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Microspore induced culture medium and method of celery

The technology of an induction medium and a culture method, which is applied to the field of celery microspore induction medium and microspore induction culture, can solve the problems that the celery microspore cultivation work has not been carried out, the celery microspore culture system has not been established, etc. Induction rate of wounded tissue and effect of promoting growth and development

Inactive Publication Date: 2019-04-19
江苏润知农业技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few domestic literature reports on the culture of celery microspores, the culture system of celery microspores has not yet been established, and the work of celery meploidy breeding has not yet been carried out.

Method used

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  • Microspore induced culture medium and method of celery
  • Microspore induced culture medium and method of celery
  • Microspore induced culture medium and method of celery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Carry out celery microspore induction culture test according to technical scheme of the present invention, concrete steps are as follows:

[0026] (1) Flower bud selection and pretreatment: celery varieties Ventura and Jinqi No. 1 were used as test materials, and flower buds from healthy plants were collected from 7:00 to 9:00 am on a sunny day during the normal budding and flowering season of celery, and used Acetate magenta staining method is used to identify the development stage of microspores. The specific operation is as follows: pick 1-3 anthers in the flower buds and crush them, release pollen, stain with acetic acid magenta and observe under the microscope, and select microspores in the mononuclear marginal stage The flower buds were wrapped with moist gauze, and then placed in a 4°C refrigerator for low-temperature pretreatment for 3 days.

[0027] (2) Preparation of microspore suspension: Rinse the above flower buds with tap water for about 30 minutes, then p...

Embodiment 2

[0031] The effect of different induction media on the induction rate of microspore callus: using plant tissue culture conventional media NLN, MS, B5, W14, N6 and adding 2,4-D 0.3mg / L respectively as induction media, according to the implementation The method for inducing callus by microspores provided in Example 1 was tested, and the callus induction rates of two varieties, Ventura and Jinqi No. 1, were counted. The results are shown in Table 1 below.

[0032]

[0033] As can be seen from Table 1, using the induction medium of the present invention, the callus induction rate of Ventura reaches 9.39%, and the callus induction rate of Jinqi No. 1 reaches 17.44%, which are significantly higher than those using other media callus induction rate. The difference in induction rate between Ventura and Jinqi No. 1 varieties was mainly due to the difference in genotype. The induction medium of the present invention has adjusted the ratio of each nutrient element, adjusted the carbon...

Embodiment 3

[0035] The influence of different days of low temperature pretreatment on the induction rate of microspore callus: the test was carried out according to the method of microspore induction of callus provided in Example 1, wherein the number of days of low temperature pretreatment in step (1) was set to 1d, 2d, On 3d and 4d, no low-temperature pretreatment was used as the control, and other operations remained unchanged. The callus induction rates of the two varieties of Ventura and Jinqi 1 were counted, and the results are shown in Table 2 below.

[0036]

[0037] It can be seen from Table 2 that within a certain range, with the increase of the days of low temperature pretreatment, the callus induction rate of Ventura and Jinqi No. 1 varieties also gradually increased, and the induction rate reached the highest value on the 3rd day , and then the callus induction rate decreased gradually, indicating that proper low temperature pretreatment is beneficial to increase the callus...

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Abstract

The invention provides a microspore induced culture medium and method of celery. The microspore induced culture medium herein is composed of suitable macro elements, suitable trace elements, iron salts, organic components, a promoting factor and a plant growth regulator. The microspore induced culture method includes the steps of (1) selecting and pretreating buds; (2) preparing a microspore suspension; (3) performing microspore induced culture, with specific electromagnetic wave radiation carried out during the microspore induced culture. The microspore induced culture medium is dedicated tomicrospore culture of celery; microspore callus of celery can be successfully attained through the medium in combination of an induction method herein; material basis is laid for future haploid breeding of celery.

Description

technical field [0001] The invention belongs to the field of agricultural science and technology, and in particular relates to a celery microspore induction medium and a microspore induction culture method. Background technique [0002] Celery, also known as celery, early celery, parsley, cattail celery, medicinal celery, and wild coriander, is a herbaceous plant in the genus Celery of the Umbelliferae family. It is native to the Mediterranean coast and was introduced to my country in the Han Dynasty. As a traditional vegetable, celery is not only nutritious, crisp and delicious, but also can cure diseases and keep fit. It is known as a good vegetable and medicine. In the past 20 to 30 years, with the improvement of people's quality of life, the consumption of celery has continued to increase, and the planting area has also expanded accordingly. Now it has become one of the most important vegetables in my country. At present, celery on the market is roughly divided into two...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨鑫鲍樱文
Owner 江苏润知农业技术服务有限公司
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