Preparation method for microvesicle composite regenerative medical material
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of microbubbles and scaffold materials, applied in medical science, prostheses, etc., can solve the problems of seed cell growth and premature apoptosis, and achieve the effect of promoting angiogenesis, simple cryopreservation method, and less immune rejection
Inactive Publication Date: 2015-10-14
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
View PDF1 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] The purpose of the present invention is to provide a preparation method of microbubble composite regenerative medical materials to solve the problem of premature apoptosis of seed cells due to the failure of blood vessels to grow in time during the tissue engineering construction process. provide new ideas
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0025] Example 1 Preparation of microbubble / decalcified bone matrix composite regenerative medical material
[0026] 1. Extraction of rat bone marrow MSC-derived MV
[0027] SD rat bone marrow-derived MSCs were cultured in primary culture, and MSCs from the 3rd to 5th passages were used to extract MVs. Before extracting MVs, the MSC medium was replaced with a serum-free medium to continue culturing for 24 h. Collect the cell supernatant, centrifuge at 2,000×g for 20 minutes, discard the pellet and take the pellet, centrifuge at 100,000×g, 4°C for 1 hour, discard the supernatant, resuspend the pellet in PBS, and freeze at minus 80°C for later use.
[0028] 2. Preparation of MV / DBM composite regenerative medical materials
[0029] 1) The biological material used in this example is the decalcified bone matrix (DBM) commonly used in tissue engineering bone. The scaffold material was cut into a size of 3×3×3 mm, soaked in 75% alcohol for 2 hours, Wash 3 times with PBS and dry at...
Embodiment 2
[0033] Example 2 Preparation of the composite regenerative medical material of microbubble / polycaprolactone
[0034] 1. Extract mouse bone marrow MSC-derived MV
[0035]BALB / c mouse bone marrow-derived MSCs were cultured in primary culture, and MSCs from the 3rd to 5th passages were used to extract MVs. Before extracting MVs, the MSC medium was replaced with a serum-free medium to continue culturing for 24 h. Collect the cell supernatant, centrifuge at 2,000×g for 20 minutes, discard the pellet and take the pellet, centrifuge at 100,000×g, 4°C for 1 hour, discard the supernatant, resuspend the pellet in PBS, and freeze at minus 80°C for later use.
[0036] 2. Preparation of MV / PCL composite regenerative medical materials
[0037] 1) The biological material used in this example is polycaprolactone (polycaprolactone, PCL), which is commonly used in tissue engineering bone. The scaffold material was cut into a size of 3×3×3 mm, soaked in 75% alcohol for 2 hours, and washed with...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention belongs to the technical field of regenerative medicine and tissue engineering and discloses a preparation method for a microvesicle composite regenerative medical material. The preparation method comprises the following steps: (1), extracting mesenchymal stem cell derived microvesicles; and (2), inoculating microvesicles extracted in the step (1) to a tissue-engineered bone scaffold material, so as to obtain the microvesicle composite regenerative medical material through incubation. According to the invention, the mesenchymal stem cell derived microvesicles are inoculated to the tissue-engineered bone scaffold material to obtain a novel composite material, experiment results show that the composite material can increase the survival rate of seed cells in vivo, and the difficulty of early apoptosis of seed cells because blood vessels cannot grow in timely in a tissue engineering establishment process is solved. In addition, the mesenchymal stem cell derived microvesicles do not cause immunological rejection in vivo easily, have good biological safety, and can cross species to take a biological effect, therefore, the problem that a donor source is limited in the regenerative medicine engineering is solved.
Description
technical field [0001] The invention relates to the technical fields of regenerative medicine and tissue engineering, in particular to a preparation method of a microbubble composite regenerative medical material. Background technique [0002] The repair and functional reconstruction of tissue and organ defects are still difficult problems faced by modern medicine. Autologous or allogeneic tissue and organ transplantation are currently the most commonly used treatment methods, but there are shortcomings such as a serious shortage of donor sources. The concept of tissue engineering is expected to solve this problem. Tissue engineering is dedicated to the research on the technology of constructing human organs or tissues in vitro, using biodegradable and compatible materials to prepare 3D scaffolds that meet the shape of the required organs or tissues, so that the stem cells adsorbed on the materials gradually grow in a specific way. It grows into the required organs and tis...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/54
Inventor 谢慧陈智超张文杰汪振星
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
Login to view more 
Login to view more