Cell cryopreservation protection composition, use thereof and cell cryopreservation method

Abstract

The invention relates to a cell cryopreservation protection composition, use thereof and a cell cryopreservation method. The cell cryopreservation protection composition contains amphipathic molecules with a general formula structure of R1-N+ (CH3)2-(CH2) n-R2, wherein R1 is alkyl of a straight chain or a branch chain of the carbon numbers 1 to 10, and is optionally substituted by a substituent; R2 is selected from an optional group with negative charge in a group consisting of COO-, -SO4-, -SO3- and a compound as shown in the description; R3 is selected from a group in a group consisting of (methyl) acryoyloxyalkyl, alkyl and alkenyl; and the amphipathic molecules with the general formula structure of R1-N+ (CH3)2-(CH2) n-R2 are a betaine compound preferably. The cell cryopreservation protection composition can implement cell cryopreservation non-toxically and efficiently, and after resuscitation of cells, the yield of the cells is quite high, and gradient cryopreservation is not required; and after resuscitation of the cells, the cells can be directly used or are slightly diluted and then used.

Description

technical field [0001] The application belongs to the field of cell biology, and specifically relates to the use of a 1 -N + (CH 3 ) 2 -(CH 2 ) n -R 2 Amphiphile, non-toxic and efficient cell cryoprotective composition. The present application also relates to the use of the composition and a method for cryopreserving cells. Background technique [0002] With the development of biomedical technology, cells isolated from living tissue or blood are more and more widely used in various fields of biomedicine. Living cells have not only been widely used in basic research in universities, research institutes and other research institutions. In the field of pharmaceutical development, living cells are used to investigate the efficacy, dosage and toxicity of drugs, provide direction guidance for animal experiments, and also reduce the amount of experimental animals and reduce risks. In the medical field, on the one hand, living cell samples obtained from the human body can b...

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Problems solved by technology

[0008] The purpose of the present invention is to solve the shortcomings of the existing cell cryopreservation technology, such as high toxicity of cryopreservation agents, cumbersome operation, unstable cryopreservation (hereinafter referred to as "cryopreservation") efficiency, etc. 1 -N + (CH 3 ) 2 -(CH 2 ) n -R 2 Amphiphilic molecules, a cell cryopreservation composition without the assistance of any cytotoxic cell cryopreservation components (such as glycerol or DMSO), and the use of the composition and the cryopreservation of cells using the composition method

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