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Phosphate buffer solution and preparation method thereof

A phosphate buffer and buffer technology are applied in the field of phosphate buffer and its preparation, which can solve problems such as adverse effects of test results, and achieve the effects of reducing endotoxin load, simple configuration process and reducing fever reaction.

Active Publication Date: 2018-08-21
SHANGHAI HILEAP MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphate buffer solution configuration process on the market, the osmotic pressure of the produced buffer solution can reach 280-320smolL, the pH value can reach 7.1-7.2, generally used in glucose / lactic acid analyzer, molecular cloning and cell culture, pH=7.4, and human body The isotonic blood maintains a balanced isotonic state between the inner and outer fluids of the blood isotonic cells, and if one of them is abnormal, it will break the homeostasis of the internal environment and have an adverse effect on the test results

Method used

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  • Phosphate buffer solution and preparation method thereof
  • Phosphate buffer solution and preparation method thereof
  • Phosphate buffer solution and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0031] The preparation experimental apparatus of concentrate phosphate buffer solution is as follows:

[0032] (1) Stirrer;

[0033] (2) Digital thermostat;

[0034] (3) pH meter;

[0035] (4) Conductivity meter;

[0036] (5) 5L large volume cup;

[0037] (6) The test sample is placed in a 100ml small beaker;

[0038] (7) Insert the electrodes of the pH meter and conductivity meter into the solution after calibration;

[0039] (8) Press the "reading" button of the pH meter and the conductivity meter respectively;

[0040] (9) Wait for 1min-2min, record the reading on the pH meter and the reading on the conductivity;

[0041] (10) Dilute the concentrated solution again, dilute it 10 times, test the diluted solution on the machine, and compare and analyze it with some original technical indicators.

[0042] Citation: YYT 0456.1-2014 Reagents for blood analyzers Part 1: Cleaning solution; YYT 0456.3-2014 Reagents for blood analyzers Part 3: Diluents; ISO18113-2 Part 2: In ...

Embodiment 2

[0048]1. Put 3000ml of ultra-pure water in a 5000ml measuring cup (the volume needs to be calibrated, it has been calibrated), put the measuring cup under the stirrer, let the stirring rod extend to 1 / 2 of the liquid surface, set the stirring frequency to 55HZ, and start stirring , then add 0.56% potassium dihydrogen phosphate, 2.38% disodium hydrogen phosphate dihydrate, 1.40% potassium chloride, 0.197% sodium nitrate, 0.39% EDTA-2Na (dihydrate) and 0.053 TritonX-100 to the 5000ml measuring cup. %, K40 0.12%, each time you add a chemical, you must wait for it to dissolve completely before adding the next chemical, and wait until the above chemicals are completely dissolved, and then fill up the 5L measuring cup to the mark (already Calibrated scale line), continue to stir, after the stirring is finished, the solution can be sterilized and filtered to obtain the required concentrated solution,

[0049] 2. Stand still for 1 hour to obtain the pre-filling solution. The obtained ...

Embodiment 3

[0053] 1. Put 3000ml of ultra-pure water in a 5000ml measuring cup (the volume needs to be calibrated, it has been calibrated), put the measuring cup under the stirrer, let the stirring rod extend to 1 / 2 of the liquid surface, set the stirring frequency to 60HZ, and start stirring , then add potassium dihydrogen phosphate 0.57%, disodium hydrogen phosphate dihydrate 2.39%, potassium chloride 1.55%, sodium nitrate 0.197%, EDTA-2Na (dihydrate) 0.36%, TritonX-100 0.055 to the 5000ml measuring cup successively %, K40 0.13%, every time you add a chemical, you must wait for it to completely dissolve and then add the next chemical. After the above chemicals are completely dissolved, then fill up the 5L measuring cup to the mark (already Calibrated scale line), continue to stir, after the stirring is finished, the solution can be sterilized and filtered to obtain the required concentrated solution,

[0054] 2. Stand still for 1 hour to obtain the pre-filling solution. The obtained con...

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Abstract

The invention discloses a preparation method for a phosphate buffer solution. 5 L of the concentrated phosphate buffer solution comprises potassium dihydrogen phosphate with an addition concentrationof 0.55 wt%, disodium hydrogen phosphate dihydrate with an addition concentration of 2.37-2.38 wt%, potassium chloride with an addition concentration of 1.50 wt%, sodium nitrate with an addition concentration of 0.19%-0.20 wt%, disodium edetate dihydrate with an addition concentration of 0.38-0.40 wt%, Triton X-100 with an addition concentration of 0.05-0.055 wt%, and water used as a solvent. During preparation of the phosphate buffer solution of the invention, a large amount of foam is not generated; and the method is simple to operate and greatly improved in efficiency, and can easily control the pH value and conductivity of the phosphate buffer solution.

Description

technical field [0001] The invention relates to a phosphate buffer solution and a preparation method thereof. Background technique [0002] The main component of the concentrated solution is phosphate buffer saline (phosphate buffer saline), which is generally used as a solvent to dissolve and protect reagents. It is the most widely used buffer solution in biochemical research, and its main components are Na2HPO4.2H2O, KH2PO4, NaNO3, KCl, TritonX-100, etc. It is used as a 10-fold concentrated solution for in vitro diagnostic reagents, so its technical indicators are mainly studied It involves PH value and conductivity (conductivity indirectly reflects the osmotic pressure of the solution, because the osmotic pressure depends on the number of molecules of the solute), and according to related technologies, it is found that the PH value is 6.8-7.3 and the conductivity is 42-44ms / cm The test results obtained by diluting the 10-fold concentrated solution of the range into the d...

Claims

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Application Information

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IPC IPC(8): G01N1/38
CPCG01N1/38
Inventor 刘峻
Owner SHANGHAI HILEAP MEDICAL TECH CO LTD
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