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Cell cryopreservation protection composition, use of the composition and method for cell cryopreservation

A composition and cell technology, applied in the field of cell biology, can solve the problems of complicated operation, high toxicity of cryoprotectant, unstable cryopreservation efficiency and the like

Active Publication Date: 2018-06-08
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to solve the shortcomings of the existing cell cryopreservation technology, such as high toxicity of cryopreservation agents, cumbersome operation, unstable cryopreservation (hereinafter referred to as "cryopreservation") efficiency, etc. 1 -N + (CH 3 ) 2 -(CH 2 ) n -R 2 Amphiphilic molecules, a cell cryopreservation composition without the assistance of any cytotoxic cell cryopreservation components (such as glycerol or DMSO), and the use of the composition and the cryopreservation of cells using the composition method

Method used

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  • Cell cryopreservation protection composition, use of the composition and method for cell cryopreservation
  • Cell cryopreservation protection composition, use of the composition and method for cell cryopreservation
  • Cell cryopreservation protection composition, use of the composition and method for cell cryopreservation

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preparation example

[0102] Unless otherwise specified, the reagents, raw materials, and cells used in the preparation examples and effect examples of this application can be purchased from the market, or prepared by conventional methods in the art.

[0103] source of material

[0104] Betaine (from Inokai Acros, product number: 204241000)—compound 1;

[0105] Sulphobetaine (from Siensi, product number: M-58510) - compound 4;

[0106] Phosphorylcholine derivatives (from Siensi, product number: P-013898)—compound 5;

[0107] L-carnitine (sourced from Dalian Meilun, product number: MB3278)—Compound 6.

[0108] (3-Acrylamidopropyl)-(2-carboxyethyl)-dimethylammonium salt (compound 2), its preparation method is as follows: use a 500mL three-necked flask to purge the system with nitrogen for 15min to remove air and Water vapor; add 300mL of anhydrous acetone, add 53.98g of dimethylaminoethyl methacrylate, stir at room temperature for half an hour until completely dissolved, protect with nitrogen, t...

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Abstract

The present application relates to a cell cryopreservation protection composition, the use of the composition and a cell cryopreservation method. The cryopreservation composition for cells comprises an amphiphilic molecule having a general structure R1-N+(CH3)2-(CH2)n-R2, wherein the above-mentioned R1 is a linear or branched alkyl group with 1 to 10 carbons, It is optionally substituted by a substituent, R2 is any negatively charged group selected from the group consisting of -COO-, -SO4-, -SO3-, and the group consisting of (methyl) A group in the group consisting of acryloyloxyalkyl, alkyl, and alkenyl; the amphiphilic molecule with the general structure R1-N+(CH3)2-(CH2)n-R2 is preferably a betaine compound . The cell cryopreservation protection composition can carry out cell cryopreservation in a non-toxic and efficient manner, and the survival rate of cells after thawing is extremely high without gradient cryopreservation; after thawing cells, the cells can be used directly or slightly diluted.

Description

technical field [0001] The application belongs to the field of cell biology, and specifically relates to the use of a 1 -N + (CH 3 ) 2 -(CH 2 ) n -R 2 Amphiphile, non-toxic and efficient cell cryoprotective composition. The present application also relates to the use of the composition and a method for cryopreserving cells. Background technique [0002] With the development of biomedical technology, cells isolated from living tissue or blood are more and more widely used in various fields of biomedicine. Living cells have not only been widely used in basic research in universities, research institutes and other research institutions. In the field of pharmaceutical development, living cells are used to investigate the efficacy, dosage and toxicity of drugs, provide direction guidance for animal experiments, and also reduce the amount of experimental animals and reduce risks. In the medical field, on the one hand, living cell samples obtained from the human body can b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226C07C229/22C07C237/50C12N5/0695
Inventor 张雷杨静
Owner TIANJIN UNIV
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