretreatment of raw materials, 50 kilograms of larch wood chips and 500-800 liters of 50% ethanol solution are added into a reactor to get fully stirred and mixed; then the mixture is extracted and filtered for the second extraction; the filtrates are combined and adsorbed; the organic solvents are collected and dried by a vacuum evaporator through organic phase vacuum distillation; according to a weight proportion of 10:1, the residues are dissolved with hot water, decolorized with activated carbon and then crystallized at a temperature of 3-5 DEG C to precipitate the dihydro quercetin product which is then crushed after vacuum drying to finished product. The invention is used to extract dihydro quercetin from larch.

The invention relates to a cell cryopreservation protection composition, use thereof and a cell cryopreservation method. The cell cryopreservation protection composition contains amphipathic molecules with a general formula structure of R1-N+ (CH3)2-(CH2) n-R2, wherein R1 is alkyl of a straight chain or a branch chain of the carbon numbers 1 to 10, and is optionally substituted by a substituent; R2 is selected from an optional group with negative charge in a group consisting of COO-, -SO4-, -SO3- and a compound as shown in the description; R3 is selected from a group in a group consisting of (methyl) acryoyloxyalkyl, alkyl and alkenyl; and the amphipathic molecules with the general formula structure of R1-N+ (CH3)2-(CH2) n-R2 are a betaine compound preferably. The cell cryopreservation protection composition can implement cell cryopreservation non-toxically and efficiently, and after resuscitation of cells, the yield of the cells is quite high, and gradient cryopreservation is not required; and after resuscitation of the cells, the cells can be directly used or are slightly diluted and then used.

The invention belongs to the technical field of feed for laying hens and particularly relates to complete feed for laying hens at brood time and a preparation method thereof. The complete feed comprises, by weight, 37-44% of corn, 18-22% of extruded corn, 0.4-0.6% of soybean oil, 18-20% of soybean meal, 9-11% of extruded soybeans, 1.5-2.5% of fish meal, 3.5-4.5% of fermented soybean meal, 1.1-1.2% of mountain flour, 1.4-1.7% of calcium hydrophosphate, 0.14-0.16% of methionine, 0.09-0.13% of choline chloride, 0.1-0.2% of baking soda, 0.2-0.3% of salt, 0.13-0.16% of microecologics, 0.01-0.03% of liquid phytase, 0.03-0.05% of liquid xylanase, 0.1-0.2% of acidifier, 0.1-0.2% of glycine betaine, 0.004-0.006% of antioxidant, 0.015-0.035% of 4% enramycin, 0.03-0.04% of vitamin complex and 0.08-0.09% of composite microelement. The complete feed can meet nutrition requirements of the laying hens at the brood time, improves immunity of the laying hens at the brood time, has remarkable effects on increasing weight and shin length of the laying hens at the brood time, enables the laying hens to reach the standard in advance, and provides a good foundation for achieving high yield at an egg producing period.

Disclosed is a preparation method for extracting dihydro quercetin from larch. The invention is an improved production method. Due to different production materials and extraction processes, the dihydro quercetin products in U.S. and European markets are very expensive, at a price of 200-1000 euros per gram. Such a high market price economically limits the wide range of practicability of dihydro quercetin products. During the pretreatment of raw materials, 50 kilograms of larch wood chips and 500-800 liters of 50% ethanol solution are added into an extractor to get fully stirred and mixed; then the mixture is extracted and filtered for the second extraction; the filtrates are combined and adsorbed; the organic solvents are collected and dried by a vacuum evaporator through organic phase vacuum distillation; according to a weight proportion of 10:1, the residues are dissolved with hot water, decolorized with activated carbon and then crystallized at a temperature of 3-5 DEG C to precipitate the dihydro quercetin product which is then crushed after vacuum drying to finished product. The invention is used to extract dihydro quercetin from larch.

The invention discloses a neural stem cell cryoprotectant and a using method thereof. The cryoprotectant contains a DMEM/F12 basal culture medium, dimethyl sulfoxide, serum, albumin, a B27- additive,epidermal growth factors and alkaline fibroblast growth factors. The cryoprotectant is low in cost, and solves the problem that the anabiosis survival rate after cryopreservation of neural stem cellsis low; after the cells are cryopreservated for one year by adopting the neural stem cell cryoprotectant and the using method thereof disclosed by the invention, the cell viability still reaches up to95% higher, and the attribute and differentiative capacity of the neural stem cells are not influenced.

The invention relates to an atomizing agent with arformoterol and glycopyrronium bromide serving as active components and a preparation method of the atomizing agent. The invention further relates to an application of glycopyrronium bromide in improving the stability of an arformoterol water solution. Arformoterol and glycopyrronium bromide can perform a synergistic function pharmacologically while glycopyrronium bromide is used for improving the stability of arformoterol, and accordingly, the local treatment function on the lung is improved.

The invention relates to a special-purpose formaldehyde scavenging agent for an air purifier. The special-purpose formaldehyde scavenging agent is prepared from the following components in parts by volume: 10-50 parts of kernel extracting liquor, 10-20 parts of radix angelicae extracting liquor, 5-15 parts of pomegranate bark extracting liquor, 5-20 parts of rhizoma cyperi extracting liquor, 1-5 parts of reed rhizome extracting liquor, 1-10 parts of extracting liquor of bark of white mulberry root, 0.5-5 parts of cananga odorata essential oil, 0.5-10 parts of tea tree essential oil, 1-15 parts of propylene glycol, 10-30 parts of absolute ethyl alcohol and 30-50 parts of water, wherein the kernel extracting liquor is at least one of hawthorn kernel extracting liquor, peach kernel extracting liquor and apricot kernel extracting liquor. The special-purpose formaldehyde scavenging agent does not contain substances including chlorine and the like which are harmful to a human body, contains various components including plant flavonoid, organic acid and the like which are beneficial to the human body, is strong in pertinence of scavenging the formaldehyde, quick in speed, and specially used for the air purifier, thereby strengthening immunity of a user, killing bacteria and viruses in air and improving air quality. Besides, the special-purpose formaldehyde scavenging agent has the advantages of being nontoxic, harmless, good in formaldehyde scavenging effect, free of stimulus to the human body, suitable for long-term use, incapable of causing secondary pollution, and capable of preventing bacteria and eliminating peculiar smell.

The invention discloses an Asiatic cotton protoplast separation and exogenous gene transformation method. The method comprises the steps of Asiatic cotton seed germination, enzymolysis separation of protoplast, centrifugal collection of the protoplast, culture, exogenous gene transformation, microscopic observation and identification and the like. The concentration of the protoplast which is separated from leaves growing in a dark environment meets the experimental requirements, and the integrity is high so that the intact successfully-transformed protoplast can be obtained; therefore, by means of the method for separating the protoplast from the leaves cultured in the dark environment, the purposes of Asiatic cotton protoplast separation and exogenous gene transformation are achieved better. By means of Asiatic cotton protoplast separation and successful exogenous gene transformation, basic help can be provided for carrying out large-scale cell culture of Asiatic cotton, transferringexogenous genes on purpose and carrying out further innovation and quality improvement work.

The invention provides an eye drop for repairing injured cytokines of eyes and a preparation method, and relates to the technical field of biological stem cells. Every 1 liter of eye drop comprises the following components according to a weight ratio: 10 to 15g of retinoic acid, 25 to 30g of taurine, 12 to 18g of hyaluronic acid, 2 to 5mg of benzalkonium bromide and the balance of umbilical cord mesenchymal stem cell supernatant. The eye drop has the advantages that the microenvironment of the eyes is improved; the stem cells are accelerated to migrate and differentiate to the injured part ofthe eyes, and are used as seed cells to participate in the repair and regeneration of the injured tissues of the eyes, so as to reach the purposes of repairing the tissue cells of the eyes and recovering the vision function of the eyes.

The invention discloses a cut flower fresh-keeping agent which is composed of 1 to 3 wt% of sugar, 0.1 to 0.3 mmol.L of 8-hydroxyquinoline salt and 30-120 mu mol.L of resveratrol and/or sodium nitroprusside. The invention provides a scientific basis for the application of sodium nitroprusside on the cut flower fresh-keeping; the cut flower fresh-keeping agent prepared by pure natural plant extractive resveratrol delays the ageing process of cut flowers, prolongs vase life, and effectively avoids toxic action of Ag and other injurants on the environment; and the resveratrol has wide source, and is economic and practical, natural and environmental-friendly, and safe and stable, thus having wide application prospect in the future gardening post harvest industry when being used to prepare cut flower fresh-keeping agent.

The invention discloses a feed for laying hens in the reserve period. The feed is composed of following components in parts by weight: 150 to 180 parts of MDCP, 170 to 200 parts of stone powder, 30 to 40 parts of salt, 20 to 40 parts of sodium bicarbonate, 8 to 12 parts of choline, 25 parts of compound trace elements, 5 to 6 parts of compound vitamin, 8 to 12 parts of hydroxyl methionine, 5 to 9 parts of methionine, 25 to 35 parts of lysine, 100 to 150 parts of fish meal, 300 to 330 parts of puffed soybean, 20 to 80 parts of zeolite powder, 1 to 2 parts of nosiheptide, 0.5 to 1 part of dinitolmide, 1 to 2 parts of phytase and 2 to 5 parts of betaine. The weight and neck length of the laying hens fed by the feed exceed the breeding standard in the 70th day, the weight of the laying hens arrives at the standard in the 110th day, and sexual maturity and body maturity of the laying hens are synchronous.

The invention is applicable to the field of compound fertilizers, and discloses a root-promoting and seedling-strengthening functional compound fertilizer and a preparation method thereof. The root-promoting and seedling-strengthening functional compound fertilizer comprises the following components in parts by weight: 0.01-2.5 parts of a plant growth conditioner, 88-99 parts of an inorganic nutrient and 1-5 parts of an organic nutrient. The plant growth conditioner is prepared from carboxyl polyoxyethylene phosphate potassium salt and gamma-aminobutyric acid. The preparation method is used for preparing the root-promoting and seedling-strengthening functional compound fertilizer. According to the root-promoting and seedling-strengthening functional compound fertilizer and the preparationmethod thereof, the osmotic pressure of plant cells can be adjusted, crops are stimulated to release more induced resistance factors, the saline-alkali resistance, cold resistance, high and low temperature resistance, drought resistance and the like of the crops are enhanced, meanwhile, the photosynthetic utilization rate can be increased, the crop growth is promoted, and the crop yield is increased.

The invention relates to a skin antibacterial agent, which is prepared from the following components in volume proportion: 10-50 parts of Chinese hawthorn seed extracting solution, 10-20 parts of radix angelicae extracting solution, 5-15 parts of golden cypress extracting solution, 1-5 parts of eucalyptus essential oil, 1-5 parts of tea tree essential oil, 2-10 parts of glycerine, 10-20 parts of absolute ethyl alcohol, and 30-50 parts of water. The skin antibacterial agent disclosed by the invention does not contain harmful substances to human body, such as chlorine and the like, and contains a plurality of beneficial components to human body, such as plant flavonoids, organic acid and the like; bacteria and virus on skin can be killed well; furthermore, the skin antibacterial agent is free from pollution and harmless to human-body skin and mucosa, therefore, the skin antibacterial agent is applied to being used for a long time; the tea tree essential oil and the glycerine have the effects of softening and moisturizing skin; if the skin antibacterial agent is used for a long time, skin can be improved; the skin antibacterial agent disclosed by the invention has the advantages of being nontoxic, harmless, non-irritant to human-body skin and mucosa, applied to being used for a long time, free from pollution, strong in anti-bacterial and anti-virus ability and incapable of disturbing skin growth balance.

The invention provides a different vitamin C potassium as well as a preparation method and application thereof, and relates to the technical field of chemical synthesis. The preparation method of thedifferent vitamin C potassium provided by the invention comprises the steps of: taking 2-keto-D-gluconic acid as a raw material, adding alcoholic solvents and catalysts to conduct esterification reaction, then adding basic potassium ion donors for conversion reaction. Equipment upgrading pressure is low, transformation cost is low, the operation is simple, and the practicality is strong. By-products with no economic additional value and high environmental treatment costs are not produced, cost of environmental protection is saved while economic benefits are produced, and large-scale industrialproduction can be conducted. The different vitamin C potassium provided by the invention is prepared by using the preparation method, has high purity, and can be used for supplementing potassium elements needed by a human body. The invention provides application of the different vitamin C potassium as an antioxidant and freshness preventing agent, does not bring about excessive intake of sodium and causes disease, and is safer to eat on the technology of ensuring freshness preservation.

The invention discloses a scrophularia ningpoensis planting fertilizing method for the purpose of solving the problem that in the prior art, scrophularia ningpoensis is in poor growth since only farm manure or inorganic fertilizer is used. The method comprises the first step of applying base fertilizer, wherein 22500-30000 kg/hm<2> of decomposed farm manure is applied in the soil preparation process; the second step of applying seed fertilizer, wherein after seeding and earthing, 22500-30000 kg/hm<2> of decomposed farm manure and 750 kg/hm<2> of calcium magnesium phosphate fertilizer are mixed and stirred to be uniform before being applied into positions 10 cm away from holes; the third step of applying seed bed fertilizer three times during the growing period of the scrophularia ningpoensis, wherein the first time of application is performed in the full stand period, the second time of application is performed when the scrophularia ningpoensis grows to be 15 cm high, and the third time of application is performed when the scrophularia ningpoensis grows to be 35 cm high; the fourth step of applying root promoting fertilizer, wherein at the beginning of bud appearance, 1500 kg/hm<2> of decomposed farm manure and 750 kg/hm<2> of superphosphate are mixed, stirred and piled wet, then 2000 kg/hm<2> of plant ash is added, and the mixture is applied into furrows opened beside plants. Through more reasonable matching of fertilizer and a more reasonable using amount, growth of the scrophularia ningpoensis is promoted, and output of the scrophularia ningpoensis is increased.

The invention relates to a peculiar smell removing antibacterial agent for a refrigerator. The peculiar smell removing antibacterial agent comprises the following components in parts by weight according to the following volume ratio: 10-50 parts of a peach pit extracting solution, 10-20 parts of an angelica root extracting solution, 5-15 parts of a pomegranate bark extracting solution, 1-5 parts of ginger essential oil, 1-5 parts of lemon essential oil, 1-5 parts of tea tree essential oil, 10-20 parts of absolute ethyl alcohol and 30-50 parts of water. The peculiar smell removing antibacterial agent provided by the invention does not contain chemical substances which are harmful to human bodies such as aldehydes, chlorine, peroxide and phenols, and contains the constituents which are beneficial for the human bodies including various plant flavones, organic acids, inorganic salts and amino acids, the peculiar smell removing antibacterial agent has a good effect of killing bacteria, even if being eaten by a person, the peculiar smell removing antibacterial agent is harmless but is beneficial for the human bodies; the lemon essential oil, the tea tree essential oil and the ginger essential oil can effectively remove peculiar smell in the refrigerator; the peculiar smell removing antibacterial agent has the advantages of being non-poisonous, nuisanceless and pollution-free, and strong in peculiar smell removing and sterilization properties, causing no secondary pollution, having no stimulation to human bodies, and being suitable for long-term use.

The invention relates to a tannin ointment and a preparation method thereof. The tannin ointment is prepared from tannin, glycerin, resina liquidambaris, betaine, Vaseline, tween-80, and sodium bisulfite. The preparation method of the tannin ointment comprises the following steps of dissolving the tannin by the glycerin, so as to obtain a tannin glycerin solution; adding the betaine and the sodium bisulfite, heating, and uniformly stirring, so as to obtain a component A; heating the Vaseline, the resina liquidambaris and the tween-80, and uniformly stirring, so as to obtain a component B; heating the component A and the component B to 75 to 80 DEG C, adding the component A into the component B, uniformly stirring, and cooling to room temperature, so as to obtain the tannin ointment. The tannin ointment has the advantages that the betaine has the functions of sterilizing bacteria and diminishing inflammation, adjusting cell osmosis pressure, reducing irritation of medicine to skin and the like; the resina liquidambaris has the effects of activating blood circulation and relieving pain, detoxifying, generating muscle and the like, enhances the treatment effect of tannin, and has better synergistic action with the betaine; the resina liquidambaris can be well cooperated with the bataine, so that the effect of reducing the irritation to the skin by the medicine is more obvious under the synergistic action of the resina liquidambaris and the bataine.

The invention relates to hand-washing-free antibacterial spray. The hand-washing-free antibacterial spray is prepared from the following components in parts by volume: 10-15 parts of kernel extracting liquor, 1-5 parts of tea tree essential oil, 1-5 parts of mint essential oil, 2-10 parts of glycerol, 10-20 parts of absolute ethyl alcohol and 30-50 parts of water, wherein the kernel extracting liquor is at least one of peach kernel extracting liquor and apricot kernel extracting solution. The hand-washing-free antibacterial spray does not contain substances including chlorine and the like which are harmful to a human body, contains various components including plant flavonoid, organic acid and the like which are beneficial to the human body, can be used for killing bacteria and viruses on skin very well, does not generate pollution, has no stimulus to body skin and mucosae, and is suitable for long-term use. During use, the hand-washing-free antibacterial spray is directly sprayed on the hand skin; the mint essential oil and glycerol have effects of softening skin and moistening skin, and therefore, the hand-washing-free antibacterial spray can be used for improving the skin by long-term use, and has the advantages of being nontoxic, harmless, free of stimulus to body skin and mucosae, suitable for long-term use, incapable of causing pollution, strong in bacteria-preventing and virus-killing capacity, and capable of not disturbing growth balance of the hand skin.

The invention discloses a ganoderma lucidum containing traditional Chinese medicinal composition for improving sleep. The traditional Chinese medicinal composition consists of the following componentsin parts by weight: 40-60 parts of radix ginseng, 70-90 parts of poria cocos, 70-90 parts of semen ziziphi spinosae, 50-60 parts of fruit of Chinese magnoliavine and 18-22 parts of ganoderma lucidumspore powder. The invention also provides a preparation method of the traditional Chinese medicinal composition, wherein the preparation method comprises the following steps: firstly, extracting volatile oil ingredients in the poria cocos, semen ziziphi spinosae and the fruit of Chinese magnoliavine; then, digesting and extracting the volatile oil ingredients by virtue of an ethanol water solution; and finally, mixing an obtained extract with the ganoderma lucidum spore powder. The ganoderma lucidum containing traditional Chinese medicinal composition for improving sleep provided by the invention, from the coordination in such aspects as nourishing, tranquilizing, qi calming and the like through various traditional Chinese medicinal components simultaneously, is beneficial for regulating human nerve center and regulating the osmotic pressure of human body fluid, so that effects of calming heart, soothing qi and improving sleep can be achieved. With the application of the method provided by the invention, an extraction rate of active ingredients in the various traditional Chinese medicinal components can be effectively improved, the active ingredients in the various traditional Chinese medicinal components can be preserved better and an utilization rate on pharmacological ingredients can be improved.

The invention discloses a preparation method for a phosphate buffer solution. 5 L of the concentrated phosphate buffer solution comprises potassium dihydrogen phosphate with an addition concentrationof 0.55 wt%, disodium hydrogen phosphate dihydrate with an addition concentration of 2.37-2.38 wt%, potassium chloride with an addition concentration of 1.50 wt%, sodium nitrate with an addition concentration of 0.19%-0.20 wt%, disodium edetate dihydrate with an addition concentration of 0.38-0.40 wt%, Triton X-100 with an addition concentration of 0.05-0.055 wt%, and water used as a solvent. During preparation of the phosphate buffer solution of the invention, a large amount of foam is not generated; and the method is simple to operate and greatly improved in efficiency, and can easily control the pH value and conductivity of the phosphate buffer solution.

The invention discloses electrolyte formula powder for preoperative patient nutrition supplement and a preparation method thereof. The electrolyte formula powder for preoperative patient nutrition supplement comprises the following components in parts by weight: 60-80 parts of maltodextrin, 10-30 parts of crystalline fructose, 1-20 parts of anhydrous glucose, 1-4 parts of a sodium element supplement and 1-4 parts of a potassium element supplement. The preparation method of the electrolyte formula powder for preoperative patient nutrition supplement comprises the following steps: a) performingweighing, b) performing crushing, c) performing premixing, d) performing total mixing and e) performing subpackaging. The prepared formula powder is beneficial to regulating osmotic pressure, preventing metabolic disorder, maintaining organism balance and accelerating patient recovery, and is suitable for nutrition supplement of patients of elective operation.

The invention provides an excrement exfoliated cell nucleic acid preservation reagent as well as a preparation method and an application thereof. The excrement exfoliated cell nucleic acid preservation reagent comprises a nonionic surfactant, an anionic surfactant, an osmotic pressure regulator, a cell membrane stabilizer, a chelating agent, a pH buffer solution, water and a deodorant. The preservation reagent disclosed by the invention can be used for effectively cracking human exfoliated cells in excrement, and does not contain guanidinium thiocyanate, guanidinium isothiocyanate and other toxic components, so that a method for directly extracting and detecting the excrement genome from an excrement supernatant is provided, the interference of the excrement impurities can be effectively avoided, and the excrement sample does not need to be subjected to precipitation treatment before the detection.